Magnetic microparticle-based multiplexed DNA detection with biobarcoded quantum dot probes

Ding, Xiang; Zeng, Guoping; He, Zhibing

doi:10.1016/j.bios.2011.04.051

Cited by 37 publications

(11 citation statements)

References 46 publications

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“…After hybridization, MMPs were magnetically separated from the sample followed by a heating step to release the labels inside the solutions. Afterwards, HPV‐16 and HPV‐18 were detected by simply measuring the FAM and Rox fluorescent signal at concentrations down to 70 and 60 pM, respectively .…”

Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

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Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV‐16 and 18, has been a topic of focus by many researchers. In this systematic review, we highlight the route of development of DNA‐based genosensory detection methods for diagnosis of high risk of HPV precancer. Biosensor detection methods of HPV‐16 and 18 was investigated from 1994 to 2018 using several databases including PubMed, Cochrane Library, Scopus, Google Scholar, SID, and Scientific Information Database. Manual search of references of retrieved articles were also performed. A total of 50 studies were reviewed. By analyzing the most recent developed electrochemical biosensors for the identification of HPV, we observed that the sensor platform fabricated by Wang et al. holds the lowest detection limit reported in the literature for the DNA of HPV‐16. Up to this date, optical, electrochemical, and piezoelectric systems are the main transducers used in the development of biosensors. Among the most sensitive techniques available to study the biorecognition activity of the sensors, we highlight the biosensors based fluorescent, EIS, and QCM. The current systematic review focuses on the sensory diagnostic methods that are being used to detect HPV‐16 and 18 worldwide. Special emphasis is given on the sensory techniques that can diagnosis the individuals with CC. © 2018 BioFactors, 45(2):101–117, 2019

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Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

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“…Then, fluorescein amidite (FAM) and 6-carboxyl-X-rhodamine (Rox) labeled HPV-16 and HPV-18 detection probes were incubated with particles to identify target DNA strands. Magnetic microparticles (MMPs) were also used as capture surfaces for target HPV DNA strands [26]. MMPs functionalized with HPV-16 and HPV-18 capture probe DNA strands were simultaneously mixed with HPV-16 and HPV-18 target DNA and HPV-16 and HPV-18 detection probe DNA strands with the functionalized labels.…”

Section: A Optical Detection Technologiesmentioning

confidence: 99%

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

et al. 2015

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| Human papillomavirus (HPV) has been shown in many studies as a prerequisite for the development of cervical cancer, which is the second most common and predominant form of malignancy among women worldwide, with 270 000 deaths (80% of whom live in developing countries) and 500 000 new cases per year. Early diagnosis of cervical cancer allows patients to be fully treated. Therefore, there is high clinical utility of HPV diagnostics even with binary positive/ negative indication of the presence of HPV in patient samples.Although the Pap smear is widely used for cervical cancer screening, this method still suffers from low sensitivity and specificity. Thus, simple, rapid and inexpensive diagnostic methods are needed to detect the etiology of cervical cancer, i.e., HPV, especially high-risk oncogenic subtypes 16 and 18.Here, we review the existing assays and platforms employed for HPV diagnosis, and highlight recent advances in nanotechnology and microfluidics that potentially enable new approaches for HPV diagnosis.

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“…Nanoparticles are generally exploited by bioconjugation, that is, by the intelligent mixing of the nanostructure with some biological molecules as DNA or RNA oligonucleotides; this conjugation is made to favor the binding of small oligonucleotides that are complementary to HPV DNA. These kinds of bioconjugated systems have demonstrated to differentiate between HPV16 and HPV18 strains mixed in the same sample [101]. Usually utilized as reporters or signal enhancers [52,54], nanoparticles are designed based on their combination with another technique.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Trends in Biosensors for HPV: Identification and Diagnosis

et al. 2015

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The conventional methodologies used for the detection of human papillomavirus (HPV) present actually robust and reproducible advantages. However, at the same time, they involve complex protocols that sometimes are difficult to popularize. Over the first half of XX century, the adequate treatment of complex and delicate processes from a simple instrumental base seemed a fundamental and intrinsic contradiction. However, interdisciplinary trends have allowed the manipulation of tissues, proteins, and nucleic acids through innovative increasingly smaller devices. The proper diagnosis of HPV has seen great advances since biosensor researchers are employing its virus strains as models to study the interactions between the biorecognition element and the transducer. Additionally, all recent improvements and trends that material sciences, biotechnology, and data processing scientists excel for biosensors can be applied for the HPV detection platforms. In this review, we highlight the recent trends on materials, nanomaterials, and transducers for the specific detection and differentiation of HPV strains. The most influential methods for the detection and identification of these papillomaviruses include optical, electrochemical, and piezoelectric transducers; we will visit their sensibility and advantages. Additionally, we highlight the factors that contributed to the increasing importance of these biodevices as potential substitutes to conventional diagnostic methods.

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Magnetic microparticle-based multiplexed DNA detection with biobarcoded quantum dot probes

Cited by 37 publications

References 46 publications

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

Advances in Nanotechnology and Microfluidics for Human Papillomavirus Diagnostics

Trends in Biosensors for HPV: Identification and Diagnosis

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