Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

Anbari, Fatemeh; Khalili, Mohammad Ali; Agha‐Rahimi, Azam; Maleki, Behnam; Nabi, Ali; Esfandiari, Navid

doi:10.1111/and.13798

Cited by 12 publications

(10 citation statements)

References 39 publications

(41 reference statements)

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“…There is a positive correlation between the incubation time and sperm DNA damage. Prolonged incubation of prepared sperm samples at 37°C was associated with high rates of sperm DNA fragmentation, indicating that the sperm samples intended for ART procedures should be used within 2 hours of incubation at 37°C [33,34] . Interestingly, it has been shown that following 2 and 4 hours of incubation at RT, the sperm progressive motility and viability decreased significantly [35] .…”

Section: Discussionmentioning

confidence: 99%

Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures

Yang

Liu

Xue

et al. 2022

Reproductive and Developmental Medicine

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Objective: The purpose of this study was to evaluate the sperm motility and DNA integrity at different temperatures to analyze whether the sperms are suitable on the second day for insemination of in vitro matured oocytes by intra-cytoplasmic sperm injection (ICSI) following density gradient centrifugation (DGC) and swim-up (SU) procedures. Methods: Semen samples were collected from 30 outpatients who visited the Center for Reproductive Medicine for semen analyses. Following sperm selection by DGC and SU procedures, the liquified semen samples were divided into three groups and incubated at 4, 25, and 37°C, respectively. Following incubation for 24, 48, and 72 hours, the sperm motility and sperm DNA fragmentation index (DFI) were analyzed. Results: Following the combination of DGC and SU procedures, the sperm motility (91.8% ± 8.6% vs. 50.8% ± 13.1%) and DFI (5.1% ± 7.9% vs. 13.0% ± 11.6%) were significantly improved (P <0.01) compared to those without any treatment. The sperm motility of the 3 groups significantly declined (P <0.05) post-incubation compared to that of the groups prior incubation. However, sperm motility significantly increased (76.9% ± 10.4%) (P <0.05) at 25°C compared to that of the other 2 groups (53.5% ± 11.0% and 47.6% ± 10.2%). Sperm DFI significantly increased (P <0.05) at 37°C following incubation for 24 and 72 hours in comparison to that of the other 2 groups. However, the sperm DFI did not significantly increase when the sperm samples were incubated at 4 (5.7% ± 5.9%) and 25°C (6.8% ± 5.6%) for 24 hours compared to that before incubation (5.1% ± 7.9%). Conclusions: These results indicate that the sperm quality, in terms of motility and DFI, can be efficiently improved by DGC in combination with SU. Following which, the sperm samples can be incubated at 25°C and be used on the second day for insemination of in vitro matured oocytes by ICSI.

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Section: Discussionmentioning

confidence: 99%

Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures

Yang

Liu

Xue

et al. 2022

Reproductive and Developmental Medicine

View full text Add to dashboard Cite

show abstract

“…Finally, after a brief wash and dehydration in increasing ethanol baths, the sperm chromatin was stained for visualization under bright-field microscopy. The spermatozoa were scored under the light microscope (×1000) and big or medium halos were considered as spermatozoa without DNA fragmentation while fragmented spermatozoa showed small or no halos ( 18 ).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Efficiency of Magnetic-Activated Cell Sorting (MACS) and Physiological Intracytoplasmic Sperm Injection (PICSI) for Sperm Selection in Cases with Unexplained Infertility

Ahmadi¹,

Sobhani²,

Khalili³

et al. 2022

JRI

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Background: The cases with unexplained infertility may have an abnormality in their sperm chromatin structure. Sperm selection methods can be used to separate sperm with low DNA fragmentation. The purpose of this study was to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) with magnetic-activated cell sorting (MACS) in assisted reproductive techniques in cases with unexplained infertility. Methods: The semen samples were collected from couples with unexplained infertility. After semen analysis and sperm DNA fragmentation (SDF) evaluations, samples were prepared with swim-up method. The rates of SDF in different fractions including raw semen (n=20), swim-up (n=20), only motile sperm after swim-up (swim-up selection) (n=20), MACS sperm selection (n=20), only motile sperm after MACS (MACS selection) (n=20), and PICSI sperm selection (n=16) were evaluated. Also, the main sperm characteristics and fine morphology of sperm suspension after MACS were assessed. Statistical analysis was performed using GraphPad Prism. The p<0.05 was considered statistically significant. Results: DNA fragmentation index (DFI) values in PICSI and MACS groups were significantly reduced as compared to the swim-up group. The rate of this reduction was more pronounced in MACS (58.20±13.02) than PICSI (36.57±15.52) group. Also, our results showed that MACS resulted in decreased sperm motility, with no alteration in their fine morphology. Conclusion: MACS was found to be more efficient in reduction of SDF rates than PICSI. However, none of the sperm selection techniques can not totally eliminated the spermatozoa with DNA fragmentation in the final sperm sample.

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“…Numerous studies emphasize the direct relationship between SDF and male infertility and increased miscarriage rate after IVF or ICSI [ 50 ]. Whereas low SDF can be repaired by the competent oocyte, high levels of SDF were correlated with embryo morphokinetic delay observable from the time of of pronuclei fading to the morula stage [ 51 , 52 , 53 , 54 ]. The consequences are lower blastulation and pregnancy rates [ 55 ].…”

Section: Spermatozoon Effect On Embryo Developmentmentioning

confidence: 99%

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

Chamayou

Giacone

Cannarella

et al. 2023

JCM

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The intracytoplasmic sperm injection (ICSI) technique was invented to solve severe male infertility due to altered sperm parameters. Nowadays, it is applied worldwide for the treatment of couple infertility. ICSI is performed with any available spermatozoon from surgery or ejaculated samples, whatever are the sperm motility, morphology or quantity. The aim of the present review was to study if embryo development and kinetics would be modified by (1) ICSI under the technical aspects, (2) the micro-injected spermatozoa in connection with male infertility. From published data, it can be seen that ICSI anticipates the zygote kinetics Furthermore, because fertilization rate is higher in ICSI compared to conventional in vitro fertilization (IVF), more blastocysts are obtained for clinical use in ICSI. Sperm and spermatozoa characteristics, such as sperm parameters, morphology and vitality, DNA content (levels of sperm DNA fragmentation, microdeletions, and chromosomal abnormalities), RNA content, epigenetics, and sperm recovery site (testicular, epididymis, and ejaculated), have an impact on fertilization and blastocyst rates and embryo kinetics in different ways. Even though ICSI is the most common solution to solve couples’ infertility, the causes of male infertility are crucial in building a competent spermatozoa that will contribute to normal embryonic development and healthy offspring.

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Does sperm DNA fragmentation have negative impact on embryo morphology and morphokinetics in IVF programme?

Cited by 12 publications

References 39 publications

Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures

Changes in human sperm motility and DNA fragmentation index after incubation at different temperatures following density gradient centrifugation and swim-up procedures

Comparison of the Efficiency of Magnetic-Activated Cell Sorting (MACS) and Physiological Intracytoplasmic Sperm Injection (PICSI) for Sperm Selection in Cases with Unexplained Infertility

What Does Intracytoplasmic Sperm Injection Change in Embryonic Development? The Spermatozoon Contribution

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