Multilayer scaffold of electrospun PLA–PCL–collagen nanofibers as a dural substitute

Wang, Yufei; Guo, Hongfeng; Ying, Dajun

doi:10.1002/jbm.b.32953

Cited by 61 publications

(33 citation statements)

References 57 publications

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“…The basic mechanical performance of the biomimetic patch showed that among transverse and longitudinal directions, the tensile strength ranged from 2.8 to 4.3 Mpa and showed no significant difference ( P = 0.4716, n = 8). Combining the transverse and longitudinal results, the average tensile strength was 4.14 ± 0.18 Mpa ( n = 8), the average stretching elongation was 60.5 ± 13.2% ( n = 8), and the average stitch tear load was 3.71 ± 0.46 N ( n = 8), with all samples having a stitch tear load of >1 N. Compared with the low mechanical properties of collagen matrix substitutes , the tensile strength of this biomimetic patch can be sutured to the dogs' dural mater easily without CSF leakage.…”

Section: Resultsmentioning

confidence: 92%

“…To avoid previously encountered complications, Medprin Biotech GmbH developed a biodegradable nonwoven substitute composed of Poly‐L‐lactide (PLLA) fibers with open, highly interconnected pores to create the unique 3D structure that facilitates cell infiltration and tissue generation, and is gradually absorbed by the body. Besides, the PLLA has been shown to be fully compatible with dural tissue and can also reduce tissue adhesion . The objective of this study was to test the safety and effectiveness of this biomimetic patch for dural repair.…”

mentioning

confidence: 99%

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A New Absorbable Synthetic Substitute With Biomimetic Design for Dural Tissue Repair

Shi

Yuan

et al. 2015

Artificial Organs

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Dural repair products are evolving from animal tissue-derived materials to synthetic materials as well as from inert to absorbable features; most of them lack functional and structural characteristics compared with the natural dura mater. In the present study, we evaluated the properties and tissue repair performance of a new dural repair product with biomimetic design. The biomimetic patch exhibits unique three-dimensional nonwoven microfiber structure with good mechanical strength and biocompatibility. The animal study showed that the biomimetic patch and commercially synthetic material group presented new subdural regeneration at 90 days, with low level inflammatory response and minimal to no adhesion formation detected at each stage. In the biological material group, no new subdural regeneration was observed and severe adhesion between the implant and the cortex occurred at each stage. In clinical case study, there was no cerebrospinal fluid leakage, and all the postoperation observations were normal. The biomimetic structure and proper rate of degradation of the new absorbable dura substitute can guide the meaningful reconstruction of the dura mater, which may provide a novel approach for dural defect repair.

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Section: Resultsmentioning

confidence: 92%

mentioning

confidence: 99%

A New Absorbable Synthetic Substitute With Biomimetic Design for Dural Tissue Repair

Shi

Yuan

et al. 2015

Artificial Organs

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“…Electrospinning which uses electrical forces to generate polymeric fibers with nano-/micro-sized diameters is an effective, cost efficient, and easily applied technique [23,24]. Electrospun fibers have been successfully used in drug delivery, tissue engineering, and other biomedical applications due to their highly porous structures, high surface area to volume ratio, potential biocompatibility, and high functionality [25][26][27]. In the literature, although there are limited studies based on coating of Ti implants with electrospun fibers, to the best of our knowledge, no investigation has been demonstrated for PEO nanofiber-coated implant surfaces in terms of cellular/bacterial adhesion.…”

Section: Introductionmentioning

confidence: 99%

Anticellular PEO coatings on titanium surfaces by sequential electrospinning and crosslinking processes

2019

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Titanium implants having some superior properties for tissue replacements have been widely used in biomedical fields. However, the implants are vulnerable to bacterial attacks and therefore they must be modified. In this study, two kinds of titanium surfaces, bare (Ti-b) and sandpapered (Ti-p) titanium, were used and electrospinning method was utilized for coating them with poly(ethylene oxide) (PEO) nanofibers with average diameter of 180 nm. In order to obtain insoluble coating, the PEO nanofibers were crosslinked by UV-initiating and crosslinking agent, pentaerythritol triacrylate (PETA), in the presence of UV irradiation at 366nm wavelength. Fibroblastic MC3T3-E1 preosteoblasts and S. epidermidis bacteria were cultured on these surfaces to investigate their attachment and proliferation behavior. The preosteoblasts cultured on Tip exhibited better initial adhesion than that of Ti-b at the end of the 4 h of incubation period, which reveals the importance of surface roughness. The bacteria adhered and colonized on Ti-b surfaces at the end of the 24 h of incubation. In contrast, Ti surfaces modified by the PEO nanofibers inhibited cellular and bacterial attachment significantly. This study discloses that electrospinning and subsequent crosslinking of PEO can be evaluated as an effective approach for creating anticellular coatings for Ti implants.

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“…In another set of experiments, we studied the adhesion and growth of human keratinocytes on nanofibrous membranes made of poly-ε-caprolactone (PCL) and its copolymer with PLA (PLA/PCL, ratio 70:30). PCL and PLA/PCL copolymers have been experimentally used for vascular tissue engineering, particularly for replacement of small caliber blood vessels [31,32], neural tissue engineering, specifically for generation of conductive sheaths for neurite outgrowth [104], for substituting the dura mater [105], and also for bone tissue engineering in order to mimic hemi-osteons and to control the spatial organization of osteoblasts [106]. These applications of PCL and PLA/PCL were enabled, among others, by suitable mechanical properties of these polymers, particularly in case of PLA/PCL.…”

Section: Nanofibers In Skin Tissue Engineeringmentioning

confidence: 99%

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková¹,

Bačáková²,

Pajorová³

et al. 2016

Nanofiber Research - Reaching New Heights

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Nanofibers are promising cell carriers for tissue engineering of a variety of tissues and organs in the human organism. They have been experimentally used for reconstruction of tissues of cardiovascular, respiratory, digestive, urinary, nervous and musculoskeletal systems. Nanofibers are also promising for drug and gene delivery, construction of biosensors and biostimulators, and wound dressings. Nanofibers can be created from a wide range of natural polymers or synthetic biostable and biodegradable polymers. For hard tissue engineering, polymeric nanofibers can be reinforced with various ceramic, metal-based or carbon-based nanoparticles, or created directly from hard materials. The nanofibrous scaffolds can be loaded with various bioactive molecules, such as growth, differentiation and angiogenic factors, or funcionalized with ligands for the cell adhesion receptors. This review also includes our experience in skin tissue engineering using nanofibers fabricated from polycaprolactone and its copolymer with polylactide, cellulose acetate, and particularly from polylactide nanofibers modified by plasma activation and fibrin coating. In addition, we studied the interaction of human bone-derived cells with nanofibrous scaffolds loaded with hydroxyapatite or diamond nanoparticles. We also created novel nanofibers based on diamond deposition on a SiO 2 template, and tested their effects on the adhesion, viability and growth of human vascular endothelial cells.

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Multilayer scaffold of electrospun PLA–PCL–collagen nanofibers as a dural substitute

Cited by 61 publications

References 57 publications

A New Absorbable Synthetic Substitute With Biomimetic Design for Dural Tissue Repair

A New Absorbable Synthetic Substitute With Biomimetic Design for Dural Tissue Repair

Anticellular PEO coatings on titanium surfaces by sequential electrospinning and crosslinking processes

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

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