Micropropagation and restricted-growth storage of adult oak genotypes

Gebhardt, K.; Frühwacht-Wilms, U; Weisgerber, H.

doi:10.1051/forest:19930734

Cited by 5 publications

(1 citation statement)

References 6 publications

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“…그러나 대조구(25°C)의 배발생조직은 계대배양을 하 지 않을 경우 6개월 후 식물체 재생 능력을 완전히 상실 했다고 하였다. Gebhardt et al (1993) (Moon et al 2008). 최근에 는 여러 종류의 Saponin 및 항산화 물질에 관한 연구 (Shao et al 1990;Porzel et al 1992), 항류머티즘 (Choi et al 2002a, b), 항당뇨병 (Park et al 1998), 항염증성 Lee et al 2001) 등의 약리적인 효과가 보고되어 산업적인 측 면에서의 이용 가능성을 크게 하고 있다.…”

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Somatic embryo induction and plant regeneration from cold-stored embryogenic callus ofK. septemlobus

2015

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Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at 4°C in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at 4°C without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

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