Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat

Istia'nah, Dina; Utami, Ulfah; Barizi, Ahmad Barizi

doi:10.26740/jrba.v2n1.p11-17

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…This is in line with the opinion of Firliani et al, (2013), that biotic and abiotic conditions in the hot spring environment are very influential. According to Istia et al, (2020), one way to produce amylase enzymes using microorganisms, control of environmental factors is very important because microorganisms themselves are influenced by several things, namely temperature, incubation period and pH. The utilization of bacteria in producing amylase enzymes has an important role in the industrial field.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort

Angio,

Abdul,

Kumaji

et al. 2024

JPBN

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The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria

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Section: Discussionmentioning

confidence: 99%

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort

Angio,

Abdul,

Kumaji

et al. 2024

JPBN

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“…Bakteri amilolitik yang kontak langsung dengan substrat amilum akan memutuskan ikatan glikosida pada senyawa polimer amilum menjadi monomer yang lebih sederhana seperti maltosa, dekstrin, dan glukosa (Nurfajriyah et al, 2017). Produksi dan aktivitas enzim amilase oleh bakteri dipengaruhi oleh beberapa faktor di antaranya suhu, pH, waktu, jenis bakteri, jenis dan konsentrasi substrat (Arun & Sivashanmugam., 2017;Istia'nah et al, 2020). Amilase bermanfaat dalam industri makanan, tekstil, kertas, dan detergen (de Souza & de Oliveira e Magalhães, 2010).…”

Section: Pendahuluanunclassified

Untitled

Rahmawati¹,

Oedjijono²,

Ryandini³

2022

bioe

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Penelitian ini bertujuan untuk mengetahui kemampuan isolat bakteri sedimen mangrove dalam menghasilkan enzim amilase, mengetahui pH dan suhu optimum aktivitas amilase yang dihasilkan, dan mengidentifikasibakteri penghasil amilase. Penelitian dilakukan dengan metode survei. Tahap penelitian meliputi, skrining bakteri amilolitik, pembuatan kurva tumbuh, produksi amilase, optimasi aktivitas amilolitik pada variasi suhu (35ºC; 36ºC; 37ºC; 38ºC) dan pH (4,5; 5; 5,5; 6) serta karakterisasi isolat amilolitik. Parameter yang diukur yaitu indeks amilolitik, unit aktivitas enzim amilase, total unit bakteri, dan identitas bakteri amilolitik asal sedimen mangrove. Analisis data dilakukan secara deskriptif. Hasil pengukuran indeks amilolitik tertinggi ditunjukkan oleh isolat LG113 dengan nilai indeks amilolitik 9,86 mm dari 10 isolat amilolitik asal sedimen mangrove. Suhu optimum aktivitas amilase adalah 37ºC yaitu sebesar 2,13 U/mL dan pH optimum adalah 6 yaitu sebesar 2,14 U/mL. Jumlah total sel bakteri amilolitik padaakhir masa produksi adalah 1,94x1013 CFU/mL. Identitas isolat bakteri amilolitik asal sedimen mangrove pantai Logending termasuk anggota genus Bacillus.

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“…Hasil konversi dari pengukuran absorbansi tersebut dapat dilihat pada Tabel 4. Berdasarkan penelitian [12] tentang karakterisasi enzim amilase dari bakteri B.megaterium pada variasi suhu, pH dan konsentrasi substrat, dari hasil penelitian tersebut didapatkan hasil bahwa suhu optimum bakteri B.megaterium dalam memproduksi enzim amilase pada suhu 37 o C dengan aktivitas amilase sebesar 1,279 U/mL. Karakterisasi enzim yang kedua, dilihat dari pH optimum bakteri dalam memproduksi enzim amilase, didapatkan hasil bahwa pH optimum bakteri B. megaterium dalam memproduksi enzim amilase yakni pada pH 5,0 dengan aktivitas enzim sebesar 1,241 U/mL.…”

Section: Produksi Enzim Amilase Bakteri Amilolitikunclassified

“…Enzim amilase yang dihasilkan oleh bakteri B. paramycoides dan E. cloacae relatif lebih sedikit dikarenakan tidak adanya perlakuan kontrol terhadap pH maupun suhu dan konsentrasi substrat. Pada penelitian oleh [12] pengamatan aktivitas enzim selama 140 jam sedangkan penelitian ini hanya selama 24 jam karena adanya keterbatasan waktu.…”

Section: Produksi Enzim Amilase Bakteri Amilolitikunclassified

ABILITY OF AMILOLYTIC BACTERIA (Bacillus paramycoides and Enterobacter cloacae) IN DEGRADING ORGANIC MATERIALS OF MANGROVE LITTLE

Putri

Nursyirwani

Feliatra

2021

ajoas

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This study aims to find out that Bacillus paramycoides and Enterobacter cloacae bacteria can produce amylase enzymes and have the ability to degrade organic matter, especially mangrove litter. From this study it was found that the optimal growth of B.paramycoides and E. cloacae bacteria occurred at 12th hour. The results of measurements and calculations of absorbance values at 630 10.238 x 108 cells/mL (B. paramycoides) and 12.030 x 108 cells/mL (E. cloacae) using the spectrophotometric method. Meanwhile, with the TPC method at 12 hours, the number of bacterial cells was 2.08 x 108 CFU's/ml (B. paramycoides) and 2.44 x 108 CFU's/ml (E. cloacae). The ability to produce the largest amylolytic bacterial amylase enzyme also occurred at 12 hours as much as 0.306 mg/mL (B.paramycoides) with an increase of 0.046 mg/mL and 0.243 mg/mL (E. cloacae) with an increase of 0.028 mg/mL. The bacteria that have the highest amylase enzyme ability is E.cloacae as evidenced by the diameter of the clear zone of 10.10 mm. Testing the ability of amylolytic bacteria in degrading mangrove litter was carried out by adding amylase enzyme as much as 0%, 50% and 100%. Amylolytic bacteria can degrade organic matter by hydrolyzing starch contained in mangrove litter. The most degraded starch content was in the 100% enzyme treatment, which was 1.021 mg/mL (B. paramycoides) and 1.189 mg/mL (E.cloacae).

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Karakterisasi Enzim Amilase dari Bakteri Bacillus megaterium pada Variasi Suhu, pH dan Konsentrasi Substrat

Cited by 4 publications

References 12 publications

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort

Untitled

ABILITY OF AMILOLYTIC BACTERIA (Bacillus paramycoides and Enterobacter cloacae) IN DEGRADING ORGANIC MATERIALS OF MANGROVE LITTLE

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