Fluoxetine effects on periodontogenesis: histomorphometrical and immunohistochemical analyses in rats

Abstract

Reports have indicated that serotonin plays an important role in cell migration and differentiation during the organogenesis of several tissues, including the oral types. Administration of selective serotonin reuptake inhibitor (SSRI) drugs during pregnancy could affect the delivery of serotonin to embryonic tissues altering its development. Objective This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups.Material and Methods Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope.Results The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers.Conclusion These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation.

Histological, immunohistochemical and radiographic evaluation of Amitriptyline administration on the periodontium of albino rats (An experimental study)

“…Absence of cementoblastic lining with some empty cementocytes lacunae and others with pyknotic nuclei was seen. These findings agree with Regueira et al, (2017) who correlated between SSRIs and periodontal cells. They suggested that periodontal cells are sensitive to Fluoxentine “a SSRIs” when taken with a dose 20 mg/kg during pregnancy and lactation and they reported reduction in cementoblasts, fibroblasts and osteoblasts quantities.…”

Microscopic Changes in Rat’s Gingiva Under Conditions of Experimental Periodontitis and the Use of Mesenchymal Stem Cells

“…Генералізований пародонтит залишаться однією з гострих проблем сучасної стоматології, й актуальність зумовлена високою поширеністю хвороби за не завжди задовільного ефекту численних засобів і методів, які пропонують для її лікування [4,5]. Саме це зумовило підвищену увагу науковців до пошуку нових патогенетичних методів терапії запальних і запально-деструктивних процесів у тканинах пародонтального комплексу, зокрема ясен [6][7][8][9][10]. Клітинна терапія -інноваційний, надзвичайно перспективний метод лікування хвороб і травм, що дозволяє відновити пошкоджені тканини організму та органи за допомогою трансплантації здорових нових клітин -стовбурових клітин.…”

Histological and histometric aspects of radicular dentinogenesis in upper first molars of fluoxetine treated rats

“…Studies have shown that changes in body's 5-HT levels during embryogenesis caused changes in the growth and development of many tissues, especially in the craniofacial region (Mendes-da- Silva et al, 2002;Santiago et al, 2013;Correia-Leite de Marcelos et al, 2015;Regueira et al, 2017). Reports from Lauder et al (2000) showed that embryos exposed to antagonists of 5-HT2 receptors exhibited different embryological malformations depending on the dose and selectivity of the used antagonist.…”