Antibacterial Effects of Eucalyptus Oil (Melaleuca leucadendra) Made in Lamongan against Escherichia coli Bacteria In vitro Study

Abstract

Diarrhea is a health problem that occurs throughout the world, in Indonesia the prevalence of diarrhea is 11%. Diarrhea is one of the food borne diseases. The most common bacteria that causes diarrhea is Escherichia coli. Diarrhea can be prevented by disinfection using 70% alcohol hand sanitizer, but this material often causes irritation and itching, so alternative materials were needed. One of the potential ingredients is eucalyptus oil (Melaleuca leucadendra) made in Lamongan, because it contains 1,8-Cineol compounds. This study aimed to analyze the antibacterial potential of eucalyptus oil as a solution to health problems. This study was a true experimental study with a posttest-only control group design. Sampling used a simple random sampling technique. Data were obtained by measuring the diameter of the inhibition zone using the diffusion method antibacterial test. The data would be processed using IBM SPSS Statistics 23 software. The results of the measurement of the diameter of the inhibition zone in positive control (31.53 ± 0.6 mm), negative control (0.00 ± 0.00 mm) 20% concentration (19.00 ± 1.17 mm), 30% concentration (21.53 ± 1.33 mm), 40% concentration (24.08 ± 0.28 mm), and 50% concentration (24.76 ± 1.56 mm). Based on the One-Way ANOVA test, it was found that there was a significant difference between the control group and the treatment group (p = 0.000). Based on the Pearson test, it was found that there was a very strong relationship (r = 0.888), positive (r = 0.888) and significant (p = 0.000) between various concentrations of eucalyptus oil (Melaleuca leucadendra) made in Lamongan against Escherichia coli bacteria. There was a significant antibacterial effect of eucalyptus oil (Melaleuca leucadendra) made in Lamongan against Escherichia coli bacteria.

The Potential of Cajuput Oil (Melaleuca leucadendra) Small and Medium Enterprises (SME) in Lamongan against Salmonella Typhi Bacteria

“…7 Cajuput oil produced by Lamongan SME has also been proven to inhibit the growth of Escherichia coli bacteria at concentrations of 20%, 30%, 40%, and 50%. 15 According to Şimşek and Duman, the mechanism of 1,8-cineole in inhibiting bacterial growth is by damaging the structure and integrity of organelles and causing cytoplasm coagulation in bacteria due to the destruction of the bacterial cytoplasmic membrane. 12 1,8-cineole has also been shown to kill Salmonella bacteria by destroying the cell wall and cell membrane, leading to intracellular material leakage and ultimately damaging cell integrity, resulting in bacterial cell death.…”

Antibacterial Activity of Cajeput Oil (Melaleuca leucadendra) from UMKM Lamongan against Pseudomonas aeruginosa

“…The quality of the cajeput oil used in this study was analyzed based on the Indonesian National Standard No. 3954 of 2014, indicating that cajeput oil (Melaleuca leucadendra) produced in Lamongan has superior quality with a concentration of 72.30% of 1,8-cineole content measured by gas chromatography [9]. The 1,8-cineole content present in this cajeput oil can inhibit bacterial growth by hindering cell wall formation, damaging cell membranes, inhibiting enzyme function, and destroying the genetic material (DNA/RNA) within bacteria [8].…”

“…In that study, the inhibition zone began to appear at a concentration of 20% and the largest diameter at a concentration of 50%. This shows that there is an antibacterial effect of eucalyptus oil produced by Lamongan on Escherichia coli bacteria [9]. Based on previous research, researchers chose eucalyptus oil made in Lamongan which is extracted from melaleuca leucadendra so that it can be used as supporting data in developing treatments for Pseudomonas aeruginosa infections.…”