Identification and expression patterns of chemosensory proteins in the black-back prominent moth, Clostera restitura (Lepidoptera: Notodontidae)

Abstract

http://www.eje.cz (OBPs), chemosensory proteins (CSPs), odour receptors (ORs), odour degrading enzymes (ODEs), ion receptors (IRs) and sensory neuron membrane proteins (SNMPs), participate in chemical perception (Leal, 2013; Cao et al., 2014; El fekih et al., 2016, Fleischer et al., 2018). Volatile chemical signals and other stimuli of lipophilic compounds cannot be directly transported to chemosensory receptors across hydrophilic lymph and must be bound by OBPs and CSPs (Yi et al., 2014a). Therefore, OBPs and CSPs, which are known as carrier proteins, play key roles in insect olfaction (Dani et al., 2011). CSPs and well-studied OBPs, are low-molecular-mass and soluble proteins used in insect chemoreception (Pelosi et al., 2006). CSPs are characterized by four cysteine residues (C 1-X 6-8-C 2-X 16-21-C 3-X 2-C 4) that form two disulfi de bridges (Tomaselli et al., 2006). Similar to OBPs, the rigid hydrophobic pocket in CSPs can capture and transport external chemical cues to receptors. CSPs were fi rst discovered in the antennae of Drosophila melanogaster (Mckenna et al., 1994). Further studies revealed that CSPs were expressed not only in antennae but also in other insect Identifi cation and expression patterns of chemosensory proteins in the black-back prominent moth, Clostera restitura

Research Progress on Oviposition-Related Genes in Insects

“…At present, the major genes related to olfactory proteins and receptors in female insects are Odorant-binding proteins ( OBPs ), Chemosensory proteins ( CSPs ), Odorant-degrading enzymes ( ODEs ), Odorant receptors ( ORS ), Ionotropic receptors ( IRs ), and Sensory neuron membrane proteins ( SNMPs ). The proteins encoded by these genes are the main proteins responsible for chemical signaling ( Leal 2013 , Cheng et al 2019a , González et al 2019 , Li et al 2019 , Zhang et al 2019 ). Among them, the research on OBPs is the most detailed, including the construction of a phylogenetic tree of OBP genes among different species of female insects; transcriptome analysis and the functions of related proteins have also been reported.…”

Transcriptome analysis of megalurothrips usitatus (Bagnall) identifies olfactory genes with ligands binding characteristics of MusiOBP1 and MusiCSP1

“…MusiCSP1 had the highest relative expression level in the pre-pupal stage, which is the transitional stage from the active nymph to the pupal stage. A previous study showed that CresCSP8 is highly expressed in the forth and fifth nymphs of Clostera restitura ( Li H. et al, 2019b ), and that CSP1 is the most highly expressed gene in the egg and pupal stages of Anomala corpulenta ( Chen et al, 2019 ). It has been hypothesized that chemosensory protein genes are related to growth and development, which would align with the expression pattern of MusiCSP1.…”

Sensory Geneidentificationin the Transcriptome of the Ectoparasitoid Quadrastichus Mendeliinthe Eucalyptus Gall wasp Leptocybe Invasa

“…In this study, 10 candidate GRs were identi ed, which was similar to other parasitoids, such as An. japonicus (8) 13 , Sclerodermus sp. (6) 2 and M. mediator (6) 8 .…”

“…chilonis Matsumura 6 , Microplitis demolitor Wilkinson 7 , M. mediator Haliday 8 , Microcentrus cingulum Brischke 9 , Aphidius gifuensis Ashmead 10 , Ap. ervi Haliday 11 and Meteorus pulchricornis Wesmael 12 ; Encyrtidae, e.g., Anastatus japonicus Ashmead 13 and Aenasius bambawalei Hayat 14 ; Eupelmidae, e.g., Copidosoma oridanum Ashmead 15 ; Ichneumonidae, e.g., Campoletis chlorideae Uchida 1 ; Trichogrammatidae, e.g., Trichogramma dendrolimi Matsumura 16 and Tric. japonicun Ashmead 3 ; Eulophidae, e.g., Asecodes hispinarum Boucek and Chouioia cunea Yang 4,17 .…”