Liquid Smoke Antimicrobial Test of Cocoa Fruit Peel Against Eschericia Coli and Staphylococcus Aureus Bacteria

Abstract

Cocoa fruit peel is one of the natural wastes that has not been widely used. Cocoa fruit peel is thought to have secondary metabolites that can kill bacteria. The purpose of this study is to determine the antibacterial properties of liquid cocoa peel smoke and, determining minimum inhibitory concentration as well as concentration growth of Escherichia coli and Staphylococcus aureus pathogenic bacteria. This research had been conducted in April to May 2019. Testing of antibacterial activity was Well method. The results of study showed that the liquid smoke of cocoa peel at 10% moisture content was able to inhibit the growth of E.coli and, S.aureus bacteria at the highest level compared to the liquid smoke of brown skin moisture content of 15%, 20% and 25%. The inhibitory zone value in Cocoa fruit peel liquid smoke with moisture content of 10% against E.coli bacteria was 25.1 mm, while S.aureus bacteria had an inhibition zone value of 32.6 mm. E.coli bacteria showed stronger resistance compared to S.aureus. This was indicated by the E.coli inhibition zone was smaller than the S. Aureus inhibition zone. The minimum inhibitory concentration and, the minimum kill concentration in a row were at concentrations of 3.125% and 12.5% for E.coli bacteria.

Komponen Kimia Organik Lima Jenis Asap Cair

“…Asap cair memiliki variasi manfaat di pelbagai bidang. Penelitian sebelumnya telah mencatat dampak positif asap cair terhadap kumbang tanduk (Santoso, 2016), walang sangit (Santoso, 2015), preservasi kayu (Subekti, Fibriana, & Widiyaningrum, 2018), produk/produk turunan karet (Muthawali, 2016;Prasetyowati, Hermanto, & Farizy, 2014;Telaubanua, Wirjosentono, & Eddiyanto, 2013), antimikroba (Budaraga & Putra, 2019), pengawet produk makanan (Fitriarni & Ayuni, 2018;Hutomo, Swastawati, & Rianingsih, 2015;Lala, Pongoh, & Taher, 2017;Swastawati, Cahyono, & Wijayanti, 2017), antibakteri (Mashuni, Kadidae, Jahiding, Dermawan, & Hamid, 2019;Oktarina, Sumpono, & Elvia, 2017;Saputra, Trisatya, Darmawan, Wibisono, & Pari, 2020), produktivitas tanaman (Ahadiyat, Herliana, Widiyawati, & Santoso, 2019;Efendi, Mardhiansyah, & Saulaeman, 2019;Istiqomah & Kusumawati, 2019;Yuningsih, Sampoerno, & Puspita, 2015), agen chelating (A'yuni, et al, 2017), dan anti-oksidan (Budaraga, Susanti, Asnurita, Nurdin, & Ramaiyulis, 2019).…”

“…Asap cair telah dibuktikan efektif melawan E. coli pada konsentrasi 100%, ditandai dengan diameter zona bening yang luas (Oktarina, et al, 2017). Riset lain mencatat dampak serupa terhadap E. coli dan S. aureus (Mashuni et al, 2019), namun E. coli menunjukkan resistensi lebih tinggi dibanding S. aureus (Budaraga & Putra, 2019). Bahkan asap cair dapat digunakan untuk memperkuat efek positif minyak sitronella melawan kedua jenis bakteri tersebut (Saputra et al, 2020).…”

Preparation and Characterization of Liquid Smoke from Wood Sawdust Azadirachta excelsa (Jack) M. Jacobs and its Application as a Natural Rubber Coagulant

“…AE wood contains 46.42% cellulose, 33.16% lignin, and 2.54% extractive substances [2]. Liquid smoke has various applications, including as an antimicrobial [3][4][5], antioxidant [6,7], latex coagulant [8,9], termiticide [10,11] and others. As a latex coagulant, liquid smoke was selected because it can accelerate the coagulation time and eliminate the bad smell of latex.…”

Comparison Study of Ionic Gelation and SNEDDs Method in the Preparation of Cocoa Peel Extract Nanoparticles as Antibacterial Against Klebsiella pneumonia

“…Every ton of dry cocoa beans will produce 10 tons of cocoa peel. If the cocoa peel is not processed, it will cause environmental pollution [1,2]. Wiyono et al [3] have investigated that cocoa peel extract contains 344.6 ± 18.7 g/mL of total flavonoids.…”