Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development?

Abstract

Currently, rescue in vitro maturation (IVM) is not a routine method in assisted reproductive treatment (ART) programmes but is a promising procedure for ART to improve IVM. The aim of this study was to compare embryo morphokinetics of germinal vesicles (GV) with metaphase II (MII) oocytes from controlled ovarian hyperstimulation (COH) cycles by timelapse photography monitoring (TLM). Morphokinetics of the same number of embryos derived from the in vivo (group I) and rescue of in vitro matured oocytes (group II) from 310 patients were analyzed and compared retrospectively. The time to form second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf) and time of two to eight discrete cells (t2-t8) were assessed. Abnormal cleavage patterns such as uneven blastomeres at the two-cell stage, cell fusion (Fu), trichotomous mitoses (TM), and the rates of embryo arrest were assessed. These data showed that tPB2, tPNa, tPNf, t2, t3 and t4 stages took place later in group II compared with group I (P < 0.001, P = 0.017, P < 0.001, P < 0.001, P < 0.001, P < 0.001, respectively). The rates of uneven blastomeres, Fu, TM, and embryo arrest were increased significantly in group II compared with group I (P = 0.001, P < 0.001, P = 0.003, P < 0.001, respectively). Based on the exact annotation of timing parameters and cleavage patterns, the present data agreed with the concept that rescue IVM of oocytes negatively influences embryo morphokinetics. Therefore, cautious use of embryos derived from rescue IVM of GV oocytes should be made. Zygote cambridge.org/zyg Research Article Cite this article: Faramarzi et al. (2018) Does rescue in vitro maturation of germinal vesicle stage oocytes impair embryo morphokinetics development? Zygote 26: 430-434.

Analysis of maturation dynamics and developmental competence of in vitro matured oocytes under time-lapse monitoring

“…Given that R-IVM is still an experimental technique with controversies [ 41 , 42 ], embryo transfer was not performed and implantation rates and pregnancy outcomes could not be assessed, which is the main limitation of the current study. Comparing with the classical IVM, during which the COCs were mostly used instead of the denuded oocytes, the developmental competence of oocytes in our study was relatively lower [ 26 , 43 ].…”

IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

“…Assessment of the embryos was complete according to the strict time points of every embryological happening and the situation of their time points in the “normal” range, as suggested previously (Armstrong, Bhide, Jordan, Pacey, & Farquhar, ; Basile et al, ; Meseguer et al, ). We checked some morphokinetic variables for embryo selection including (a) t2: time to two cells (24–28 hr), (b) cc2: time between division from two to three cells (8–12 hr), (c) t3: time to three cells (30–38 hr), (d) s2: time among split from three to four cells (<45 min), (e) t4: time to four cells (35–41 hr), (f) t5: time to five cells (48–57 hr), (g) s3: time among split from five to eight cells (<6 hr) and (h) t8: time to eight cells (50–59 hr), as mentioned earlier (Basile et al, ; Faramarzi et al, ; Meseguer et al, ). For the selection of the best embryos, we used the Meseguer's algorithm that happening with a morphological selection of the embryos: those that were visibly not good were rejected and not proposed for embryo transfer (ET).…”

“…Morphokinetic facts also help the embryologists to categorise unclear timing varieties between developing times in embryos with similar form (Yang et al, ). The time‐lapse monitoring system (TLM) has prepared unceasing observing of embryo growth during the culture (Faramarzi, Khalili, Ashourzadeh, & Palmerini, ; Faramarzi, Khalili, Micara, & Agha‐Rahimi, ). Only one study assessed the developing dynamics of IMSI‐derived embryos in teratozoospermia patients using TLM (Knez, Tomazevic, Vrtacnik‐Bokal, & Virant‐Klun, ).…”

Growth differentiation factor 9 and cumulus cell supplementation in in vitro maturation culture media enhances the viability of human blastocysts

“…In controlled ovarian hyperstimulation (COH), 15% of oocytes are immature at the germinal vesicle (GV) or metaphase I (MI) stages. Rescue IVM of these immature oocytes can increase the chances of embryo access, especially in poor responders [1,2]. In addition, the IVM technique plays a highly important role in the elimination of ovarian hyperstimulation syndrome, which is a possible side effect of gonadotropin stimulation in patients with polycystic ovary syndrome (PCOS) [3,4].…”