Molecular linkage map of allotetraploid cotton (Gossypium hirsutum L. × Gossypium barbadense L.) with a haploid population

Abstract

In the present study, a haploid population from the cross of the two cultivated allotetraploid cottons, Gossypium hirsutum L. and Gossypium barbadense L., was developed by means of Vsg, a virescently marked semigamous line of Sea island cotton, and some target haploids were successfully doubled with colchicine. A molecular linkage map was constructed with 58 doubled and haploid plants. Among the total of 624 marker loci (510 SSRs and 114 RAPDs), 489 loci were assembled into 43 linkage groups and covered 3,314.5 centi-Morgans (cM). Using the monosomic and telodisomic genetic stocks, the linkage groups of the present map were associated with chromosomes of the allotetraploid genome, and some of the unassociated groups were connected to corresponding A or D subgenomes. Through the analysis of the assignment of the duplicated SSR loci in the chromosomes or the linkage groups, ten pairs of possible homoeologous chromosome (or linkage group) regions were identified. Among them, the pairs of Chrs. 1 and 15, Chrs. 4 and 22, and Chrs. 10 and 20 had already been determined as homoeologous by classical genetic and cytogenetic research, and the pair of Chrs. 9 and 23 had also been identified by the ISH method of molecular cytogenetics. But, from present research, it was assumed that Chrs. 5 and 18 might be a new pair of homoeologous chromosomes of the allotetraploid cotton genome detected by molecular mapping of the cotton genome.

Inheritance of long staple fiber quality traits of Gossypium barbadense in G. hirsutum background using CSILs

“…SSR-PCR amplifications were performed using a Peltier Thermal Cycler-225 (MJ Research) and electrophoresis of PCR products was performed as described by Zhang et al (2000Zhang et al ( , 2002.…”

Genetic improvement of Gossypium arboreum L. using molecular markers: Status and development needs

“…The poor reproducibility of RAPD profiles within and between laboratories can be attributed to various factors like quality of DNA, composition of PCR reaction mixture, nature of DNA polymerase and the skills of the working person, to quote a few. RAPDs have been used for diversity, genome mapping and phylogenetic studies in cotton (Rahman et al, 2002b;Zhang et al, 2002;He et al, 2007;Rahman et al, 2008b;Rana and Bhat 2004). RAPDs are not popular for genetic mapping and gene tagging studies in cotton because of their low informativeness and non-locus specific nature.…”

“…SNP analysis is useful for cultivar discrimination in crops where it is difficult to find polymorphism. These DNA markers have also been used in cotton for fingerprinting (Rahman et al, 2002b(Rahman et al, , 2008b, linkage map construction (Reinisch et al, 1994;Zhang et al, 2002;Lacape et al, 2003;Mei et al, 2004;Rong et al, 2004), gene mapping (Shappley et al, 1998;Ulloa and Meredith, 2000) and genetic diversity studies (Rahman et al, 2002b(Rahman et al, , 2008b.…”

Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L.

“…As the segregation of phenotype (male fertility/sterility) in F 2 s did not fit a one gene ratio of 3:1 perfectly, the two SSR primers S1231 and S1317, which could produce polymorphic DNA between parents and were not Heterogeneity test 0.0001 >0.995 mapped in the same linkage group (Zhang et al 2002) as those tightly linked with Rf 1 presented later, were used to test whether the molecular markers are segregating normally in the F 2 in which Rf 1 did not fit a 3:1 segregation. The primer S1231 generated a co-dominant maker S1231 210 and a dominant maker S1231 140 .…”