Somatic embryo induction and plant regeneration from cold-stored embryogenic callus ofK. septemlobus

Abstract

Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at 4°C in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at 4°C without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.

Biological activities ofBrassica rapa(Turnip) callus extracts by plant cell culture technology

“…최근에는 식물조직배양기술을 이용하여 생리활성이 우 수한 기능성 천연소재를 기능성 화장품 분야에 활용하는 연구가 이루어지고 있다. 식물조직배양기술을 이용할 경 우 특정 Cell line의 유지 및 보존, 주기적인 계대배양으로 인 한 오염방지, 인건비 등 경비절감이 가능한 장점이 있다 (Lee et al 2015). 식물조직배양은 식물의 세포 및 기관을 연 중 균일하게 대량 생산하는 가장 보편적인 방법으로, 식물 체로부터 기관, 조직 및 세포를 적출 분리하여 기내에서 영 양분이 함유되어 있는 배지를 이용해 무균 배양함으로써 캘러스(callus)를 단세포의 집단으로 유기하거나 완전한 기 능을 가진 식물체로 재생시키는 기술을 말한다 (Oh et al 2014).…”

Callus Derived from Petals of the Rosa hybrida Breeding Line 15R-12-2 as New Material Useful for Fragrance Production

“…In addition, because essential oil is extracted from fresh flowers harvested from late May to late June, it is affected by various factors such as temperature, humidity, and precipitation. Therefore, it is necessary to develop a system that stably supplies raw materials with uniform ingredients in line with the continuous increase in demand [ 8 ].…”

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“…The development of new varieties and making of ideotype are the result of modifications of plant parts during morphological mutations (Khursheed et al, 2019). Induced mutation is highly effective in enhancing natural genetic resources and has been used in developing improved cultivars of cereals, fruits and other crops (Lee et al, 2002).…”