Clinical Application of In Vitro Maturation of Oocytes

Abstract

In vitro maturation (IVM) is a technique used to induce immature oocytes collected in different periods of embryonic growth. The rates vary for immature oocytes collected from different clinical sources to potentially develop into embryos and achieve live birth. As an effective treatment method, IVM can be used to treat patients with polycystic ovary syndrome (PCOS), ovarian hyperresponsiveness, and hyporesponsiveness, as well as to preserve the fertility of cancer patients. This technology has been used worldwide for the birth of thousands of healthy babies. The improvement in clinical IVM technology mainly focuses on the IVM medium and the optimization of the culture environment and operation process. At present, with the improvement in the in vitro fertilization (IVF) efficiency and culture systems, a natural cycle or mild stimulation may be more suitable for women receiving IVF treatments. A new treatment option was proposed to combine natural cycle/ mild stimulation IVF with IVM. In particular, the combination of mild stimulation IVF and IVM is not only expected to become a viable alternative to current standard treatments but may also become a potential option of first-line treatment.

Preservação da fertilidade em pacientes oncológicos

“…Por fim, há também o tratamento por meio da remoção cirúrgica dos órgãos reprodutores femininos. A cirurgia pode consistir na histerectomia total, com a remoção completa do útero e preservação da função ovariana, subtotal, com a remoção apenas do corpo uterino, ou radical, com a remoção completa do útero, da porção superior da vagina e do paramétrio [18][19][20][21][22] .…”

The Effects of an In Vitro Oocyte Maturation System and Chlorogenic Acid Supplementation during Embryo Culture on the Development of Porcine Cloned Embryos Derived from Native Vietnamese Ban Pigs

“…Hormone treatment during IVM, including the duration of exposure, promotes cumulus cell expansion and stimulates oocyte maturation, influencing the fertilisation rate and embryo development [ 15 , 26 ]. In addition, under in vitro conditions, the addition of hormones during maturation helps to synchronise the development of the nucleus and cytoplasm in oocytes [ 27 ]. Funahashi and Day [ 15 ] reported in a conventional IVF system that hormone exposure during IVM for 20, 30, and 40 h significantly increased oocyte maturation rates compared to those at 0 or 2 h of hormone exposure, in which 20 h of hormone exposure improved the proportion of oocytes with male and female pronuclei (normal fertilisation).…”

An interpretable and versatile machine learning approach for oocyte phenotyping

“…Moreover, oocytes can also be extracted and frozen for fertility preservation. The success rate of oocyte in vitro maturation is typically around 70% (Kim et al, 2004), but the developmental potential of in vitro matured oocytes is much lower than for in vivo matured ones (Kim et al, 2004; La et al, 2019; Monti et al, 2017). To improve in vitro oocyte maturation protocols, we need to better decipher the parameters associated with successful oocyte development.…”

“…To improve in vitro oocyte maturation protocols, we need to better decipher the parameters associated with successful oocyte development. Several characteristics correlating with oocyte quality have been identified, such as the composition and amount of accumulated maternal mRNAs, the size of the oocyte and its zona pellucida, its cytoplasmic organization, or the presence of certain epigenetic modifications (Cran, 1985; Eppig et al, 1994; Fair et al, 1995; Hyttel et al, 1989; La et al, 2019). However, a consensus on the individual power of these parameters to predict oocyte developmental potential has never been reached, and often with conflicting results (Rienzi et al, 2011).…”

“…In the context of assisted reproductive technologies, in vitro oocyte maturation can be used to avoid hormonal treatments for women experiencing repeated in vitro fertilization failures or who do not respond to hormonal stimulation (Hardy et al, 2000;La et al, 2019;Lonergan and Fair, 2016;Hatırnaz et al, 2018). Moreover, oocytes can also be extracted and frozen for fertility preservation.…”