Molecular identification and functional characterization of cytochrome P450 monooxygenases from the brown-rot basidiomycete Postia placenta

Abstract

We explored the molecular diversity and functional capabilities of cytochrome P450 monooxygenases (P450s) from the brown-rot basidiomycete Postia placenta. Using bioinformatic and experimental data, we found 250 genes of P450s in the whole genome, including 60 putative allelic variants. Phylogenetic analysis revealed the presence of 42 families, including 18 novel families. Comparative phylogenetic analysis of P450s from P. placenta and the white-rot basidiomycete Phanerochaete chrysosporium suggested that vigorous gene duplication and molecular evolution occurred after speciation of basidiomycetes. Among the 250 gene models, 184 were isolated as full-length cDNA and transformed into Saccharomyces cerevisiae to construct a functional library in which recombinant P450s were co-expressed with yeast NADPH-P450 oxidoreductase. Using this library, the catalytic potentials of P450s against a wide variety of compounds were investigated. A functionomic survey allowed the discovery of novel catalytic properties of P. placenta P450s. The phylogenetic diversity of the CYP53 family in P. placenta was clear, and CYP53D2 is capable of converting stilbene derivatives. This is the first report of this peculiar function of the CYP53 family. Our increased understanding of the molecular and functional diversity of P450s in this fungus will facilitate comprehension of metabolic diversity in basidiomycetes and has future biotechnology applications.

Localizing gene regulation reveals a staggered wood decay mechanism for the brown rot fungus Postia placenta

“…2 and Dataset S3). Some cytochrome P450s are likely involved in aromatic hydroxylation reactions required for methoxyhydroquinone biosynthesis, as well as the hydroxylation of lignin fragments preparatory to aromatic ring fission (28).…”

Molecular and Functional Diversity of Fungal Cytochrome P450s

“…In addition, basidiomycetous P450s have been reported from comparative phylogenetic analyses of P450s in the white-rot basidiomycete Phanerochaete chrysosporium and the brownrot basidiomycete Postia placenta. 16) CYP5027, CYP5350, and CYP5348 are large families in P. placenta but are absent from P. chrysosporium (Table 1). In contrast, the CYP5144 family has been enlarged in P. chrysosporium but not in P. placenta.…”

“…To better understand the metabolic diversity of fungi, it is of great importance to exploit the catalytic functions of numerous P450s. [14][15][16][17][18] In addition to the crucial biological roles of P450s, utilization of their catalytic functions is of great interest in the biotechnology sector. [19][20][21][22] Biocatalytic regio-and stereo-specific oxidations by P450s attract much attention in industry because (i) use of biocatalysts allows reduction in the number of tedious steps such as blocking and deblocking that are common in conventional chemical synthesis, (ii) a single product without by-product formation reduces the cost of downstream purification steps, and (iii) enantiomeric mixtures (rather than racemic mixtures) are strictly required in some fields such as the pharmaceutical industry.…”

Transcriptome Sequencing and Comparative Analysis of Piptoporus betulinus in Response to Birch Sawdust Induction

“…The findings of Krueger et al [38] indicated that brown-rot fungus Gloeophyllum trabeum might be suitable for the biodegradation of styrene. Although white-rot fungi degrading xenobiotic compounds was originally attributed to its lignin-degrading enzyme system, Ide et al [39] suggested that cytochrome P450 showed a novel catalytic property in xenobiotic metabolism. All annotated enzymes or degradation processes could be found in P. betulinus transcriptome, demonstrating that P. betulinus could not only harbor metabolic xenobiotic materials but also potentially degradation of environmental pollutants.…”