Nonomuraea insulae sp. nov., isolated from forest soil

Abstract

Strain H2R21, a novel actinobacterium, isolated from a forest soil sample collected from Heybeliada, Istanbul, Turkey, and a polyphasic approach was used for characterisation of the strain. Chemotaxonomic and morphological characterisation of strain H2R21 indicated that it belongs to the genus Nonomuraea. 16S rRNA gene sequence similarity showed that the strain is closely related to Nonomuraea purpurea 1SM4-01 (99.1%) and Nonomuraea solani CGMCC 4.7037 (98.4%). DNA-DNA relatedness values were found to be lower than 70% between the isolate and its phylogenetic neighbours N. purpurea 1SM4-01, N. solani CGMCC 4.7037 and Nonomuraea rhizophila YIM 67092. The whole cell hydrolysates of strain H2R21 were found to contain meso-diaminopimelic acid as the diagnostic diamino acid and glucose, madurose, mannose and ribose as the cell sugars. The polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylinositol, glycophosphatidylinositol, two glycophospholipids and two unidentified lipids. The predominant menaquinones were identified as MK-9(H) and MK-9(H). The major fatty acids were found to be iso-C, iso-C 2OH and C 10-methyl. On the basis of DNA-DNA relatedness data and some phenotypic characteristics, it is evident that strain H2R21 can be distinguished from the closely related species in the genus Nonomuraea. Thus, it is concluded that strain H2R21 represents a novel species of the genus Nonomuraea, for which the name Nonomuraea insulae sp. nov. is proposed. The type strain is H2R21 (= DSM 102915 = CGMCC 4.7338 = KCTC 39769).

Streptomyces ureilyticus sp. nov. and Streptomyces mesophilus sp. nov., two novel Actinobacteria with antimicrobial activity isolated from lake sediment

“…The phylogenomic tree () reconstructed on the TYGS (https://tygs.dsmz.de/) also revealed the tendency of the strains to the genus Streptomyces . DNA–DNA hybridization (DDH) analyses were carried out as detailed in the previous study [24] between the isolate YC419 T and its closely phylogenetic neighbour, S. vastus DSM 40309 T , the only strain that shares a 16S rRNA similarity value above the critical 98.65 % value [25].…”

“…de/) also revealed the tendency of the strains to the genus Streptomyces. DNA-DNA hybridization (DDH) analyses were carried out as detailed in the previous study [24] between the isolate YC419 T and its closely phylogenetic neighbour, S. vastus DSM 40309 T , the only strain that shares a 16S rRNA similarity value above the critical 98.65 % value [25]. The strains showed 54.3±2.7 % DDH relatedness, which is lower than 70 % cut-off for species delineation [26][27][28].…”

Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov.

“…In addition, it is known that Nonomuraea rosea, Nonomuraea roseoviolacea, Nonomuraea rubra, Nonomuraea pusilla, Nonomuraea spiralis and Nonomuraea gerenzanensis produce deoxycephalomycin B, carminomicins, maduromycin, actinotiocin, pyralomicin and glycopeptide antibiotic A40926 [9][10][11][12][13][14][15]. Members of the genus have been isolated from diverse natural habitats such as rhizosphere [16,17], leaf [18], cave [19], limestone mine [20], root [5,21], coastal sediment [22], mangrove sediment [23] and mushroom compost [24], while many members were isolated from different types of soil including cave soil [25], forest soil [26,27], arid soil [28], acidic soil [29], rhizosphere soil [3,[30][31][32] and mangrove soil [33].…”