Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková, Lucie; Bačáková, Markéta; Pajorová, Júlia; RadmilaKudlackova,; Staňková, Lubica; Filová, Elena; Musı́lková, Jana; StepanPotocky,; Kromka, Alexander

doi:10.5772/63707

Cited by 5 publications

(7 citation statements)

References 121 publications

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“…They are considered regulators of cell differentiation and maintenance of the phenotype of implanted cells, 21,22 while through their negatively charged chains they can act as reservoirs for GFs and chemokines that finely control their release and therefore cell signaling 23 . Among GAGs, HS, heparin, and CS have a well‐documented use as components of nanofibers for skin tissue engineering applications 22 . Heparin has the highest negative charge density of any biological macromolecule providing high affinity for growth factors (GFs), such as fibroblast growth factors and other factors important for wound healing 24,25 .…”

Section: Introductionmentioning

confidence: 99%

“…GAGs are widely used in tissue engineering constructs to recapitulate the ECM. They are considered regulators of cell differentiation and maintenance of the phenotype of implanted cells, 21,22 while through their negatively charged chains they can act as reservoirs for GFs and chemokines that finely control their release and therefore cell signaling 23 . Among GAGs, HS, heparin, and CS have a well‐documented use as components of nanofibers for skin tissue engineering applications 22 .…”

Section: Introductionmentioning

confidence: 99%

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Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth

Michopoulou¹,

Koliakou

Terzopoulou

et al. 2021

J Biomedical Materials Res

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Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications. This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments. Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen‐based porous materials to serve as scaffolds for the three‐dimensional expansion of freshly isolated papillary and/or reticular fibroblasts. The scaffolds, composed of either collagen I or collagen I and III mixtures, were prepared by lyophilization. Pore size and hydrolytic stability were controlled by crosslinking with 1‐ethyl‐3‐(3‐dimethyl aminopropyl) carbodiimide (EDC) and N‐hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin. The evaluation of the resultant “papillary” and “reticular” dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections. Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation. The presence of collagen III seemed to have some positive but non‐specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation. Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth

Michopoulou¹,

Koliakou

Terzopoulou

et al. 2021

J Biomedical Materials Res

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“…Cold plasma treatment of polymer nanofibers was greatly described as a clean and environmentally friendly technique to improve nanofibers biocompatibility for use in biomedical and tissue engineering domains. − The importance of surface chemistry on biocompatibility and cell behavior was largely described in the literature. In this sense, Thevenot et al summarized the effect of classical chemical functions grafted onto the surface of biomaterials on their biological properties .…”

Section: Introductionmentioning

confidence: 99%

Effect of Cold Plasma Treatment on Electrospun Nanofibers Properties: A Review

Dufay

Jimenez

Degoutin

2020

ACS Appl. Bio Mater.

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Electrospinning of polymer materials is a widely used technique, providing nanofibrous mats with high surface to volume ratio and porosity, which show a great interest in many fields such as biomedical applications. However, nanofibers generally present some weakness including low mechanical resistance, poor bioactivity, or lower biocompatibility. Among the surface modification techniques described in the literature, cold plasma treatment may prevent these drawbacks and especially improve properties of electrospun nanofibers. Therefore, this review lays out the state-of-the-art of cold plasma treatment of nanofibers and, especially, its effect on the biocompatibility improvement, the immobilization/adsorption of molecules of interest, the surface grafting/cross-linking, and the use of the modified nanofibers in biomedical applications. In particular, this literature review demonstrates the positive effect of cold plasma treatment onto the mechanical, biological, or chemical properties of nanofibers. Future investigations should go further on the effects of the gas type but also on the possibility of coupling cold plasma treatment with other surface modification techniques.

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“…Nanofibrous scaffolds are one of the most promising materials for skin tissue engineering and wound dressing, because they resemble nanoarchitecture of the native extracellular matrix (for a review, see [1]). Therefore, they can serve as suitable carriers of cells for tissue engineering and also as suitable wound dressings, which are able to protect the wound from external harmful effects, mainly Scanning electron microscopy of nanofibrous layers produced by wire needleless electrospinning using different solvents, namely 12 wt% of CA in acetone/DMAC (9:1) (left) or 14 wt% of CA in 95% AA (right).…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Cited by 5 publications

References 121 publications

Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth

Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth

Effect of Cold Plasma Treatment on Electrospun Nanofibers Properties: A Review

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

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