Morphological Aspects of the Embryonic Development of the TMJ in Rats (Rattus norvegicus albinus) Treated With Fluoxetine

Abstract

The TMJ has been to the Dental community a key point in the search of knowledge, being it part of the temporomandibular joint complex and of the estomatognathic system which are in charge of the mastication, speech, swallowing, as well as participation in breathing and taste perception. For the majority of the women in serious state of depression, which do not respond psychotherapeutic treatment, pharmacological treatment it's applied. The antidepressants serotonin selective reuptake inhibitors (SSRIs) are the most recommended in these cases. The teratogenic effect of the SSRIs is considered controversial, studies done with women who used these drugs during the pregnancy showed that the respiratory and central nervous systems are the most affected, was also recorded a deficit of body growth and the decrease of the encephalon and skull measures. In the present study, our goal was to assess whether the administration of Fluoxetine during the pregnancy modified the embryology and morphology of the TMJ of rats. For that, 16 Wistar female rats from the Nutrition Department of the UFPE vivarium were selected; 8 for the control group, which received daily 0.9% of saline in subcutaneous dose of 10ml/g, with schedules previously established after daily weighing and 8 for the experimental one that were treated with fluoxetine hydrochloride with the dose of 10mg/Kg in a volume 10ml/g of weight, were injected subcutaneously with the same standards established for the control group. It was observed, with this dose that the embryological development of the TMJ, especially of the mandibular condyle, does not present any difference between the degree of maturation of the tissue that forms the TMJ, especially of the condyle between the treated and control groups.

Fluoxetine effects on periodontogenesis: histomorphometrical and immunohistochemical analyses in rats

“…Studies previously conducted by our research group did not reveal alterations in the formation and morphology of the temporomandibular joint (TMJ) 7 and tooth enamel of rat pups treated with fluoxetine (10 mg/kg) during pregnancy 29 . Nevertheless, the administration of 20 mg/kg fluoxetine during rat pregnancy and lactation modified the jawbone mass of the pups 8 .…”

Effects of treatment with fluoxetine on mandibular development: A morphological study in rats

“…Previous studies have been conducted using the dosage of 10 mg/kg via the subcutaneous route, to investigate possible alterations in the temporomandibular joint and tooth enamel, and no evidence was found (Cavalcanti et al, 2009;Silva et al, 2010). Therefore, in the present study, a dose of 20 mg/kg via the oral route was used.…”

Temporomandibular Joint Histology

“…The temporomandibular joint is a collection of anatomical structures that, with the participation of special groups of muscles, allows the jaw to perform various movements during chewing [15]. Classically, it has been described as a bilateral synovial joint of the ginglymo-arthroid type, which allows rotation and displacement of the mandibular condyle [17,18] in addition to opening, closing, protrusion, retrusion and laterality movements of the mandible [19,20].…”

“…Subsequently, these were immersed in a bath (Boekel Scientific; USA) at 65°C, placed on slides, and stored between 35 and 38°C for a minimum of 24 hours. H&E staining involved twenty-three steps lasting approximately one hour [12][13]15]. The In this study, adult female Wistar strain rats (Rattus norvegicus) sourced from the Intermediate Laboratory of Preclinical Research at Valle University were utilized.…”