KOMPOSISI KIMIA DAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN KAYU PUTIH (Melaleuca cajuputi) DARI KOTA SINGKAWANG

Abstract

ABSTRAK. Kayu putih (Melaleuca cajuputi) merupakan tumbuhan yang banyak dijumpai di pesisir pantai Kota Singkawang. Tumbuhan ini berpotensi digunakan untuk mengobati diare. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri daun kayu putih yang berasal dariKota Singkawang terhadap bakteri uji Escherichia coli dan Staphylococcus aureus. Metode yang digunakan untuk isolasi minyak atsiri yaitu distilasi uap, sedangkan uji aktivitas antibakteri menggunakan metode difusi sumuran. Hasil isolasi minyak atsiri daun kayu putih menghasilkan rendemen sebesar 0,55%. Analisis GC-MS menghasilkan 26 puncak senyawa dengan 2 senyawa utama yaitu 1,8 sineol sebesar 71,96% dan alpha-terpineol sebesar 11,44%. Hasil uji aktivitas antibakteri minyak atsiri daun kayu putih terhadap bakteri E. coli dan S. aureus menghasilkan aktivitas yang tergolong lemah. Diameter zona hambat terhadap bakteri E. coli dengan konsentrasi 50µg/µl menghasilkan zona sebesar 4,39 mm, sedangkan pada bakteri S. aureus yaitu sebesar 4,62 mm. Kata kunci: Escherichia coli, Melaleuca cajuputi, minyak atsiri daun kayu putih, Singkawang, Staphylococcus aureus ABSTRACT. Eucalyptus (Melaleuca cajuputi), is a plant which often found on the coast of the city of Singkawang, West Kalimantan. This plant is potentially used to treat diarrhea. This study aimed to determine antibacterial activity of eucalyptus leaf from Singkawang city on bacteria Escherichia coli and Staphylococcus aureus. Isolation of the essential oils was using steam distillation, while the analysis of antibacterial activity with well diffusion method. The isolation resulted 0.55% yield. Analysis using GC-MS yielded 26 peak compounds with 2 main compounds namely 1.8 cineol at 71.96% and alpha-terpineol 11.44%. Furthermore, antibacterial activity test of eucalyptus leaf essential oil against E. coli and S. aureus bacteria belongs to the category of low. The diameter of the inhibition zone on bacteria E. coli at a concentration of 50µg/µl obtained 4.39 mm zone, while on S. aureus was 4.62 mm zone.

Antibacterial Effects of Eucalyptus Oil (Melaleuca leucadendra) Made in Lamongan against Escherichia coli Bacteria In vitro Study

“…Similar results were also obtained in another study the inhibition zone diameter for concentrations of 100% (14.77±0.68 mm), 75% (14.07±1.08 mm), 50% (13.23±5.30 mm), 25% (7.87±0.23 mm) [15]. In addition, another research also showed that at a concentration of 25 g/l (2.5%) the diameter of the inhibition zone was 3.44±0.34 mm and at a concentration of 50 g/µl (5%) the diameter of the inhibition zone was 4.39±0.48 mm, which further confirm the result in this study [14].…”

“…In another study an analysis of eucalyptus oil (Melaleuca cajuputi) made in Singkawang, West Kalimantan, Indonesia. It was found that 9 bioactive compounds in eucalyptus oil (Melaleuca cajuputi), the highest concentration compound was 1,8-Cineol (71.96%) [14]. In this study 10 compound was found in eucalyptus oil (Melaleuca leucadendra), the highest concentration compound was 1,8-Cineol (72.30%) which is higher.…”