Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Abstract

This study used ISSR markers to assess the genetic diversity of a collection of 15 genotypes of Salix purpurea and 6 interspecific hybrids, employing 40 of 60 tested ISSR primers generating polymorphic amplification products. The PCR-ISSR method was adapted for S. purpurea by optimizing the annealing temperature for each primer. The polymorphism index of ISSR amplification products was 91.8% for all studied genotypes and 70.4% for S. purpurea genotypes. Nei's genetic identity statistics ranged from 0.538 to 0.958. Nei's genetic distance values were used to build a dendrogram (UPGMA) for the investigated genotypes. The dendrogram shows five clusters, and principal coordinate analysis yielded nearly the same genetic relationships among the studied genotypes. The results confirm the usefulness of ISSR markers for determining genetic diversity in S. purpurea.

Genetic Diversity and Genetic Relationships of Purple Willow (Salix purpurea L.) from Natural Locations

“…However, genetic diversity was lower in Sweden and many genotypes were genetically identical; therefore, the observed results were attributed to artificial introduction of the species to Sweden [ 50 ]. With regard to S. purpurea , our study confirms the high usefulness of AFLPs, RAPDs and ISSRs in genetic diversity analyses of natural locations [ 20 , 22 , 23 , 43 ].…”

“…In our study, the genetic diversity of S. purpurea ( uHe = 0.179; Table 1 ) was high despite lower than reported by other authors, but those analyses either relied on different marker systems [ 43 ] or did not include natural locations of S. purpurea [ 20 , 22 , 23 ]. Genetic differentiation ( Φ ST = 0.212; Table 1 ) was also slightly lower than expected for plant species with comparable life-history traits (long-lived perennials, outbreeding, wind dispersal: Φ ST ≈ 0.25) [ 51 ].…”

“…This goal can be accomplished with the use of DNA markers, which are the main diagnostic tools in modern plant breeding. DNA markers are valuable tools for S. purpurea studies, inter alia to identify species of Salix [ 17 , 18 , 19 ], genotypes of S. purpurea [ 20 , 21 ] and their genetic diversity [ 20 , 22 , 23 ]. They facilitate and support structural analyses of genomes, genetic mapping, identification of quantitative trait loci (QTL), marker-assisted selection (MAS) and sequencing of S. purpurea [ 24 , 25 , 26 ].…”

The analysis of genetic diversity in willow (Salix spp.) by ISSR markers

“…In our study, effective alleles accounted for 78.48% of total number of alleles, which obtained higher than the value reported by Przyborowski and Sulima (2010) analyzing Salix viminalis genotypes (76.3%) based on RAPD marker. However, ten selected markers generated 93.67% polymorphism and 145 polymorphic bands, which appeared to be higher than the value reported earlier by Sulima and Przyborowski (2013) among 15 genotypes of S. purpurea (70.40%) employing 40 ISSR primers. The difference in polymorphic value reflects the diverse material and the inadequate number of ISSR markers in present study.…”

Supplementary material 2 from: Urbaniak J, Kwiatkowski P, Pawlikowski P (2021) Genetic diversity of Salix lapponum populations in Central Europe. PhytoKeys 184: 83-101. https://doi.org/10.3897/phytokeys.184.71641

“…These markers have some limitations in use and are less effective than, for example AFLP, but are still used in research. Prior to the study, about 80 ISSR primers were checked for their usefulness in determining population differentiation in S. lapponum, based on the experience of Sulima and Przyborowski (2013). A total of twelve primers: ISSR: 2, 3,4,5,91,92,93,94,95,137,139,142 showed a satisfactory amplification and generated an acceptable number of polymorphic bands (Suppl.…”