Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

Rajora, Om P.

doi:10.1007/bf00023054

Cited by 29 publications

(25 citation statements)

References 23 publications

(33 reference statements)

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“…2), were used to absorb enzyme extracts. Populus xcanadensis samples were initially assayed on the gels in a block between those of P. deltoides and P. nigra samples and later with othei P. deltoides x P. nigra clones developed by controlled hybridization in North America, as reported in Rajora (1986). Red food color was used to indicate the migration front.…”

Section: Electrophoresis and Enzyme Detectionmentioning

confidence: 99%

“…Populus nigra samples consisted of 43 individual clones originated in nine European countries: The Netherlands, Hungary, Czechoslovakia, West Germany, Austria, France, England, Italy, and Yugoslavia (Rajora 1989b).…”

Section: Cultivars and Clonesmentioning

confidence: 99%

“…, Fedak 1974;Bassiri 1977;Bassiri and Rouhani 1977;Cardy and Kannenberg 1982;Torres 1983;Ignart and Weeden 1984;Weeden 1984;Nielsen and Johansen 1986;Ramirez et al 1987). Among forest trees, allozymes have been proven effective genetic markers for clonal identification in Populus deltoides (Rajora 1989a), P. nigra (Rajora 1989b), P. maximowiczii Henry (Rajora 1988), P. tremuloides Michx. (Cheliak and Pitel 1984), and Cryptomeria japonica (Miyazaki and Miyajima 198 1).…”

Section: Introductionmentioning

confidence: 99%

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Multilocus genetic structure, characterization, and relationships of Populus × canadensis cultivars

Rajora¹,

Zsuffa²

1989

Genome

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The genotypes of 21 clones of 18 Populus × canadensis Moench cultivars ('Baden 431', 'Blanc du Poitou', 'Canada Blanc', 'DN44', 'Dorskamp 925', 'Eugenei', 'Gelrica', 'Grandis', 'Heidemij', 'I-55/56', 'I-132/56', 'I-262', 'Jacometti', 'Ostia', 'Regenerata', 'Robusta', 'Steckby', and 'Zurich 03/3') were determined for 31 allozyme loci coding for 9 enzyme systems in root tips. Horizontal starch gel electrophoresis was used to assay the enzymes. All clones had allozyme gene and allele contribution from both P. deltoides Marsh, and P. nigra L. The interclonal allozyme variability was controlled by nine loci. 'Canada Blanc' and 'Ostia' shared the same 31-locus genotypes, whereas each of the remaining 19 clones, including 3 clones of 'Robusta' and 2 clones of 'Jacometti', had unique multilocus genotypes. On average, unique genotypes differed from each other at 3.59 loci. Principal-component analysis of the clonal genotypes at 9 variable loci indicated 4 loci (Idh-2, Idh-3, Mdh-4, and Idh-1) to be the most discriminating among the clones. The first two principal components accounted for 48% of the total variance in the nine loci. Clones on the principal component analysis ordination (principal components 1 and 2) were separated into 3 groups; 14 belonged to one group and 5 to a second group. 'Blanc du Poitou' was widely separated from all others. 'Canada Blanc' and 'Steckby' showed the highest genetic interrelationships. Three 'Robusta' clones and two 'Jacometti' clones were in the same group., The interrelationships among the clones based on allozyme genotypes were in general agreement with their speculated origin and (or) morphology.Key words: Populus × canadensis, allozymes, multilocus genotypes, clone–cultivar identification, clone relationships.

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Section: Electrophoresis and Enzyme Detectionmentioning

confidence: 99%

Section: Cultivars and Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multilocus genetic structure, characterization, and relationships of Populus × canadensis cultivars

Rajora¹,

Zsuffa²

1989

Genome

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“…Since the late 1980s, several molecular marker approaches have been successfully used in a number of poplar species for the fingerprinting and identification of clones and the determination of their interrelationship. Allozyme [10,12,27] and randomly amplified polymorphic DNA (RAPD) [5,17,31] analyses were initially used for this purpose because of their simplicity and relatively low cost. However, the small numbers of polymorphism present in allozyme and lack of reproducibility of RAPD limit the usefulness of these markers.…”

Section: Introductionmentioning

confidence: 99%

ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genusPopulus

Gao¹,

Zhang²,

Liwang³

et al. 2006

Ann. For. Sci.

