Planktonic larvae were captured in the shallow Vellar estuary, Tamilnadu, India, during January to June 2013 using zooplankton net. Larvae were identified to the species level by comparison with Genbank data base using MtDNA sequence data. Further analysis showed that there is no sequence variation within-species, whereas between-species variation was more than 25%, supporting the suitability for species identification. The highest genetic distance was observed between A. nigropunctatus and M. cephalus (0.434) and the lowest genetic distance was observed between T. jarbua and S. commersoni (0.256). Given the current worldwide interest in DNA barcoding and species identification using MtDNA gene marker (CO1), it was confirmed the efficacy of the Fish-F1 and -R1 primer set, which gave the possibility for identification of finfish larvae at species level.
Morphometric character analyses and RAPD was used to discriminate and ratify the status of three populations of Indian salmon, Polydactylus plebeius along the coromandel coast of India. Morphometric analyses showed a clear pattern of differentiation between the stocks and revealed the discreteness of two groups, southern stock (Pazhayar) and northern stock (Cuddalore). The univariate analysis of variance showed significant differences between means of the samples for most morphometric descriptors. A total of 1077 scorable bands were produced using all ten arbitrary primers in three populations. An un-weighted pair-group method with arithmetic mean (UPGMA) dendrogram was constructed based on genetic values to show the genetic relationship among the three populations. The genetic diversity (H) of P. plebeius in Cuddalore was more (0.0733 ± 0.0648) than Pazhayar (0.0609 ± 0.0416) and Vellar (0.0613 ± 0.0344) populations. All the three populations had significantly (p
Eleutheronema tetradactylum is an important commercial fish species exposed to intense exploitation both in Southeast Asian countries and Northern parts of Australia. Research on the population structure of E. tetradactylum in these coastal waters is substantial in order to ensure sustainable use and appropriate resource management. In this study, genetic variation, diversity and population structure of E. tetradactylum among four FAO fishing areas, along South Asian countries, were evaluated using cytochrome c oxidase subunit I (COI) gene. Totally 30 sequences of COI gene were collected from four FAO fishing areas. Among these 30 individuals, 18 distinct haplotypes were defined. High levels of haplotype diversity (hd = 0.952 ± 0.096) and nucleotide diversity (π = 0.01536 ± 0.00312) were observed in the population within the Bay of Bengal. No haplotype and nucleotide diversity were observed in South China Sea population. Hierarchical analysis of molecular variance (AMOVA) indicated that whereas 0.81% of the genetic variation occurred within the populations, 7.09% occurred among populations. Significant genealogical branches were recognized in North Australian populations (one clade), South China Sea populations (one clade), Arabian Sea and Bay of Bengal populations (one clade on the neighbor-joining tree). These results suggested that E. tetradactylum populations in FAO fishing areas 51, 57 and 61 have developed different genetic structures. Tests of neutral evolution and mismatch distribution suggest that a population growth of E. tetradactylum may take place in these fishing areas.
Planktonic larvae were captured in the shallow Vellar estuary, Tamilnadu, India, during January to June 2013 using zooplankton net. Larvae were identified to the species level by comparison with Genbank data base using MtDNA sequence data. Further analysis showed that there is no sequence variation within-species, whereas between-species variation was more than 25%, supporting the suitability for species identification. The highest genetic distance was observed between A. nigropunctatus and M. cephalus (0.434) and the lowest genetic distance was observed between T. jarbua and S. commersoni (0.256). Given the current worldwide interest in DNA barcoding and species identification using MtDNA gene marker (CO1), it was confirmed the efficacy of the Fish-F1 and -R1 primer set, which gave the possibility for identification of finfish larvae at species level.
Eleutheronema tetradactylum is an important commercial fish species exposed to intense exploitation both in Southeast Asian countries and Northern parts of Australia. Research on the population structure of E. tetradactylum in these coastal waters is substantial in order to ensure sustainable use and appropriate resource management. In this study, genetic variation, diversity and population structure of E. tetradactylum among four FAO fishing areas, along South Asian countries, were evaluated using cytochrome c oxidase subunit I (COI) gene. Totally 30 sequences of COI gene were collected from four FAO fishing areas. Among these 30 individuals, 18 distinct haplotypes were defined. High levels of haplotype diversity (hd = 0.952 ± 0.096) and nucleotide diversity (π = 0.01536 ± 0.00312) were observed in the population within the Bay of Bengal. No haplotype and nucleotide diversity were observed in South China Sea population. Hierarchical analysis of molecular variance (AMOVA) indicated that 0.81% of the genetic variation occurred within the populations, while 7.09% variation occurred among populations. Significant genealogical branches were recognized in North Australian populations (one clade), South China Sea populations (one clade), Arabian Sea and Bay of Bengal populations (one clade) on the neighbor-joining tree. These results suggested that E. tetradactylum populations in FAO fishing areas 51, 57 and 61 have developed different genetic structures. Tests of neutral evolution and mismatch distribution suggest that a population growth of E. tetradactylum may take place in these fishing areas.
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