IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

Mangoli, Esmat; Khalili, Mohammad Ali; Talebi, Ali Reza; Agha‐Rahimi, Azam; Soleimani, Masoud; Faramarzi, Azita; Pourentezari, Majid

doi:10.1111/and.13340

Cited by 22 publications

(23 citation statements)

References 40 publications

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“…Our results were different from other studies (Mangoli et al., 2019, 2020). It was noted that sperm selection through ICSI led to the delay of S1, T4, S2 and T5 in embryos of infertile men diagnosed with oligoasthenoteratozoospermia when compared to other aetiologies.…”

Section: Discussioncontrasting

confidence: 99%

“…To the best of our knowledge, our study is the first to answer the raising question whether the two sperm selection modalities influence embryo morphokinetics as well as reduce the incidence of anomaly development derived from sub‐optimal spermatozoa upon the blastocyst stage, while two studies have investigated until the cleavage stage (day 3) (Mangoli et al., 2019, 2020). Differences between three kinetic time points (T7, TEB and CC3) of IMSI‐ and ICSI‐derived embryos were apparent while other morphokinetic parameters did not differ between the two sperm selection methods.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated that sperm selection through IMSI has significantly improved fertilisation rates, embryo quality, blastocyst rates and clinical pregnancy (Bartoov et al., 2002; Luna et al., 2015; Mangoli et al., 2019). IMSI has also been proposed to improve the embryo quality and clinical pregnancy rate of specific groups, particularly those with previously unsuccessful IVF‐ICSI cycles (Delaroche et al., 2013; Shalom‐Paz et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

Boediono

Handayani²,

Sari³

et al. 2021

Andrologia

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An investigation was conducted to determine the influence of two sperm selection modalities, IMSI and ICSI, on the morphokinetics, dynamic development and ploidy status of embryos derived from males with sub‐optimal sperm profiles during IVF program. A total of 209 PGTA‐tested top‐quality blastocysts (IMSI = 129, ICSI = 80) from 84 couples (IMSI = 51, ICSI = 33) were assessed retrospectively. This study found that both IMSI and ICSI yielded comparable embryo morphokinetics, except for the T7, TEB and CC3 parameters (p < 0.05). A significant lower incidence of multinucleation was observed in the IMSI group when compared to the ICSI group (48.8% vs. 71.3%, p = 0.002), while other parameters of embryo development such as direct cleavage, distorted cytoplasmic movement, reverse cleavage and vacuole(s) appearance did not differ (p > 0.05). No differences were noticed in the proportion of generating chromosomally euploid embryos (44.2% vs. 51.3%, p = 0.394, respectively, for IMSI and ICSI). The implementation of IMSI or ICSI in couples with sub‐optimal sperm profiles resulted in embryos with comparatively similar morphokinetics. Furthermore, the incidence of multinucleation at the two‐ to four‐cell stage was lower following the practice of IMSI, although the method did not improve the proportion of gaining euploid embryos.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

Boediono

Handayani²,

Sari³

et al. 2021

Andrologia

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“…Using the motile sperm organelle morphology examination (MSOME) technique, in a series of 10 patients with teratozoospermia, Knez et al 7 demonstrated that head vacuoles of injected spermatozoa affect morphokinetic parameters and implantation rates. In another study published recently, 8 embryos of OAT patients exhibited protracted developmental dynamics compared to other sperm pathologies—asthenozoospermia, asthenoteratozoospermia, and teratozoospermia.…”

Section: Introductionmentioning

confidence: 90%

Does sperm origin—Ejaculated or testicular—Affect embryo morphokinetic parameters?

