Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L.

Liu, L.; Guo, Wenshan; Zhu, Xiefei; Zhang, T.

doi:10.1007/s00122-002-1084-0

Cited by 83 publications

(50 citation statements)

References 26 publications

(31 reference statements)

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“…Genomic DNA from five wheat cultivars and from Chinese Spring nulli-tetrasomic lines were extracted from young leaves using a modified CTAB protocol (Liu et al, 2003). Amplification by PCR was performed using a T-100 ThermalCycler (BIO-RAD) and reactions consisting of 2.0 mM MgCl 2 , 0.2 mM dNTPs, 0.75 U Taq DNA polymerase (TaKaRa Bio Inc., Dalian, China), 0.1 µM of each primer, and 10 ng template DNA in a final reaction volume of 15 µL.…”

Section: Dna Extraction Pcr Amplification and Detectionmentioning

confidence: 99%

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

2016

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ABSTRACT. Simple sequence repeats (SSRs) are highly informative, polymorphic, and co-dominant Mendelian markers that provide an important genomic resource for genetic research. Recently, the use of large-scale transcriptome sequence has become a reliable and efficient approach for the identification and development of new EST-SSR markers. In this study, 8389 potential SSRs with a minimum of five repetitions for all motifs were identified from 121,210 unigenes. Gene ontology analysis indicated that the unigenes containing SSR loci participate in various biological processes of regulation, growth, development, metabolism, and apoptosis in wheat. As in many other plants, trinucleotide repeats were found to be the most abundant repeat units with a frequency of 62.33%. A subset of 300 EST-SSRs was randomly selected for the applicability of EST-SSRs to be evaluated. Of the 300 primer pairs tested, 177 (59%) yielded unambiguous PCR products among five wheat cultivars. Using the Chinese Spring nullitetrasomic line, 131 of the 177 EST-SSR primer pairs yielded products and 178 loci were found to be located on all the 21 wheat chromosomes. These findings suggest that the novel EST-SSR markers, as a basis for future genetic linkage and gene tagging analysis, are a valuable tool for genetic mapping, marker assisted selection, and comparative genome analysis.

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Section: Dna Extraction Pcr Amplification and Detectionmentioning

confidence: 99%

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

2016

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“…Genomic DNA was isolated from radish root using a modified CTAB method (Liu et al, 2003). Total RNA was isolated from radish samples using a Simple P total RNA extraction kit (Tiangen Biotech Co., Ltd., Beijing, China), following the manufacturer instructions.…”

Section: Isolation Of Cdna and Genomic Dna Sequencesmentioning

confidence: 99%

Characterization of RsMYB28 and RsMYB29 transcription factor genes in radish (Raphanus sativus L.)

Luo

Liu

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Glucosinolates (GSLs) are important secondary metabolites in Brassicaceae plants. Previous studies have mainly focused on GSL contents, types, and biosynthesis-related genes, but the molecular characterization patterns of GSL biosynthesis-related transcription factors remain largely unexplored in radish (Raphanus sativus L.). To isolate transcription factor genes regulating the GSL biosynthesis, genomic DNA and cDNA sequences of RsMYB28 and RsMYB29 genes were isolated in radish. Two R2R3-MYB domains were identified in the deduced amino acid sequences. Subcellular localization and yeast-one hybrid assays indicated that both the RsMYB28 and RsMYB29 genes were located in the nucleus and possessed transactivation activity. Reverse transcription quantitative analysis showed that the RsMYB28 and RsMYB29 genes were expressed in seeds, leaves, stems, and roots at the seedling, taproot thickening, and mature stages. Both genes were highly expressed during the seedling and taproot thickening stages. The expression level of RsMYB28 was found to be up-regulated following wounding, glucose, and abscisic acid treatments, whereas RsMYB29 was up-regulated following wounding and methyl jasmonate treatments. These results provide insights into the biological function and characterization of the RsMYB28 and RsMYB29 genes, and facilitate further dissection of the molecular regulatory mechanism underlying the GSL biosynthesis in radish.

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“…Among these samples, Ajania pacifica is native to the Taiwanese area of China and Japan. Genomic DNA was isolated from young leaves using an improved cetyltrimethylammonium bromide method (Liu et al, 2003 …”

Section: Plant Materials and Dna Extractionmentioning

confidence: 99%

Development of expressed sequence tag-simple sequence repeat markers for Chrysanthemum morifolium and closely related species

Sun

et al. 2015

Genet. Mol. Res.

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ABSTRACT. With the development of chrysanthemum breeding in recent years, an increasing number of wild species in genera related to Chrysanthemum were introduced to extend the genetic resources and facilitate the genetic improvement of chrysanthemums via hybridization. However, few simple sequence repeat (SSR) markers are available for marker-assisted breeding and population genetic studies of chrysanthemum and closely related species. Expressed sequence tags (ESTs) in public databases and cross-species transferable markers are considered to be a cost-effective means for developing sequencebased markers. In this study, 25 EST-SSRs were successfully developed from Chrysanthemum EST sequences for Chrysanthemum morifolium and closely related species. In total, 4164 unigene sequences were assembled from 7180 ESTs of chrysanthemum in GenBank, which were subsequently used to screen for the presence of microsatellites with the Development of EST-SSR markers for chrysanthemum SSRIT software. The screening criteria were 8, 5, 4, and 3 repeating units for di-, tri-, tetra-, and penta-and higher-order nucleotides, respectively. Moreover, 310 SSR loci from 296 sequences were identified, and 198 primer pairs for SSR amplification were designed with the Primer Premier 5.0 software, of which 25 SSR loci showed polymorphic amplification in 52 species and varieties belonging to Chrysanthemum, Ajania, and Opisthopappus. The application of EST-SSR markers to the identification of intergeneric hybrids between Chrysanthemum and Ajania was demonstrated. Therefore, EST-SSRs can be developed for species that lack gene sequences or ESTs by utilizing ESTs of closely related species.

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Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L.

Cited by 83 publications

References 26 publications

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

Identification of novel and useful EST-SSR markers from de novo transcriptome sequence of wheat (Triticum aestivum L.)

Characterization of RsMYB28 and RsMYB29 transcription factor genes in radish (Raphanus sativus L.)

Development of expressed sequence tag-simple sequence repeat markers for Chrysanthemum morifolium and closely related species

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