Larvicidal activity of Copaifera reticulata ducke oil-resin against Culex quinquefasciatus Say (Diptera: Culicidae)

Abstract

Neotropical Entomology 32(4): 729-732 (2003) Atividade Larvicida do Óleo-Resina de Copaifera reticulata Sobre Culex quinquefasciatus Say (Diptera: Culicidae)RESUMO -A evolução de resistência de Culex quinquefasciatus Say aos inseticidas sintéticos e a modificação da suscetibilidade desse mosquito aos piretróides, estimularam estudos sobre a atividade de plantas inseticidas, como alternativa para o seu controle. Neste trabalho foram realizados ensaios biológicos para verificar a atividade larvicida do óleo-resina de Copaifera reticulata sobre C. quinquefasciatus. Para a realização dos bioensaios o óleo-resina foi solubilizado com dimetilsulfóxido (DMSO), na proporção de 0,4 ml do DMSO para 24,6 ml de água destilada. Para cada estádio, os bioensaios foram realizados em copos descartáveis, contendo 100 ml de solução na qual colocavam-se 20 larvas, com quatro réplicas para ajuste das concentrações letais. PALAVRAS-CHAVE: Inseticida botânico, elefantíase, controle, CulicinaeABSTRACT -The appearance of resistance of Culex quinquefasciatus Say to chemical insecticides and the modification of the susceptibility of this mosquito to pyrethroid has stimulated studies on the activity of plants as alternative for its control. In this study, bioassays were carried out to verify the larvicidal activity of the oil-resin of Copaifera reticulata on C. quinquefasciatus. The bioassays were carried out with the oil dissolved in dimethylsulfoxide (DMSO), at the proportion of 0.4 ml of DMSO to 24.6 ml of distilled water. For each instar, the bioassays were carried out in plastic glasses with 100 ml solution using 20 larvae, wit four replications to adjust the lethal concentrations. The mortality observations were made after 48h exposure of the larvae to the solution. The control was carried out in distilled water and DMSO, with the same number of replications as the bioassays. The experiments were carried out in a biological chamber, controlled at 28 ± 1°C, 80 ± 5% relative humidity and 12h light. The larvae without movement were considered dead. The C. reticulata oil-resin demonstrated larvicide activity for all the C. quinquefasciatus instars. The LC 50 found for the 1 st , 2 nd , 3 rd and 4 th larval instars were: 0.4, 0.9, 39 and 80 ppm, and the LC 99 were 15, 15, 50 and 180 ppm, respectively.

Alterações ultraestruturais em larvas de Aedes aegypti submetidas ao diterpeno labdano, isolado de Copaifera reticulata (Leguminosae), e à uma fração rica em taninos de Magonia pubescens (Sapindaceae)

“…Em busca de novas alternativas para o controle do Aedes aegypti, várias pesquisas vêm sendo desenvolvidas no intuito de encontrar um inseticida eficaz com menor impacto ambiental [11][12][13][14][15][16][17][18][19] .…”

Larvicidal Activity of Lantana Indica and Vitex Negundo on Culex Quinquefasciatus

“…At an interval of 1.5 days, the larvae undergo molting and pass four instar stages. The late 3 rd instar and the early 4 th instar are the best-suited phases for testing the efficacy of the plant extract since they are mature and after which they develop into a pupa [12]. The experiments were performed in a controlled environment.…”

“…Molecular docking was performed using iGEM docking tool a generic docking tool for understanding protein-ligand interaction [12]. This docking protocol has been applied to other targets to find the best docking poses of the ligands.…”

In vitro efficacy of Coriandrum sativum, Lippia sidoides and Copaifera reticulata against Leishmania chagasi

“…C. reticulata oleoresin, commonly called, óleo de Copaíba in Portuguese, belongs to the family Fabaceae. It has been evaluated against Culex quinquefasciatus as well as A. aegypti (SILVA et al, 2003;GERIS et al, 2008) and has proven activity anti-Trypanosoma and anti-Leishmania (MACIEL et al, 2002;. C. sativum, called coentro in Portuguese, belongs to the family Umbelliferae and has antimutagenic (CORTÉS-ESLAVA et al, 2004), antidiabetic (GALLAGHER et al, 2003), diuretic (AISSAOUI et al, 2008), gastroprotective (AL-MOFLEH et al, 2006), anti-fungi (ATANDA et al, 2007), as well as anxiolytic effects (EMAMGHOREISHI et al, 2005).…”