Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron

Kumar, Anil; Sood, Anil K.; Palni, Uma T.; Gupta, Akshey K.; Palni, L. M. S.

doi:10.1080/14620316.2001.11511322

Cited by 30 publications

(17 citation statements)

References 22 publications

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“…BA at concentrations of 5-20 lM yielded the highest number of shoots and was most suitable for the in vitro rapid multiplication of ''FM1''. This is in agreement with the results of the reports in other rose species (Khosh-Khui and Sink 1982;Skirvin et al 1990;Compas and Pais 1990;Kumar et al 2001;Jabbarzadeh and Khosh-Khui 2005). BA induced chimeral segregation in vitro.…”

Section: Discussionsupporting

confidence: 95%

In vitro separation of a rose chimera

Canli

Skirvin

2008

Plant Cell Tiss Organ Cult

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Section: Discussionsupporting

confidence: 95%

In vitro separation of a rose chimera

Canli

Skirvin

2008

Plant Cell Tiss Organ Cult

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“…Slight induction of callus from the base of the cultures was observed on medium with 1.2 μM KN, which increased in the presence of 2.3 μM KN. These results agree with reports in which a two-stage culture consisting of TDZ in the first medium and another plant growth regulator in the second medium promote shoot development in other species (Eapen et al 1998;Kumar et al 2001;Thomas 2003). Shoot bud induction as well as elongation was achieved in the presence of TDZ in peanut (Matand and Prakash 2007).…”

Section: Resultssupporting

confidence: 94%

In vitro germination and micropropagation of Givotia rottleriformis Griff.

Samuel

Debashish

Madhumita

et al. 2009

In Vitro Cell.Dev.Biol.-Plant

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The propagation of Givotia rottleriformis Griff. is difficult as a result of long seed dormancy associated with poor seed germination. The present study was undertaken to develop a protocol to overcome seed dormancy by culture of zygotic embryo axes and then develop an efficient method for micropropagation of Givotia. Best germination frequency (78.3%) was achieved from mature zygotic embryo axes isolated from acidscarified fresh seeds when cultured on Murashige and Skoog (MS) medium (half-strength major salts) with 28.9 μM gibberellic acid (GA 3 ). Efficient plant conversion was achieved by transfer of 10-d-old germinated embryos to MS medium (half-strength major salts) supplemented with 1.2 μM kinetin (KN) and 0.5 μM indole-3-butyric acid (IBA). However, acid scarification of 1-yr-old seeds decreased the germination frequency of zygotic embryo axes in comparison to those obtained from non-acidscarified seeds which germinated (96.2%) and converted into plants (80.3%) on MS basal (half-strength major salts) medium. Multiple shoot bud induction was achieved by culture of shoot tips derived from in vitro germinated seedlings on MS medium with 0.5 μM thidiazuron for 4 wk, and the shoots elongated after transfer to a secondary medium with 1.2 μM KN. A maximum number of 7.8 shoots per explant with an average shoot length of 3.2 cm was achieved after two subcultures on this medium. The in vitro regenerated shoots rooted (41.5%) on half-strength MS medium with 0.5 μM IBA. The in vitro generated seedlings and micropropagated plants were established in soil with a survival frequency of 70% and 60%, respectively.

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“…However, the presence of TDZ is favorable at lower concentrations and after 1.0 mg/L it caused hyperhydricity. Although cytokinins favored shoot multiplication (Kumar et al, 2001), higher concentrations may cause dramatic effects as happened in this case. According to a previous report (Huetteman and Preece, 1993), TDZ promoted shoot initiation but inhibited shoot elongation at higher concentrations.…”

Section: Direct Organogenesis Studiesmentioning

confidence: 70%

Accumulation of phenolics in natural and micropropagated plantlets of Thymus pseudopulegioides Klokov & Des.-Shost. with their antioxidant potentials

Günaydın¹,

Laghari²,

Bektaş³

et al. 2017

Turk J Biol

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The in vitro production of rosmarinic acid (RA), an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid, has particular importance as it has always been included in important phenolics lists owing to its antioxidant, antiinflammatory, anticancer, and antidiabetic properties, make it attractive in the food industry as well as complementary medicine (Petersen and Simmons, 2003).The organic synthesis of RA is tedious, time-consuming, and costly (Chapado et al., 2010;Brahmachari, 2013). Therefore, producers still rely on natural sources in terms of RA supply and recently in vitro grown plantlets have become more promising (Su et al., 1995;Chapado et al., 2010). The first successful in vitro production of rosmarinic acid was achieved by using cell cultures of Coleus blumei Benth. (Razzaque and Ellis, 1977;Zenk et al., 1977). They successfully produced RA in vitro at 21% (w/w) as dry weight base. Afterwards, many researchers contributed to the accumulation of information concerning RA production in vitro (e.g., Ulbrich et. al., 1985;Pavlov et al.,

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Micropropagation of Rosa damascena Mill. from mature bushes using thidiazuron

Cited by 30 publications

References 22 publications

In vitro separation of a rose chimera

In vitro separation of a rose chimera

In vitro germination and micropropagation of Givotia rottleriformis Griff.

Accumulation of phenolics in natural and micropropagated plantlets of Thymus pseudopulegioides Klokov & Des.-Shost. with their antioxidant potentials

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