A Gram-stain-positive, aerobic, non-motile, rod-shaped, yellow-pigment-producing bacterium, (designated strain CC-CZW004 T ), was isolated from seafood samples (sea urchins) at Penghu Island in Taiwan. Strain CC-CZW004 T grew optimally at pH 7.0 and 30 8C in the presence of 3 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CZW004 T with respect to other members of the genus Nocardioides. The novel strain shared highest 16S rRNA gene sequence similarities to Nocardioides daejeonensis JCM 16922 T (96.4 %), Nocardioides pacificus JCM 19260 T (96.3 %), and Marmoricola scoriae JCM 17444 T (96.2 %). The major fatty acids of strain CC-CZW004 T consisted of C 17:0 , C 16:1 v5c, C 17:1 v8c', iso-C 16:0 and C 19:1 v11c/C 19:1 v9c (summed feature 6). The diagnostic diamino acid in the cell wall was LL-2,6-diaminopimelic acid. The polar lipid profile was composed of major amounts of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 73.3 mol%. The predominant quinone system was menaquinone . On the basis of polyphasic taxonomic evidences presented here, strain CC-CZW004 T represents a novel species of the genus Nocardioides, for which the name Nocardioides echinoideorum sp. nov. is proposed. The type strain is CC-CZW004 T (5BCRC 16974 T 5JCM 30276 T ).The genus Nocardioides, which is the type genus of the family Nocardioidaceae, was first proposed by Prauser (1976) with type species Nocardioides albus. Species of the genus Nocardioides are represented by aerobic, Gram-stain-positive rods, with high DNA G+C content (68.7-74.9 mol%). The major respiratory quinone is menaquinone MK-8(H 4 ) and LL-2,6-diaminopimelic acid is the diagnostic diamino acid of the peptidoglycan. The fatty acid profile contains both branched-and straight-chain fatty acids (O'Donnell et al., 1982), and iso-C 16:0 as the dominant fatty acid. At the time of writing, there are 73 species of the genus Nocardioides with validly published names, according to the List of Prokaryotic names with Standing in Nomenclature (LPSN; http://www.bacterio.net/nocardioides.html) website. These species are widely distributed in various terrestrial and aquatic environments, including marine sediment, and may be associated with plants, animals and humans (Yi & Chun, 2004a, b;Dastager et al., 2009;Evtushenko et al., 2012). Recently described species of the genus Nocardioides include Nocardioides zeae (Glaeser et al., 2014), Nocardioides pacificus (Fan et al., 2014), Nocardioides soli (Sun et al., 2014), Nocardioides nanhaiensis and Nocardioides opuntiae (Lee & Seong, 2014), which were isolated from the stem of Zea mays, deep sub-seafloor sediment, soil, marine sediment and soil of cactus, respectively.In this study, another Nocardioides strain was isolated from seafood samples (sea urchins) collected at Penghu Island (238 359 N 1198 359 E) in Taiwan, and was characterized taxonomically. Sea urchin shells were introduced into sterile wa...