Molecular and Functional Diversity of Fungal Cytochrome P450s

Ichinose, Hirofumi

doi:10.1248/bpb.35.833

Cited by 27 publications

(18 citation statements)

References 35 publications

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“…Cytochrome P450s (CYP) play essential roles in the fungal biosynthesis of secondary metabolites and detoxification of toxic compounds [60]–[64]. Both Foc genomes encode a great number of putative CYP genes (173 CYPs in Foc1 and 176 in Foc4, Table 3), some of which are similar to the characterized fungal CYP sequences including the monooxygenase gene BcBOT1 (PHI: 438) and the demethylases genes PDAT9 and PDA6-1 , the products of which are either involved in phytotoxin biosynthesis in Botrytis cinerea (BcBOT1) [65], or participate in detoxify the phytoalexin pisatin from garden pea (PDAT9 and PDA6-1) [63], [66].…”

Section: Resultsmentioning

confidence: 99%

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

et al. 2014

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BackgroundThe asexual fungus Fusarium oxysporum f. sp. cubense (Foc) causing vascular wilt disease is one of the most devastating pathogens of banana (Musa spp.). To understand the molecular underpinning of pathogenicity in Foc, the genomes and transcriptomes of two Foc isolates were sequenced.Methodology/Principal FindingsGenome analysis revealed that the genome structures of race 1 and race 4 isolates were highly syntenic with those of F. oxysporum f. sp. lycopersici strain Fol4287. A large number of putative virulence associated genes were identified in both Foc genomes, including genes putatively involved in root attachment, cell degradation, detoxification of toxin, transport, secondary metabolites biosynthesis and signal transductions. Importantly, relative to the Foc race 1 isolate (Foc1), the Foc race 4 isolate (Foc4) has evolved with some expanded gene families of transporters and transcription factors for transport of toxins and nutrients that may facilitate its ability to adapt to host environments and contribute to pathogenicity to banana. Transcriptome analysis disclosed a significant difference in transcriptional responses between Foc1 and Foc4 at 48 h post inoculation to the banana ‘Brazil’ in comparison with the vegetative growth stage. Of particular note, more virulence-associated genes were up regulated in Foc4 than in Foc1. Several signaling pathways like the mitogen-activated protein kinase Fmk1 mediated invasion growth pathway, the FGA1-mediated G protein signaling pathway and a pathogenicity associated two-component system were activated in Foc4 rather than in Foc1. Together, these differences in gene content and transcription response between Foc1 and Foc4 might account for variation in their virulence during infection of the banana variety ‘Brazil’.Conclusions/SignificanceFoc genome sequences will facilitate us to identify pathogenicity mechanism involved in the banana vascular wilt disease development. These will thus advance us develop effective methods for managing the banana vascular wilt disease, including improvement of disease resistance in banana.

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Section: Resultsmentioning

confidence: 99%

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

et al. 2014

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“…). Cytochrome P450s comprise a large superfamily of heme‐containing monooxygenases with the greatest diversity of all kingdoms observed among fungi: currently, 2 487 fungal cytochrome P450 species have been identified and assigned to 399 families (see: Ichinose for a review) with an apparently greater diversity among brown‐rot fungi relative to white‐rot fungi (Ide et al. ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome of an Armillaria root disease pathogen reveals candidate genes involved in host substrate utilization at the host–pathogen interface

et al. 2013

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Summary Armillaria species display diverse ecological roles ranging from beneficial saprobe to virulent pathogen. Armillaria solidipes (formerly A. ostoyae), a causal agent of Armillaria root disease, is a virulent primary pathogen with a broad host range of woody plants across the Northern Hemisphere. This white‐rot pathogen grows between trees as rhizomorphs and attacks sapwood as mycelial fans under the bark. Armillaria root disease is responsible for reduced forest productivity due to direct tree mortality and non‐lethal infections that impact growth. Here, we characterize a transcriptome of a widespread, virulent genet (vegetative clone) of A. solidipes isolated from a mycelial fan on a natural grand fir (Abies grandis) sapling in northern Idaho, USA. cDNA from polyA+‐purified total RNA was sequenced using a single‐end read approach on the Illumina GAIIx platform which generated 24 170 384 reads. A BLASTx search against the NCBI nr database using 39 943 de novo assembled contigs resulted in 24 442 sequences with significant hits (e‐value < 1e−3), predominantly to fungi (85%). A filtered data set of 20 882 assembled transcripts that encoded putative homologous fungal proteins was created and used for all subsequent analyses. Signal P identified 10 668 putative signal peptides from these fungal transcripts, and 14 360 were annotated with gene ontology terms. Several sequences showed strong homology to annotated genes with functions in pathogenesis, specifically those involved in plant cell wall degradation and response to the post‐infection host environment. This transcriptome contributes to the growing body of resources for studies on fungal pathogens of woody plants, and our results provide useful insights towards identifying specific genes with potential roles associated with pathogenesis and other metabolic functions.

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“…Many fungi are capable to degrade environmental pollutants, break down organic materials and produce secondary metabolites to adapt to various environmental conditions. This results in P450s with an enormous diversity to be found in fungal genomes . Especially multiple phytopathogenic and plant degrading fungi have multiple P450s .…”

Section: Resultsmentioning

confidence: 99%

Accessing the Biocatalytic Potential for C−H‐Activation by Targeted Genome Mining and Screening

et al. 2019

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Cytochrome P450 monooxygenases (P450s) are ubiquitous hemeproteins that insert oxygen specifically into substrates leading to diverse chemical transformations. Utilizing their capabilities, microbial whole-cell biocatalysts are applied in pharmaceutical and fine chemical industry to produce biomolecules and drug metabolites. In order to synthesize novel bioactive compounds there is a great demand to identify P450s with new reaction and substrate scope. In this study, genome mining and an activity screening were successfully combined to discover so far underutilized biocatalysts. The screening revealed the expected broad range of reactions, such as hydroxylations, dealkylations, reductions and desaturations. For Actinosynnema mirum and ritonavir the biotransformation was transferred to a preparative scale resulting in a ritonavir conversion of 90 % after 48 h and 13 different metabolites analyzed by LC-MS 2 and NMR. These results clearly demonstrate the potential of the underlying approach to identify promising whole cell biocatalysts with good conversion and product scopes.[a] L. . Bacterial and fungal strains selected for activity screening. Shown are total numbers of found P450 sequences for CYPED (black) and PROSITE (green) and the number of homologous families (brown) and superfamilies (red). A: bacterial strains 1 Bradyrhizobium elkanii, 2 Cystobacter fuscus DSM 2262, 3

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Molecular and Functional Diversity of Fungal Cytochrome P450s

Cited by 27 publications

References 35 publications

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

Genome and Transcriptome Analysis of the Fungal Pathogen Fusarium oxysporum f. sp. cubense Causing Banana Vascular Wilt Disease

Transcriptome of an Armillaria root disease pathogen reveals candidate genes involved in host substrate utilization at the host–pathogen interface

Accessing the Biocatalytic Potential for C−H‐Activation by Targeted Genome Mining and Screening

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