Morphokinetics of embryos developed from oocytes matured in vitro

Canto, Mariabeatrice Dal; Novara, Paola; Coticchio, Giovanni; Renzini, Mario Mignini; Brambillasca, Fausta; Brigante, Claudio; Ponti, Elena De; Fadini, Rubens

doi:10.1007/s10815-015-0625-9

Cited by 16 publications

(11 citation statements)

References 33 publications

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“…Full imaging of the developmental competence of the embryos derived from IVM oocytes is an important task to ascertain feasible ways to improve the IVM technique as a clinical approach. It is clear that the final proof of oocyte developmental competence is its ability to develop a viable pregnancy (Dal Canto et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

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Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Faramarzi

Khalili

Ashourzadeh

et al. 2018

Zygote

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Currently, rescue in vitro maturation (IVM) is not a routine method in assisted reproductive treatment (ART) programmes but is a promising procedure for ART to improve IVM. The aim of this study was to compare embryo morphokinetics of germinal vesicles (GV) with metaphase II (MII) oocytes from controlled ovarian hyperstimulation (COH) cycles by timelapse photography monitoring (TLM). Morphokinetics of the same number of embryos derived from the in vivo (group I) and rescue of in vitro matured oocytes (group II) from 310 patients were analyzed and compared retrospectively. The time to form second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf) and time of two to eight discrete cells (t2-t8) were assessed. Abnormal cleavage patterns such as uneven blastomeres at the two-cell stage, cell fusion (Fu), trichotomous mitoses (TM), and the rates of embryo arrest were assessed. These data showed that tPB2, tPNa, tPNf, t2, t3 and t4 stages took place later in group II compared with group I (P < 0.001, P = 0.017, P < 0.001, P < 0.001, P < 0.001, P < 0.001, respectively). The rates of uneven blastomeres, Fu, TM, and embryo arrest were increased significantly in group II compared with group I (P = 0.001, P < 0.001, P = 0.003, P < 0.001, respectively). Based on the exact annotation of timing parameters and cleavage patterns, the present data agreed with the concept that rescue IVM of oocytes negatively influences embryo morphokinetics. Therefore, cautious use of embryos derived from rescue IVM of GV oocytes should be made. Zygote cambridge.org/zyg Research Article Cite this article: Faramarzi et al. (2018) Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development? Zygote 26: 430-434.

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Section: Introductionmentioning

confidence: 99%

“…Azita Faramarzi et al differences in morphokinetics time points between embryos derived from in vitro or in vivo matured oocytes (Walls et al, 2015;Dal Canto et al, 2016). Also, Roesner and colleagues showed that there were no differences in the tPB2, tPNf, t2, t3, t4 and t5 time points.…”

mentioning

confidence: 99%

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Faramarzi

Khalili

Ashourzadeh

et al. 2018

Zygote

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“…The results of current research showed that human oocytes matured in vivo or in vitro display no significant differences in terms of their spindle morphology, organelle distribution, cortical particle distribution, and mitochondrial morphology [38,39]. By observing embryos dynamically using time-lapse videos, it was confirmed that oocytes matured in vivo or in vitro showed no significant differences in terms of the morphological dynamics observed during the early development of embryos derived from these oocytes [40]. Another study has also shown that the oocytes matured in vitro and in vivo are different in terms of their organelle function, distribution, and gene expression [41].…”

Section: Clinical Application and Safety Of Ivmmentioning

confidence: 54%

Clinical Application of In Vitro Maturation of Oocytes

La¹,

Zhao²,

Wang³

2019

Embryology - Theory and Practice

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In vitro maturation (IVM) is a technique used to induce immature oocytes collected in different periods of embryonic growth. The rates vary for immature oocytes collected from different clinical sources to potentially develop into embryos and achieve live birth. As an effective treatment method, IVM can be used to treat patients with polycystic ovary syndrome (PCOS), ovarian hyperresponsiveness, and hyporesponsiveness, as well as to preserve the fertility of cancer patients. This technology has been used worldwide for the birth of thousands of healthy babies. The improvement in clinical IVM technology mainly focuses on the IVM medium and the optimization of the culture environment and operation process. At present, with the improvement in the in vitro fertilization (IVF) efficiency and culture systems, a natural cycle or mild stimulation may be more suitable for women receiving IVF treatments. A new treatment option was proposed to combine natural cycle/ mild stimulation IVF with IVM. In particular, the combination of mild stimulation IVF and IVM is not only expected to become a viable alternative to current standard treatments but may also become a potential option of first-line treatment.

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“…The authors concluded that the ultrastructure of matured oocytes from IVM cycles had similar cytoarchitecture in both vitrified-warmed and previtrified oocytes in EM studies ( 111 ) . Dal Canto et al ( 112 ) studied the morphokinetics of embryos derived from in vitro matured oocytes from hCG-primed IVM cycles. Oocytes derived from 8-12 mm follicles were categorized according to the cumulus expansion and expanded cumulus oocytes were evaluated as MII and incubated for 6 hours, whereas others were accepted as GV oocytes and incubated for 30 hours.…”

Section: Discussionmentioning

confidence: 99%

“…Oocytes derived from 8-12 mm follicles were categorized according to the cumulus expansion and expanded cumulus oocytes were evaluated as MII and incubated for 6 hours, whereas others were accepted as GV oocytes and incubated for 30 hours. The authors concluded that morphokinetic behavior of both mature and GV oocytes from IVM cycles were comparable and it was suggested that only minimal differences were present in GV and MII oocytes from IVM cycles ( 112 ) . Embryos derived from GV oocyte maturation in stimulated IVF cycles had a high arrest rates and multinucleation rates but meiotic resumption happened normally.…”

Section: Discussionmentioning

confidence: 99%

Oocyte in vitro maturation: A sytematic review

Hatırnaz¹,

Ata

Hatırnaz³

et al. 2018

tjod

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In vitro maturation (IVM) is one of the most controversial aspects of assisted reproductive technology. Although it has been studied extensively, it is still not a conventional treatment option and is accepted as an alternative treatment. However, studies have shown that IVM can be used in almost all areas where in vitro fertilization (IVF) is used and it has a strong place in fertility protection and Ovarian Hyperstimulation syndrome management. The aim of this systematic review was to address all aspects of the current knowledge of IVM treatment together with the evolution of IVM and IVF.

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Morphokinetics of embryos developed from oocytes matured in vitro

Cited by 16 publications

References 33 publications

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Clinical Application of In Vitro Maturation of Oocytes

Oocyte in vitro maturation: A sytematic review

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