Bacillus megaterium merupakan salah satu mikroorganisme potensial yang dapat menghasilkan enzim amilase. Karakterisasi enzim amilase dapat membantu mengetahui kondisi optimum enzim saat bekerja. Penelitian ini bertujuan untuk mengetahui karakteristik enzim amilase yang dihasilkan oleh Bacillus megaterium pada variasi suhu, pH dan konsentrasi substrat. Karakterisasi enzim ditentukan dengan menguji aktivitas enzim pada 3 variasi yakni variasi suhu dengan menggunakan pH netral, variasi pH yang dilakukan pada kondisi suhu optimum hasil perlakuan sebelumnya dan variasi konsentrasi substrat YPSs yang dilakukan pada kondisi suhu dan pH optimum hasil perlakuan sebelumnya. Data aktivitas enzim amilase dianalisis secara deskriptif dengan menentukan nilai aktivitas enzim amilase tertinggi pada setiap perlakuan. Hasil penelitian ini menunjukkan bahwa Bacillus megaterium mampu menghasilkan enzim amilase. Indeks Aktivitas Amilase (IAA) Bacillus megaterium sebesar 2,35 mm. Secara kuantitatif aktivitas enzim amilase ditentukan dengan metode DNS yang untuk mengukur kadar gula reduksi yang diproduksi oleh mikrob dengan tingkat ketelitian yang tinggi. Ekstrak kasar enzim amilase yang diproduksi oleh Bacillus megaterium memiliki karakteristik pada suhu 37oC dengan aktivitas enzim sebesar 1,279 U/mL, sedangkan karakteristik pH berada pada pH 5,0 dengan aktivitas enzim sebesar 1,241 U/mL dan karakteristik konsentrasi substrat berada pada konsentrasi 1,50% dengan aktivitas enzim sebesar 0,548 U/mL.
A lecturer (dosen) is a teacher at higher learning institutions. Their very existence, roles and functions are an undeniable necessity. There will be no education without the presence of a lecturer. A lecturer is the decisive factor in determining the direction and systematics of instructions from the curriculum,facilities,patterns, and even the efforts on how students should study well to gain access to the knowledge and values of Therefore, a lecturer's duty is noble but heavy. But then, what strategy should be used to motivate a lecturer to implement that noble duty? How can a higher learning institution or university create a mature and independent person? What educational system should a university adopt so as not to create people who only grow older but also those who grow up? What significant approach should be used to solve the problem concerning the current ethics of professionalism? It is deemed necesszy that these problems should receive proper attention from the administrators, managers, and supervisors of the educational system at higher learning institutions or unitersities. This ankle will provide answers to the above problems.
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