Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Owida, Hamza Abu

doi:10.1155/2022/2260216

Cited by 5 publications

(5 citation statements)

References 75 publications

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“…Throughout the bioprinting process, the integrity of living cells can be damaged, decreasing cell viability and altering the biological and structural characteristics of the bioprinted TEP. The most common approaches to bioprint a TEP are: (i) inkjet‐based 3D bioprinting, (ii) stereolithographic‐based 3D bioprinting (SLA), (iii) laser 3D bioprinting, and (iv) extrusion‐based bioprinting (EBB) (Abu Owida, 2022; Jose et al, 2016). EBB is the most convenient and scalable bioprinting technique of TEP.…”

Section: Introductionsupporting

confidence: 81%

“…Then, the bioprinter extrudes the bioink using air or mechanical pressure to draw the designed tissue. EBB provides several advantages over other techniques such as: (i) allowing the use of bioinks with higher viscosity which are bioprinted as filaments through the needle and can maintain this shape after their deposition (post‐bioprinting), (ii) offers a greater cell coverage and helps maintaining cell viability of TEP, (iii) greater precision, (iv) faster bioprinting speed, and (v) versatility to bioprint a wider range of biomaterials (Abu Owida, 2022). However, the use of highly bioprintable bioinks is associated to low cell viability recovery.…”

Section: Introductionmentioning

confidence: 99%

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Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds

Martorell

López-Fernández

García-Lizarribar

et al. 2023

Biotech & Bioengineering

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Three dimensional (3D) bioprinting is an emerging technology that enables complex spatial modeling of cell‐based tissue engineering products, whose therapeutic potential in regenerative medicine is enormous. However, its success largely depends on the definition of a bioprintable zone, which is specific for each combination of cell‐loaded hydrogels (or bioinks) and scaffolds, matching the mechanical and biological characteristics of the target tissue to be repaired. Therefore proper adjustment of the bioink formulation requires a compromise between: (i) the maintenance of cellular critical quality attributes (CQA) within a defined range of specifications to cell component, and (ii) the mechanical characteristics of the printed tissue to biofabricate. Herein, we investigated the advantages of using natural hydrogel‐based bioinks to preserve the most relevant CQA in bone tissue regeneration applications, particularly focusing on cell viability and osteogenic potential of multipotent mesenchymal stromal cells (MSCs) displaying tripotency in vitro, and a phenotypic profile of 99.9% CD105+/CD45,− 10.3% HLA‐DR,+ 100.0% CD90,+ and 99.2% CD73+/CD31− expression. Remarkably, hyaluronic acid, fibrin, and gelatin allowed for optimal recovery of viable cells, while preserving MSC's proliferation capacity and osteogenic potency in vitro. This was achieved by providing a 3D structure with a compression module below 8.8 ± 0.5 kPa, given that higher values resulted in cell loss by mechanical stress. Beyond the biocompatibility of naturally occurring polymers, our results highlight the enhanced protection on CQA exerted by bioinks of natural origin (preferably HA, gelatin, and fibrin) on MSC, bone marrow during the 3D bioprinting process, reducing shear stress and offering structural support for proliferation and osteogenic differentiation.

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Section: Introductionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds

Martorell

López-Fernández

García-Lizarribar

et al. 2023

Biotech & Bioengineering

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“…This technique is based on the polymerization of light-sensitive polymers. UV radiation used in conventional stereolithography can be harmful to cells and can trigger mutations [103]. In common, these 3D bioprinting techniques have limitations according to the properties of the bioinks, such as their viscosity, directly impacting the bioprinting quality of the material [100,104,105].…”

Section: D Bioprintingmentioning

confidence: 99%

Trends and Technological Challenges of 3D Bioprinting in Cultured Meat: Technological Prospection

Barbosa,

Correia,

Vieira

et al. 2023

Applied Sciences

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Cultured meat presents a possible alternative to conventional meat products and may be used to address growing food demands attributable to global population growth. Thus, a comprehensive technological prospection of the scientific literature related to cultured meat produced by 3D bioprinting is of great interest to researchers. The purpose of this article is to review and analyze published studies related to the biofabrication of cultured meat using 3D bioprinting techniques. The growing number of related publications in recent years highlights that cultured meat has gained traction in the scientific community. Furthermore, private companies and startups have contributed to advancements in the biofabrication of cultured meat for consumption, illustrating that cultured meat as a conventional meat substitute is already becoming reality. However, like any scientific advance, 3D bioprinting of cultured meat faces challenges involving regulation, acceptance, the selection of ideal biomaterials and cell lines, the replacement of fetal bovine serum (FBS), and attaining a texture and nutritional value similar to those of conventional meat.

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“…The filament is then deposited onto the print plate, in a layer-bylayer manner, based on a 3D computer model. Pneumatic mechanisms, pistons, or screws can be employed to drive the extrusion of the ink through the nozzle (Abu Owida, 2022). Post-extrusion treatments such as light exposure, annealing, or solvent treatment can offer a pathway to trigger physical or chemical crosslinking mechanisms that retain the shape fidelity of the hydrogel-based constructs after printing (GhavamiNejad et al, 2020;Puza and Lienkamp, 2022).…”

Section: Graphical Abstract 1 Introductionmentioning

confidence: 99%

Monitoring the micellar packing of photo-crosslinkable Pluronic F127 dimethacrylate during 3D printing

Bomediano,

da Silva,

Mota-Santiago

et al. 2024

Front. Soft Matter

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Pluronic F127 dimethacrylate (F127-DM) inks exhibit potential for 3D printing biomaterials due to their combined gelation and photo-crosslinking capabilities. Previous studies have explored their application in extrusion 3D printing of medical devices, relying on their long-range micellar ordering to impart mechanical stability, stretchability, and control over drug delivery. However, there is a lack of information regarding the impact of the extrusion and photo-crosslinking processes on the micellar ordering of F127-DM. Herein, we employed in operando 3D printing synchrotron small-angle X-ray scattering (SAXS) to analyze the influence of various printing parameters, including nozzle shape, nozzle size, extrusion rate, ink composition, and photo-crosslinking, on the micellar ordering of F127-DM. Our findings from 2D SAXS patterns indicate orientation of the micellar packing clusters along the flow direction. The nozzle diameter and shape emerged as crucial parameters, leading to increased disruption of long-range ordering. Furthermore, 1D SAXS curves during UV-Vis light exposure and photo-crosslinking revealed a partial preservation of the micellar packing structure, with cluster size reduction, and coexisting with randomly distributed micelles and unimers. These results underscore the importance of in operando synchrotron experiments for the systematic study of 3D printing parameters from a nanostructure perspective.

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Developments and Clinical Applications of Biomimetic Tissue Regeneration using 3D Bioprinting Technique

Cited by 5 publications

References 75 publications

Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds

Preservation of critical quality attributes of mesenchymal stromal cells in 3D bioprinted structures by using natural hydrogel scaffolds

Trends and Technological Challenges of 3D Bioprinting in Cultured Meat: Technological Prospection

Monitoring the micellar packing of photo-crosslinkable Pluronic F127 dimethacrylate during 3D printing

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