ISSR variations in eggplant (Solanum melongena L.) and related Solanum species

Isshiki, Shiro; Iwata, Naoyuki; Khan, Md. Mizanur Rahim

doi:10.1016/j.scienta.2008.04.003

Cited by 80 publications

(65 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This observation is in agreement with the findings of Mace et al (1999), andIsshiki et al (2008). These workers used AFLP markers to determine the taxonomic position of S. dasyphyllum and S. macrocarpon both of series Macrocarpa outside section Melongena which comprises S. melongena.…”

Section: Discussionsupporting

confidence: 89%

“…one sample each of S. nigrum and S. scabrum occurring in both clusters III and V. Hammond (1979) stated that biosystematics and evolutionary studies have for long time, and for the most part, considered the morphological features of the mature organism. This observation is evident in earlier works on Solanum taxonomy such as that of Isshiki et al (2008), Karihaloo and Rai (1995), and Oyelana and Ugborogho (2008). Unfortunately, these and many other studies based on morphological features have not totally resolved the difficulties associated with Solanum taxonomy.…”

Section: Resultssupporting

confidence: 59%

“…The advent of molecular biology has revolutionized the field of plant systematics and has been used successfully in phylogenetic relationships at all taxonomic levels (Bohs, 2005) as well as in DNA fingerprinting of plant genomes (Cervera et al, 1998) and in genetic diversity studies (Isshiki et al, 2008;Fory et al, 2010). The use of molecular techniques in genetic diversity studies is supported by the finding that evolutionary forces such as natural selection and genetic drift produce divergent phylogenetic branching which can be recognized because the molecular sequences, on which they are based, share a common ancestor (Singh et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

Sifau¹,

Ogunkanmi²,

Adekoya³

et al. 2014

Int. J. Genet. Mol. Biol.

View full text Add to dashboard Cite

Solanum L., the largest genus of the Solanaceae family, vary morphologically, is diverse in number and is ecogeographically distributed. In Nigeria, previous studies had focused mainly on chromosome morphology, genome description and medicinal values, which are insufficient for genetic affinities. This study used four highly polymorphic random amplified polymorphic DNA primers to describe both the genetic relatedness and variability among 25 accessions of eggplant from Southwestern Nigeria. At a truncated line of 65%, five clusters and two ungrouped samples are distinguishable from the dendrogram. The data reveals that Solanum dasyphyllum Schum. & Thonn. is more closely related to Solanum macrocarpon L. than to Solanum melongena L. The relatedness between Solanum incanum L. and Solanum melongena, a probability of being progenitors from a common ancestral lineage was also shown. Occurrence of Solanum scabrum L. and Solanum nigrum L. in the same clusters different from S. melongena, is an indication of distant relatedness to S. melongena but close relatedness between them. High level of polymorphism was observed in this study going by the coefficient of variation which exhibited a good separation from a conserved region of the genome. This study, therefore, reveals a wide and diverse genetic base in Nigerian eggplant Solanum.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Resultssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

Sifau¹,

Ogunkanmi²,

Adekoya³

et al. 2014

Int. J. Genet. Mol. Biol.

View full text Add to dashboard Cite

show abstract

“…A set of 100 ISSR primers (Isshiki et al, 2008) was synthesized according to the sequences obtained from the University of British Columbia, Canada. All 100 primers were screened for their amplification efficiency using six representative samples.…”

Section: Pcr Amplificationmentioning

confidence: 99%

Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China

Yu¹,

Wang²,

Peng³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Eucommia ulmoides Oliver, one of the tertiary relict species found only in China, is the only extant species of Eucommiaceae. Using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers, we studied the genetic diversity and population genetic structure of 187 accessions from 17 E. ulmoides populations throughout its main distribution in China. A total of 65 bands were amplified using eight ISSR primers, of which 50 bands (76.9%) were polymorphic. Meanwhile, another 244 bands were observed using eight SRAP primer combinations and 163 (66.8%) of these were polymorphic. The analysis of molecular variation (AMOVA) indicated that 88.8 and 92.4% of the total variation resided within populations based on ISSR and SRAP analysis, respectively. Moreover, we found that the E. ulmoides populations were clustered into six distinct groups using ISSR and SRAP markers via the unweighted pair-group method (UPGMA). Furthermore, STRUCTURE analysis showed that these 17 populations could be classified into four groups using ISSR markers, but only two groups using SRAP markers. No 2471-2483 (2015) significant relevancy was observed between genetic and geographic distances among the sampled populations. The results of this study support the view that exchange of seeds among local farmers plays an important role in shaping the present genetic distribution pattern. "Core collection" is suggested for genetic diversity conservation of E. ulmoides in China.

show abstract

“…O marcador molecular ISSR tem se mostrado uma poderosa ferramenta para análise da diversidade genética, bem como para a caracterização de acessos e cultivares de diversas espécies, como o cacau e espécies do gênero Solanum (Charters & Wilkinson, 2000;Isshiki et al, 2008). Por se tratar de um marcador multiloco que não requer conhecimento prévio do DNA a ser avaliado (Gupta et al, 1994), o ISSR é uma técnica de baixo custo, de fácil uso e de grande reprodutibilidade (Matthews et al, 1999).…”

Section: Introductionunclassified

Variabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSR

Silva

Alves²,

Martins

et al. 2011

Pesq. agropec. bras.

View full text Add to dashboard Cite

Genetic variation among accessions of the genus Manihot by ISSR markersAbstract -The objective of this work was to evaluate the genetic diversity within and among accessions of Manihot by using ISSR markers. Five species and two varieties of Manihot, besides two species of the genus Croton, used as the out-group, were analyzed by using 20 oligonucleotide (Olii) ISSR UBC primers. To analyze the similarity index between species and accessions, the Jaccard and simple matching coefficients were used. The 20 Olii tested were highly polymorphic in all species analyzed, and 89.7% of the loci were polymorphic. A higher genetic variability is observed among different species of Manihot, such as M. dichotoma var. undulata and M. caerulescens, than among individuals of the same species, such as M. dichotoma and M. dichotoma var. undulata.

show abstract

ISSR variations in eggplant (Solanum melongena L.) and related Solanum species

Cited by 80 publications

References 26 publications

Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

Partitioning and distribution of random amplified polymorphic DNA (RAPD) variation among eggplant Solanum L. in Southwest Nigeria

Genetic diversity and population structure of Eucommia ulmoides Oliver, an endangered medicinal plant in China

Variabilidade genética entre acessos do gênero Manihot por meio de marcadores moleculares ISSR

Contact Info

Product

Resources

About