ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genus<i>Populus</i>

Gao, Jianming; Zhang, Shougong; Liwang, QI; Zhang, Yong; Wang, Chunguo; Song, Wenqin

doi:10.1051/forest:2006031

Cited by 16 publications

(9 citation statements)

References 30 publications

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“…Lu et al (2006) used ISSR primers to study seven natural populations of Populus cathayana (161 genotypes) and reported 98.7% polymorphism. In another study, ISSR marker polymorphism was a little lower (84%) among 28 genotypes of various Populus species (Jianming et al, 2006). These various studies confirm the high genetic diversity of Salix and Populus species.…”

Section: Discussionsupporting

confidence: 58%

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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This study used ISSR markers to assess the genetic diversity of a collection of 15 genotypes of Salix purpurea and 6 interspecific hybrids, employing 40 of 60 tested ISSR primers generating polymorphic amplification products. The PCR-ISSR method was adapted for S. purpurea by optimizing the annealing temperature for each primer. The polymorphism index of ISSR amplification products was 91.8% for all studied genotypes and 70.4% for S. purpurea genotypes. Nei's genetic identity statistics ranged from 0.538 to 0.958. Nei's genetic distance values were used to build a dendrogram (UPGMA) for the investigated genotypes. The dendrogram shows five clusters, and principal coordinate analysis yielded nearly the same genetic relationships among the studied genotypes. The results confirm the usefulness of ISSR markers for determining genetic diversity in S. purpurea.

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Section: Discussionsupporting

confidence: 58%

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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“…DNA based molecular markers have been identified as powerful tools in assessment of genetic relationship among rice varieties due to their highly informative nature in revealing polymorphism compared to other markers (Neeraja et al 2006;Ashfaq and Khan 2012). The high number of polymorphic loci that can be otained through SSR markers has empowered them over AFLP markers and enables them to provide more specific informations (Jianming et al 2006;Wang et al 2013). However, the results from the study carried out by Spada et al (2004) shows that AFLP and SSR markers have been successfully used to analyse the genetic diversity of two varieties of Italian rice.…”

Section: Discussionmentioning

confidence: 99%

The power of microsatellite markers and AFLPs in revealing the genetic diversity of Hashemi aromatic rice from Iran

Tarang

Gashti

2016

Journal of Integrative Agriculture

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Hashemi, a popular aromatic rice among Iranians, is famous for its fragrance and taste. Such features are major reasons for its higher price compared to non-aromatic varieties available in Iran. Therefore, the knowledge of genetic diversity of this profitable crop is a fundamental ineterst for plant breeders in future breeding programs. In the present research, genetic diversity among 35 genotypes of Hashemi aromatic rice (Oryza sativa L.) from Guilan and Mazandaran provinces of Iran was estimated using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers. Out of 21 SSR and 12 EcoRI-MseI AFLP marker combinations, only 16 SSRs and 10 AFLPs exhibited polymorphic pattern while others were monomorphic. The 10 AFLP primer combinations produced a total of 142 of bands and 20 were polymorphic (14.08%). Moreover, 40 out of 47 bands were amplified with 16 SSR markers showed polymorphism (85.1%). The average number of alleles identified by SSR primers was 2.56 alleles per locus with a range of 2 to 4. The average value of polymorphic information content (PIC) was 0.393 and 0.468 for AFLP and SSR markers respectively. However, the genetic similarity values ranged from 0.26 to 1 for SSRs and 0.21 to 1 for AFLPs. Later, a UPGMA dendrogram was generated and genotypes were clustered into four groups with SSRs at similarity coefficient of 0.55 while AFLPs clustered them into six groups at similarity coefficient of 0.41. Cluster analysis revealed a narrow genetic diversity and low correlation between geographical differentiation and genetic distance within cultivars.

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“…A great amount of useful information for genetic characterization and identification of poplars has accumulated, coming from the use of both allozyme polymorphism [28,30] or DNA markers: RAPDs [4,23,29,31] AFLPs, [1,6,8,15,40], microsatellites (SSRs) [3,6,10,14,30] and ISSRs [20].…”

Section: Introductionmentioning

confidence: 99%

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

et al. 2008

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-In order to protect the legitimate interests of the poplar breeders, growers and industries, we have developed a reliable and affordable certification tool for commercial poplars in Spain. We have carried out a genetic description of the 28 commercial clones of the current Spanish National Catalogue using 10 microsatellite molecular markers (SSR). The results clearly show that in the Spanish National Catalogue there are labelling mistakes including synonymies, supported by a combined probability of identity (PI) of 1.18 × 10 −9 . We have also demonstrate that it is possible to identify all the different clones (genotypes) with only three microsatellites, that show a combined probability of identity of 2.4 × 10 −4 , and we have optimised the protocols to reduce the time and the economical costs of the analysis. This SSR-based tool could be incorporated in the current certification protocols, to avoid duplication of accessions and identification mistakes.commercial poplar clone / microsatellite / identification / Spanish national catalogue Résumé -Développement de marqueurs microsatellites pour l'identification et la certification des clones commerciaux de peuplier en Espagne. Dans le but de protéger les intérêts légitimes des cultivateurs, des sélectionneurs et des industriels de la populiculture, nous avons mis au point un outil de certification fiable et accessible pour les clones de peupliers commerciaux en Espagne. Nous avons fait une description génétique des 28 clones commerciaux de l'actuel catalogue national espagnol en utilisant 10 marqueurs moléculaires microsatellites (SSR). Les résultats obtenus indiquent clairement que ce catalogue contient des erreurs d'identification, dont des clones synonymes, soutenues par une probabilité combinée d'identité (PI) de 1.18 × 10 −9 . Nous avons également démontré qu'il est possible d'identifier tous les génotypes différents avec 3 microsatellites, qui montrent une probabilité combinée d'identité de 2.4 × 10 −4 , et nous avons optimisé les protocoles afin de réduire le temps et le coût de l'analyse. Cet outil fondé sur les SSR pourrait être intégré dans les protocoles actuels de certification afin d'éviter la duplication des matériaux et les erreurs d'identification.clone commercial de peuplier / microsatellite / identification / catalogue national espagnol

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ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genusPopulus

Cited by 16 publications

References 30 publications

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

The power of microsatellite markers and AFLPs in revealing the genetic diversity of Hashemi aromatic rice from Iran

Développement de marqueurs microsatellites pour l’identification et la certification des clones commerciaux de peuplier en Espagne

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