Multilocus genetic structure, characterization, and relationships of <i>Populus</i> × <i>canadensis</i> cultivars

Rajora, Om P.; Zsuffa, L.

doi:10.1139/g89-415

Cited by 32 publications

(41 citation statements)

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“…A great amount of useful information for genetic characterization and identification of poplars has accumulated, coming from the use of both allozyme polymorphism [28,30] or DNA markers: RAPDs [4,23,29,31] AFLPs, [1,6,8,15,40], microsatellites (SSRs) [3,6,10,14,30] and ISSRs [20].…”

Section: Introductionmentioning

confidence: 99%

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

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-In order to protect the legitimate interests of the poplar breeders, growers and industries, we have developed a reliable and affordable certification tool for commercial poplars in Spain. We have carried out a genetic description of the 28 commercial clones of the current Spanish National Catalogue using 10 microsatellite molecular markers (SSR). The results clearly show that in the Spanish National Catalogue there are labelling mistakes including synonymies, supported by a combined probability of identity (PI) of 1.18 × 10 −9 . We have also demonstrate that it is possible to identify all the different clones (genotypes) with only three microsatellites, that show a combined probability of identity of 2.4 × 10 −4 , and we have optimised the protocols to reduce the time and the economical costs of the analysis. This SSR-based tool could be incorporated in the current certification protocols, to avoid duplication of accessions and identification mistakes.commercial poplar clone / microsatellite / identification / Spanish national catalogue Résumé -Développement de marqueurs microsatellites pour l'identification et la certification des clones commerciaux de peuplier en Espagne. Dans le but de protéger les intérêts légitimes des cultivateurs, des sélectionneurs et des industriels de la populiculture, nous avons mis au point un outil de certification fiable et accessible pour les clones de peupliers commerciaux en Espagne. Nous avons fait une description génétique des 28 clones commerciaux de l'actuel catalogue national espagnol en utilisant 10 marqueurs moléculaires microsatellites (SSR). Les résultats obtenus indiquent clairement que ce catalogue contient des erreurs d'identification, dont des clones synonymes, soutenues par une probabilité combinée d'identité (PI) de 1.18 × 10 −9 . Nous avons également démontré qu'il est possible d'identifier tous les génotypes différents avec 3 microsatellites, qui montrent une probabilité combinée d'identité de 2.4 × 10 −4 , et nous avons optimisé les protocoles afin de réduire le temps et le coût de l'analyse. Cet outil fondé sur les SSR pourrait être intégré dans les protocoles actuels de certification afin d'éviter la duplication des matériaux et les erreurs d'identification.clone commercial de peuplier / microsatellite / identification / catalogue national espagnol

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Section: Introductionmentioning

confidence: 99%

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

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“…deltoides, P. maximowiczii and P . X canadensis (Rajora, 1986 ;Rajora & Zsuffa, 1989), and in Cryptomeria japonica (Miyazaki & Miyajima, 1981) . Isozyme markers have been found to be appropriate for identification of cultivars, and commercial and botanical varieties of several agricultural and related plants (e .g ., Fedak, 1974 ;Bassiri & Rouhani, 1977 ;Bassiri & Adams, 1978 ;Cardy & Kannenberg, 1982 ;Ignart & Weeden, 1984 ;Weeden, 1984 ;Nielsen, 1985 ;Nielsen & Johansen, 1986 ; among others) .…”

Section: Discussionmentioning

confidence: 99%

“…We have demonstrated allozymes as potential tools for clonal and cultivar identification of Populus deltoides, P. maximowiczii Henry and P . X canadensis (Rajora, 1986 ;Rajora & Zsuffa, 1989 (Rajora, 1986), modified from Shaw & Prasad (1970) ; and Siciliano & Shaw (1976), and incubating at 37C . Gel electrophoresis experiments were repeated four times in independent sampling in two years and repeatable results were obtained .…”

Section: Introductionmentioning

confidence: 99%

Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

Rajora

1989

Euphytica

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Methods of horizontal starch gel electrophoresis of enzymes in root tips of Populus nigra were developed .The genetic structure of 43 P. nigra clones representing selections, cultivars and botanical varieties from 9European countries was determined for 36 allozyme loci coding for 11 enzyme systems in root tips . All the clones were identical at 26 loci and the interclonal allozyme variability was controlled by 10 loci . Forty-two unique multilocus genotypes were observed among the 43 clones . On average, unique genotypes differed from each other at 3 .42 loci . Principal Component Analysis of the clonal allozyme genotypes for 10 polymorphic loci indicated 5 loci (6-Pgd-4, 6-Pgd-5, Idh-2, Per-2 and Mdh-4) to be the most differentiating among the clones . The relationships among the clones as portrayed by their ordination on Principal Components 1 and 2 were presented . Several clones from the same country, especially from the Netherlands, tended to group together . The results have utility in identification, certification and registration of P . nigra clones and in planning an effective breeding program .

