MtDNA Barcode Identification of Finfish Larvae from Vellar Estuary, Tamilnadu, India

Thirumaraiselvi, Ramakrishnan; DAS, Sourin; Ramanadevi, Vellaichamy; Thangaraj, M.

doi:10.15835/nsb719478

Cited by 6 publications

(1 citation statement)

References 17 publications

(11 reference statements)

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“…The low genetic distance of 0.002 was reported by Nuryanto et al (2017) on two morphotypes larvae, which was suggested to belong to single species. Additional earlier studies also proved that individuals from single species had low genetic distances (Thirumaraiselvi et al, 2015;Hubert et al, 2012), even among conspecific specimens collected from geographically separated populations (Sachithanandam et al, 2012). Higher genetic distance among individuals from single species was reported in other groups of animals, such as in thrips (Kurniawaty et al, 2016), andin Lepidoptera (deWaard et al, 2011).…”

Section: Resultsmentioning

confidence: 82%

Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java

2020

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The existence of mole crabs had been reported from the southern coast of Central Java. However, no mole crab data was available from the northern coast Central Java. This study aimed to figure out the existence of mole crabs in the northern coast of Central Java as revealed from the cytochrome c oxidase 1 (COI) barcoding. Mole crabs samples collected in Sendang Sikucing Beach Kendal. Taxonomic status of the samples was inferred from sequences similarity test using basic local alignment search tool to conspecific sequence deposited in GenBank. Pairwise genetic distances were calculated based on the Kimura 2-parameter model. A phylogenetic tree had reconstructed in molecular evolution genetic analysis (MEGA) software based on neighbor-joining algorithm. Branching reliability was obtained from 1000 bootstraps replication while branching polarity was obtained from the out-group comparison. The mole crab samples from the northern coast of Central Java had unambiguously identified as Emerita emeritus based on high sequences similarities (98.27%-99.70%), low genetic distances (0.002-0.005), and their monophyly with Emerita emeritus (KR047035) in GenBank. Pairwise analysis among each possibility pair of samples had genetic distances ranged between 0.000 and 0.005, indicated that all sample belong to single species. The data provide the first record of the existence of Emerita emeritus in the northern coast of Central Java. Our data on the existence of E. emeritus in the northern coast of Central Java is among essentials information as a scientific basis in creating policy for the management of sustainable use of the mole crabs in the areas.

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Section: Resultsmentioning

confidence: 82%

Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java

2020

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Identifying fish eggs and larvae: from classic methodologies to DNA metabarcoding

et al. 2022

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DNA barcoding and taxonomic validation ofCaranxspp. larvae

et al. 2021

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Of the five nominal species in the genus Caranx Lacepède 1801 distributed throughout the Eastern Central Pacific, Caranx caballus and Caranx sexfasciatus are the only two that have formal fish larval descriptions based on diagnostic characteristics (morphology, meristics and pigmentation). In this study, the diagnostic characteristics of three Caranx species larvae were validated using DNA barcoding analysis cytochrome c oxidase subunit I (COI; 651 bp). For the first time, the morphological taxonomic assignation of C. caballus fish larvae was confirmed using COI gene partial sequences of adults, with a genetic similarity between 99.8–100%. However, molecular evidence demonstrated that fish larvae previously described as C. sexfasciatus had high genetic similarity (99.7–100%) and low genetic distance (<1%) to Caranx caninus adults. An undescribed larval morphotype collected in the present study genetically matched (100%) with COI sequences of C. sexfasciatus adults. The diagnostic characteristics of this new morphotype were a lack of pigmentation in the supraoccipital crest, over the gut, and at the terminal region of the gut. The combination of diagnostic characteristics and DNA barcoding evidence allowed the discrimination and validation of C. caballus, C. caninus and C. sexfasciatus larvae. The diagnostic characteristics and COI sequences of Caranx lugubris and Caranx melampygus larvae, which are also distributed in the Eastern Central Pacific, remain to be investigated.

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MtDNA Barcode Identification of Finfish Larvae from Vellar Estuary, Tamilnadu, India

Cited by 6 publications

References 17 publications

Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java

Molecular Barcoding Reveal the Existence of Mole Crabs Emerita emeritus in North Coast of Central Java

Identifying fish eggs and larvae: from classic methodologies to DNA metabarcoding

DNA barcoding and taxonomic validation ofCaranxspp. larvae

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