Inheritance of long staple fiber quality traits of Gossypium barbadense in G. hirsutum background using CSILs

Abstract

Gossypium hirsutum is a high yield cotton species that exhibits only moderate performance in fiber qualities. A promising but challenging approach to improving its phenotypes is interspecific introgression, the transfer of valuable traits or genes from the germplasm of another species such as G. barbadense, an important cultivated extra long staple cotton species. One set of chromosome segment introgression lines (CSILs) was developed, where TM-1, the genetic standard in G. hirsutum, was used as the recipient parent and the long staple cotton G. barbadense Hai7124 was used as the donor parent by molecular marker-assisted selection (MAS) in BC(5)S(1–4) and BC(4)S(1–3) generations. After four rounds of MAS, the CSIL population was comprised of 174 lines containing 298 introgressed segments, of which 86 (49.4%) lines had single introgressed segments. The total introgressed segment length covered 2,948.7 cM with an average length of 16.7 cM and represented 83.3% of tetraploid cotton genome. The CSILs were highly varied in major fiber qualities. By integrated analysis of data collected in four environments, a total of 43 additive quantitative trait loci (QTL) and six epistatic QTL associated with fiber qualities were detected by QTL IciMapping 3.0 and multi-QTL joint analysis. Six stable QTL were detected in various environments. The CSILs developed and the analyses presented here will enhance the understanding of the genetics of fiber qualities in long staple G. barbadense and facilitate further molecular breeding to improve fiber quality in Upland cotton.

Insights into Interspecific Hybridization Events in Allotetraploid Cotton Formation from Characterization of a Gene-Regulating Leaf Shape

“…Hai7124 with a sea-island leaf and the recurrent parent G. hirsutum accession TM-1 ( Figure 1A). Although it is phenotypically a sea-island leaf plant (Wang et al 2012), the CSIL line is a near-isogenic line to TM-1. IL-15-5-1 was crossed as a male parent with TM-1 to produce a mapping population.…”

“…Eighty-nine accessions from seven races of G. hirsutum were listed in Table S2, provided by the Institute of Cotton Research of the Chinese Academy of Agricultural Sciences (CAAS). IL-15-5-1 was derived from a CSIL with sea-island leaves that carried the Hai7124 segment from chromosome D1 in the background of the recurrent parent TM-1 (Wang et al 2012). Two F 2 mapping populations of 770 and 508 individuals were developed from a cross between TM-1 as the female parent and IL-15-5-1 as the male parent.…”

Genetic Mapping in Cotton

“…Mapping populations are obtained using two contrasting parents for the desired trait. In self-pollinated crops; usually mapping populations include F 2 [56,57]; F 2:3 [58,59], recombinant inbred lines (RILs) [60,61], backcross (BC) [62,63], Backcross inbred lines (BILs) [64], near isogenic lines [65,66], doublehaploids [67,68], chromosome substitution lines (CSILs) [69,70]. F 2 , BC, RILs and doublehaploids have been highly used for linkage mapping studies in cotton.…”

“…It is manifested by high heritability-ranged from 0.52 to 0.90 for fiber bundle strength and 0.46 to 0.79 for staple length [146][147][148][149]. Previous studies have shown that staple length is highly under the influence of the genotype [69]. In the present cultivated varieties, significantly low variations for the staple length were observed-thus hampering the future breeding progress because of low genetic diversity available for the trait [25,150,151].…”

Comparison of wild accessions of g. barbadense l. from Peru and Brazil via microsatellite markers

“…is cytogenetically subdivided, and based on meiotic compatibility, it is divided in eight genomic groups (A-G and K). Of the total species, only four were domesticated by humans to produce fiber: G. herbaceum L. (2n = 2x = 26), G. arboreum L. (2n = 2x = 26), G. hirsutum L. (2n = 4x = 52), and G. barbadense L. (2n = 4x = 52) (WANG et al, 2012).…”