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“…Genetic similarity coefficients among the clones were determined by calculating Jaccard's similarities (Jaccard 1908) based on the raw data matrix as follows: GS = 2N ij /(N i + N j ), where GS is the genetic similarity coefficient, N ij is the number of alleles in common between clones i and j, and N i and N j are the total number of alleles observed for clones i and j, respectively. PCA was performed by using SPSS 17.0 (Coakes and Steed 2009;Gao et al 2009) following the description in Rajora's (1989) paper, and principal component 1 and 2 was extracted for visualizing the ordination of these clones.…”

Section: Analyzing the Genetic Relationshipmentioning

confidence: 99%

“…However, traditional observations are difficult, time consuming, and often subject to errors owing to environmental factors affecting the expression of these characteristics. In addition to these morphological markers, biochemical markers such as isozymes, which are based on multilocus allozyme genotypes, can be used to identify clones of P. deltoides and some other poplar species (Rajora 1988(Rajora , 1989aRajora and Zsuffa 1989;Rajora and Dancik 1992). Nonetheless, this type of marker is of low efficiency and reliability (Dayanandan et al 1998) because isozymes are not genetic materials but products of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Genetic identification of 43 elite clonal accessions ofPopulus deltoidesby SSR fingerprinting

et al. 2016

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In this study, 43 elite clones of Populus deltoides were fingerprinted with ABI 3730 capillary electrophoresis by using six SSR primer pairs. Based on the fingerprinting profiles, 62 polymorphic bands were generated with a mean number of 10 alleles per locus, and allele numbers amplified by each primer in these clones ranged from 5 (NJFUP-poly10) to 16 (NJFUP-poly07). The power of discrimination values for these primer pairs ranged from 0.80 to 0.94, with an average value of 0.89. Among the six primer pairs, the most efficient primer pair for genetic discrimination of these elite clones was NJFUP-poly02, which could identify 22 of the 43 elite clones directly. In conclusion, all the 43 elite clones of P. deltoides could be discriminated unambiguously based on their genotypes at the six SSR loci. The combination of all six loci gave considerable reliability and accuracy for genetic identification of these clonal accessions. Subsequently, genetic relationship of these clones was plotted by the UPGMA clustering and principle component analysis. Results of both analyses indicated that clone "C7-1" had the largest genetic distance from the other clones, followed by clone "C51-1" and a subgroup comprising clone "C100-3" and "C62-3". These clones are proposed to be possibly more affected by the inter-specific gene introgression.Key words: Populus deltoides, clone identification, SSR fingerprinting, UPGMA cluster, PCA plot.Résumé : Dans cette étude, 43 clones élite de Populus deltoides ont été marqués avec six paires d'amorces SSR par électrophorèse capillaire au moyen d'un appareil ABI 3730. D'après la cartographie peptidique, la technique aboutit à 62 bandes polymorphes comprenant en moyenne 10 allèles par locus, le nombre d'allèles amplifiés dans chaque amorce variant de 5 (NJFUP-poly10) à 16 (NJFUP-poly07) chez les clones. La valeur discriminatoire des paires d'amorces variait de 0,80 à 0,94, pour une moyenne de 0,89. Sur les six paires d'amorces, celle permettant la meilleure discrimination génétique des clones élite était NJFUP-poly02, puisqu'elle a permis d'identifier directement 22 clones sur les 43. En conclusion, on est parvenu à distinguer sans ambiguïté les 43 clones élite de P. deltoides d'après leur génotype aux six locus SSR. Combiner les six locus rend l'identification génétique extrêmement fiable et précise pour ces obtentions, issues du clonage. Par la suite, les liens génétiques entre les clones ont été tracés après regroupement par la méthode des moyennes par paire non pondérée (UPGMA) et l'analyse en composantes principales. Dans les deux cas, les résultats indiquent que le clone C7-1 est génétiquement le plus éloigné des autres. Suivaient le clone C51-1 et un sous-groupe composé des clones C100-3 et C62-3. Il se peut que ces clones soient plus affectés par l'introgression des gènes interspécifiques. [Traduit par la Rédaction]

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Multilocus genetic structure, characterization, and relationships of Populus × canadensis cultivars

Cited by 32 publications

References 19 publications

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

Genetic identification of 43 elite clonal accessions ofPopulus deltoidesby SSR fingerprinting

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