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-Inter-simple sequence repeat polymorphism (ISSR) and amplified fragment length polymorphism (AFLP) analysis techniques were used in this study for the genetic fingerprinting and identification of 28 important Chinese poplar accessions. After fingerprinting, the genetic relationships among the accessions were determined. Each of three ISSR primers and four AFLP primer pairs produced fingerprint profiles that were unique to each of the accessions studied, and thus could be used solely for their identification. In general, the molecular data separated accessions from different poplar sections, and also distinguished between native and exotic accessions. In conclusion, both ISSR and AFLP could be applied to identify large numbers of poplar accessions, and could also be used to rapidly determine the genetic relationships among them. Furthermore, it is useful to conduct comparative studies with different marker systems when investigating the genetic relationships of poplar accessions.

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“…However, traditional observations are difficult, time consuming, and often subject to errors owing to environmental factors affecting the expression of these characteristics. In addition to these morphological markers, biochemical markers such as isozymes, which are based on multilocus allozyme genotypes, can be used to identify clones of P. deltoides and some other poplar species (Rajora 1988(Rajora , 1989aRajora and Zsuffa 1989;Rajora and Dancik 1992). Nonetheless, this type of marker is of low efficiency and reliability (Dayanandan et al 1998) because isozymes are not genetic materials but products of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Genetic identification of 43 elite clonal accessions ofPopulus deltoidesby SSR fingerprinting

et al. 2016

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In this study, 43 elite clones of Populus deltoides were fingerprinted with ABI 3730 capillary electrophoresis by using six SSR primer pairs. Based on the fingerprinting profiles, 62 polymorphic bands were generated with a mean number of 10 alleles per locus, and allele numbers amplified by each primer in these clones ranged from 5 (NJFUP-poly10) to 16 (NJFUP-poly07). The power of discrimination values for these primer pairs ranged from 0.80 to 0.94, with an average value of 0.89. Among the six primer pairs, the most efficient primer pair for genetic discrimination of these elite clones was NJFUP-poly02, which could identify 22 of the 43 elite clones directly. In conclusion, all the 43 elite clones of P. deltoides could be discriminated unambiguously based on their genotypes at the six SSR loci. The combination of all six loci gave considerable reliability and accuracy for genetic identification of these clonal accessions. Subsequently, genetic relationship of these clones was plotted by the UPGMA clustering and principle component analysis. Results of both analyses indicated that clone "C7-1" had the largest genetic distance from the other clones, followed by clone "C51-1" and a subgroup comprising clone "C100-3" and "C62-3". These clones are proposed to be possibly more affected by the inter-specific gene introgression.Key words: Populus deltoides, clone identification, SSR fingerprinting, UPGMA cluster, PCA plot.Résumé : Dans cette étude, 43 clones élite de Populus deltoides ont été marqués avec six paires d'amorces SSR par électrophorèse capillaire au moyen d'un appareil ABI 3730. D'après la cartographie peptidique, la technique aboutit à 62 bandes polymorphes comprenant en moyenne 10 allèles par locus, le nombre d'allèles amplifiés dans chaque amorce variant de 5 (NJFUP-poly10) à 16 (NJFUP-poly07) chez les clones. La valeur discriminatoire des paires d'amorces variait de 0,80 à 0,94, pour une moyenne de 0,89. Sur les six paires d'amorces, celle permettant la meilleure discrimination génétique des clones élite était NJFUP-poly02, puisqu'elle a permis d'identifier directement 22 clones sur les 43. En conclusion, on est parvenu à distinguer sans ambiguïté les 43 clones élite de P. deltoides d'après leur génotype aux six locus SSR. Combiner les six locus rend l'identification génétique extrêmement fiable et précise pour ces obtentions, issues du clonage. Par la suite, les liens génétiques entre les clones ont été tracés après regroupement par la méthode des moyennes par paire non pondérée (UPGMA) et l'analyse en composantes principales. Dans les deux cas, les résultats indiquent que le clone C7-1 est génétiquement le plus éloigné des autres. Suivaient le clone C51-1 et un sous-groupe composé des clones C100-3 et C62-3. Il se peut que ces clones soient plus affectés par l'introgression des gènes interspécifiques. [Traduit par la Rédaction]

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Characterization of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes

Cited by 29 publications

References 23 publications

Multilocus genetic structure, characterization, and relationships of Populus × canadensis cultivars

Multilocus genetic structure, characterization, and relationships of Populus × canadensis cultivars

ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genusPopulus

Genetic identification of 43 elite clonal accessions ofPopulus deltoidesby SSR fingerprinting

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