Marine origin materials on biomaterials and advanced therapies to cartilage tissue engineering and regenerative medicine

Carvalho, Duarte Nuno; Reis, Rui L.; Silva, Tiago H.

doi:10.1039/d1bm00809a

Cited by 16 publications

(14 citation statements)

References 164 publications

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“…Recent study employed a mixture of hyaluronan hydrogel, which they then inserted under the skin of hairless rats. After multiple time points, the implant was subjected to histological, immunohistochemical, and mechanical analyses, compared to control studies, the implantation had good machinability, suggesting that cell and hydrogel mixtures could create like-cartilaginous tissue in vivo [ 25 ].…”

Section: Bioprinting Technologiesmentioning

confidence: 99%

Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Owida

2022

Applied Bionics and Biomechanics

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Tissue engineers have made great strides in the past decade thanks to the advent of three-dimensional (3D) bioprinting technology, which has allowed them to create highly customized biological structures with precise geometric design ability, allowing us to close the gap between manufactured and natural tissues. In this work, we first survey the state-of-the-art methods, cells, and materials for 3D bioprinting. The modern uses of this method in tissue engineering are then briefly discussed. Following this, the main benefits of 3D bioprinting in tissue engineering are outlined in depth, including the ability to rapidly prototype the individualized structure and the ability to engineer with a highly controllable microenvironment. Finally, we offer some predictions for the future of 3D bioprinting in the field of tissue engineering.

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Section: Bioprinting Technologiesmentioning

confidence: 99%

Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Owida

2022

Applied Bionics and Biomechanics

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“…Furthermore, the scaffold should be conducive to the diffusion of nutrients, the discharge of metabolic waste, and should provide a stable external environment for cell growth and differentiation. In addition, the scaffold material should have good shapeability and it should be easy to prepare the tissue model of irregular defects ( Carvalho et al, 2021 ; Wei et al, 2021 ). Currently, sodium alginate and collagen are two of the most widely used hydrogels in cartilage tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, sodium alginate and collagen are two of the most widely used hydrogels in cartilage tissue engineering. When exposed to divalent cations, such as Ca 2+ , Ba 2+ or Sr 2+ , alginate generates a hydrogel that is similar to an extracellular matrix ( Ahmad Raus et al, 2021 ; Carvalho et al, 2021 ). Levato et al (2017) found that employing hydrogel as a bio-scaffold material could accelerate chondrogenesis and inhibit osteogenesis in CSPCs.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Zhang

Qi²,

Chen³

et al. 2023

Front. Bioeng. Biotechnol.

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With the help of biomaterials, cartilage stem/progenitor cells (CSPCs) derived from cartilage tissue present a promising choice for cartilage regeneration. In our previous study, we investigated whether CSPCs could be ideal seeding cells for cartilage tissue regeneration. Biomaterials are fabricated to accelerate tissue regeneration, providing a suitable environment for cell attachment, proliferation, and differentiation. Among the biomaterials used in cartilage regeneration medicine, alginate and collagen are classified as natural biomaterials and are characterized by high biocompatibility, bioactivity, and non-toxic degradation products. However, it is unclear which material would have a competitive advantage in CSPC-based cartilage regeneration in vivo. In the present study, we employed alginate and type Ⅰ collagen as substrates for CSPCs and chondrocytes, which was made control group, to explore a more suitable biomaterials for CSPCs to fabricate tissue-engineered cartilage, in vivo. Hematoxylin and eosin (HE) staining, Safranin O, immunohistochemical assay, and quantitative real-time polymerase chain reaction (qRT-PCR) were used to evaluate the tissue-engineered cartilage in vivo. Compared with the alginate group, collagen enhanced the expression of cartilage-specific genes, such as ACAN, SOX9, and COLII, more markedly. Furthermore, the marker genes of expression, dedifferentiation, and hypertrophy, COLI and COLX, were downregulated in the collagen group. The results demonstrated that collagen as a substrate was superior to alginate in increasing the accumulation of cartilage-like ECM for CSPCs in vivo. In summary, compared with alginate, collagen hydrogel is an effective biomaterial for CSPC-based cartilage regeneration.

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“…Marine microorganisms, as important members of marine organisms, can produce numerous specific active substances in an extreme environment in low temperature, high salt, high pressure, and oligotrophic conditions. They have gained scientific interest due to their potential applications in the pharmaceutical, cosmetic and food industries, for environmental remediation, and astrobiology [ 1 , 2 , 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from Marine Microbes: Source, Structure and Application

Zheng

et al. 2022

Marine Drugs

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The unique living environment of marine microorganisms endows them with the potential to produce novel chemical compounds with various biological activities. Among them, the exopolysaccharides produced by marine microbes are an important factor for them to survive in these extreme environments. Up to now, exopolysaccharides from marine microbes, especially from extremophiles, have attracted more and more attention due to their structural complexity, biodegradability, biological activities, and biocompatibility. With the development of culture and separation methods, an increasing number of novel exopolysaccharides are being found and investigated. Here, the source, structure and biological activities of exopolysaccharides, as well as their potential applications in environmental restoration fields of the last decade are summarized, indicating the commercial potential of these versatile EPS in different areas, such as food, cosmetic, and biomedical industries, and also in environmental remediation.

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Marine origin materials on biomaterials and advanced therapies to cartilage tissue engineering and regenerative medicine

Abstract: The body´s self-repair capacity is limited, including injuries on articular cartilage zones. Over the past few decades, tissue engineering and regenerative medicine (TERM) have focused the studies on the development...

Cited by 16 publications

References 164 publications

Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Comparative study of alginate and type I collagen as biomaterials for cartilage stem/progenitor cells to construct tissue-engineered cartilage in vivo

Exopolysaccharides from Marine Microbes: Source, Structure and Application

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