et al. 2020

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Background It is unclear whether sperm origin, either ejaculated or testicular, in couples diagnosed with male factor infertility, affects the timing of the embryo's developmental events evaluated by time‐lapse monitoring and implantation rates. Objective To examine the effect of sperm origin on embryo morphokinetics in couples diagnosed with male factor infertility. Materials and Methods This study included a retrospective analysis of morphokinetic parameters performed by time‐lapse monitoring between 2013 and 2017. The developmental processes and morphokinetic parameters of 419 embryos obtained from couples with male factor infertility attributed to oligo‐astheno‐teratozoospermia, 158 embryos derived from surgically extracted testicular spermatozoa from couples diagnosed with non‐obstructive azoospermia, and 190 embryos from couples with normal ejaculated spermatozoa and female mechanical factor‐related infertility, were evaluated. A comparison of morphokinetic parameters, implantation, and clinical pregnancy rates was performed between the groups with additional analysis in accordance with implantation status. Results Embryos from the normal ejaculated spermatozoa and oligo‐astheno‐teratozoospermia patients reached the later morphokinetic milestones—synchronous division (S3) and time to morula (tM)—faster than embryos obtained from testicular spermatozoa. Implantation rate was similar in the normal ejaculated spermatozoa and oligo‐astheno‐teratozoospermia groups (41.9% vs. 45.8%, NS), with higher implantation rate in the oligo‐astheno‐teratozoospermia group compared to the testicular spermatozoa group (45.8% vs. 33.6%, p = 0.02). Comparison of Known Implantation Data (KID) positive (KIDp) and KID negative (KIDn) embryos in each group revealed more rapid development in KIDp embryos in the normal ejaculated spermatozoa and the oligo‐astheno‐teratozoospermia groups, while in the testicular spermatozoa group implanted embryos reached the late morphokinetic milestones (time to 8 cell stage—t8, ECC3, S3, and tM) significantly faster than embryos that failed to implant. In a multivariate logistic regression analysis of the male factor infertility population, (oligo‐astheno‐teratospermia) (OR = 2.54, p = 0.003) and t8 (OR = 0.95, p = 0.027) were predictive of successful implantation. Male factor infertility embryos that reached the t8 milestone within 48–56 h had favorable implantation rates (p < 0.001). Discussion The study results may highlight another pathophysiology by means of which sperm origin affects embryo developmental kinetics. Selecting embryos demonstrating a faster developmental rate at t8 and specifically the 48‐ to 56 h interval following time of pronuclei fading (tPNf) may improve implantation rates in cases of male factor infertility. Conclusion This study showed that ejaculated spermatozoa is associated with faster late cell divisions, more rapid compaction, and higher implantation rates compared to testicular spermatozoa. Additionally, t8 is an important predictor for implantation in the m...

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“…Combination of morphological and morphokinetic parameters can assist in selecting the embryos with high implantation potential. Time‐laps monitoring (TLM) is a predicting tool for evaluating embryos with higher development potential, implantation and even embryo aneuploidy status (Faramarzi, Khalili, & Jahromi, 2019; Mangoli et al., 2019; Omidi, Khalili, Halvaei, Montazeri, & Kalantar, 2020). This technology allows investigation of embryo development in stable incubator condition (Bhide et al., 2017).…”

Section: Introductionmentioning

confidence: 99%

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

et al. 2020

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Evaluation of sperm integrity may predict the in vitro fertilisation (IVF) outcomes. The aim was to evaluate the relationship between the sperm DNA fragmentation (sDNAf) with embryo morphology and morphokinetic using time‐laps monitoring (TLM) and to select the best time points for normalisation in IVF setting. After evaluating the fertilisation and pronuclei (Z) scoring, 328 normally fertilised oocytes were assessed to time of pronuclei fading, time of 2 to 8 discrete cells (t2–t8) and abnormal cleavage patterns, such as multinucleation, direct cleavage, reverse cleavage and fragmentation. Sperm chromatin dispersion (SCD) assay was used for assessment of prepared sperm chromatin status. SCD was categorised into 4 groups of <6.5, 6.5–10.7, 10.7–20.1 and >20.1. The finding showed significant differences in t6 (p = .012), t7 (p = .045), t8 (p = .013) and s1 (p = .001) between 4 SCD groups. When morphokinetic variables were normalised to tPNf, this difference was observed in t2 (p = .003) and t6 (p = .017). Subsequently, the percentage of top quality embryos and Z1 scoring were dependent to the sDNAf rate. In conclusion, tPNf was the best reference time point in IVF cycles. Also, we found high sDNAf rate had no negative impact on embryo morphology and morphokinetics in conventional IVF.

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IMSI procedure improves clinical outcomes and embryo morphokinetics in patients with different aetiologies of male infertility

Cited by 22 publications

References 40 publications

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

Morphokinetics of embryos after IMSI versus ICSI in couples with sub‐optimal sperm quality: A time‐lapse study

Does sperm origin—Ejaculated or testicular—Affect embryo morphokinetic parameters?

Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

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