Genetic identification of 43 elite clonal accessions ofPopulus deltoidesby SSR fingerprinting

Abstract

In this study, 43 elite clones of Populus deltoides were fingerprinted with ABI 3730 capillary electrophoresis by using six SSR primer pairs. Based on the fingerprinting profiles, 62 polymorphic bands were generated with a mean number of 10 alleles per locus, and allele numbers amplified by each primer in these clones ranged from 5 (NJFUP-poly10) to 16 (NJFUP-poly07). The power of discrimination values for these primer pairs ranged from 0.80 to 0.94, with an average value of 0.89. Among the six primer pairs, the most efficient primer pair for genetic discrimination of these elite clones was NJFUP-poly02, which could identify 22 of the 43 elite clones directly. In conclusion, all the 43 elite clones of P. deltoides could be discriminated unambiguously based on their genotypes at the six SSR loci. The combination of all six loci gave considerable reliability and accuracy for genetic identification of these clonal accessions. Subsequently, genetic relationship of these clones was plotted by the UPGMA clustering and principle component analysis. Results of both analyses indicated that clone "C7-1" had the largest genetic distance from the other clones, followed by clone "C51-1" and a subgroup comprising clone "C100-3" and "C62-3". These clones are proposed to be possibly more affected by the inter-specific gene introgression.Key words: Populus deltoides, clone identification, SSR fingerprinting, UPGMA cluster, PCA plot.Résumé : Dans cette étude, 43 clones élite de Populus deltoides ont été marqués avec six paires d'amorces SSR par électrophorèse capillaire au moyen d'un appareil ABI 3730. D'après la cartographie peptidique, la technique aboutit à 62 bandes polymorphes comprenant en moyenne 10 allèles par locus, le nombre d'allèles amplifiés dans chaque amorce variant de 5 (NJFUP-poly10) à 16 (NJFUP-poly07) chez les clones. La valeur discriminatoire des paires d'amorces variait de 0,80 à 0,94, pour une moyenne de 0,89. Sur les six paires d'amorces, celle permettant la meilleure discrimination génétique des clones élite était NJFUP-poly02, puisqu'elle a permis d'identifier directement 22 clones sur les 43. En conclusion, on est parvenu à distinguer sans ambiguïté les 43 clones élite de P. deltoides d'après leur génotype aux six locus SSR. Combiner les six locus rend l'identification génétique extrêmement fiable et précise pour ces obtentions, issues du clonage. Par la suite, les liens génétiques entre les clones ont été tracés après regroupement par la méthode des moyennes par paire non pondérée (UPGMA) et l'analyse en composantes principales. Dans les deux cas, les résultats indiquent que le clone C7-1 est génétiquement le plus éloigné des autres. Suivaient le clone C51-1 et un sous-groupe composé des clones C100-3 et C62-3. Il se peut que ces clones soient plus affectés par l'introgression des gènes interspécifiques. [Traduit par la Rédaction]

Molecular identification of mung bean accessions (Vigna radiata L.) from Northeast China using capillary electrophoresis with fluorescence‐labeled SSR markers

“…As one of the most popular molecular technologies, SSR markers have the advantages of high polymorphism, codominance, and good reproducibility compared with other technologies and thus play a significant role in genetic assessment (Cheng et al, 2011). Therefore, SSR have been widely applied for identification and diversity analysis in many crop species, including rice (Ahmed, Joel, Wariara, & Steven, 2018), soybean (Chakraborty, Patel, Parmar, Dhaduk, & Sasidharan, 2018), cotton (Bilwal, Vadodariya, & Rajkumar, 2017), and Ethiopian cowpea (Gupta & Gopalakrishna, 2010), and many other plant species, including cherry (Liang et al, 2018), sweet potato , and Populus deltoides (Liu et al, 2016). For mung bean, there have been some research reports on genetic diversity (Chontira, Akito, Norihiko, Duncan, & Peerasak, 2007), genetic linkage map construction (Liu, Liu, et al, 2017) and disease susceptibility (Akbar, Aslam, Atif, & Nawaz-Ul-Rehman, 2017), but SSR fingerprinting for identification has been rarely reported.…”

Identification and expression patterns of chemosensory proteins in the black-back prominent moth, Clostera restitura (Lepidoptera: Notodontidae)

“…Clostera restitura Walker (Lepidoptera: Notodontidae) is one of the most destructive defoliators of poplar trees in China. This pest is widespread in southern China, especially in forest-rich provinces, such as Anhui, Jiangsu, Zhejiang, Shanghai, Fujian, Guangxi, Guang dong, Hunan and Hainan (Zhang, 1997;Liu et al, 2016;Fang et al, 2018;Xin et al, 2018). It also occurs widely in other Asian countries including India, Indonesia, Malaysia and Vietnam (Wu & Fang, 2003;Schintlmeister, 2008).…”