In this study, fibrous mats were fabricated via electrospinning from solutions of polyethylene terephthalate (PET), PET/chitosan, and PET/honey at different concentrations. The effect of honey and chitosan on electrospinning process was investigated and compared. Fibers containing chitosan had a beaded or ribbon-like/branched morphology, but this morphology improved in the presence of honey. The diameter of electrospun fibers decreased with an increased ratio of honey in PET solution. In addition, fiber deposition area in the collector increased by increasing the honey content. PET/chitosan and PET/honey fibrous mats reached an equilibrium water content in 15 min and their water uptake capacities, which are important for exudating wounds, were found in the range of 280-430% on dry basis. Cytotoxicity evaluation demonstrated that fibers exhibited no cytotoxic activity. This study discloses that PET fibrous mats especially electrospun in the presence of honey could be proposed as potential wound dressing materials owing to their improved processing abilities besides their suitable structural properties.

Titanium implants having some superior properties for tissue replacements have been widely used in biomedical fields. However, the implants are vulnerable to bacterial attacks and therefore they must be modified. In this study, two kinds of titanium surfaces, bare (Ti-b) and sandpapered (Ti-p) titanium, were used and electrospinning method was utilized for coating them with poly(ethylene oxide) (PEO) nanofibers with average diameter of 180 nm. In order to obtain insoluble coating, the PEO nanofibers were crosslinked by UV-initiating and crosslinking agent, pentaerythritol triacrylate (PETA), in the presence of UV irradiation at 366nm wavelength. Fibroblastic MC3T3-E1 preosteoblasts and S. epidermidis bacteria were cultured on these surfaces to investigate their attachment and proliferation behavior. The preosteoblasts cultured on Tip exhibited better initial adhesion than that of Ti-b at the end of the 4 h of incubation period, which reveals the importance of surface roughness. The bacteria adhered and colonized on Ti-b surfaces at the end of the 24 h of incubation. In contrast, Ti surfaces modified by the PEO nanofibers inhibited cellular and bacterial attachment significantly. This study discloses that electrospinning and subsequent crosslinking of PEO can be evaluated as an effective approach for creating anticellular coatings for Ti implants.