ISSR Markers as a Tool for the Assessment of Genetic Diversity in Passiflora

Abstract

Genetic variation among sweet, purple, and yellow passion fruit accessions was assessed using inter-simple sequence repeat (ISSR) markers. Eighteen ISSR primers were used to evaluate 45 accessions. The number of polymorphic bands per primer varied from 4 to 22, with 12.4 bands per primer on average. Nei's genetic distance between accessions ranged from 0.04 to 0.35. Clustering using the neighbor-joining method resulted in the formation of 11 major clusters. It was not possible to classify the accessions according to their geographic origin, showing that there is no structure in the gene bank. The overall mean Shannon-Weaver diversity index was 0.32, indicating good resolution of genetic diversity in passion fruit germplasm using ISSR markers. Our results indicate that ISSR can be useful for genetic diversity studies, to provide practical information for parental selection and to assist breeding and conservation strategies.

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

“…A high polymorphism indicating the high-genetic variability of the Passiflora genus by RAPD markers has already been reported by Bellon et al (2009Bellon et al ( , 2014, Viana et al (2010), Castro et al (2011), andCerqueira-Silva et al (2012). This fact have been verified also by Santos et al (2011b), Costa et al (2012), and Sousa et al (2015) by using ISSR markers.…”

Genetic variability assessment in the genus Passiflora by SSR marker

“…Out of these, the primer A08FP1 amplified in all species (Table 4). Aiming to confirm the presence of hybrids in the cross between P. sublanceolata and P. foetida L., Santos et al (2011) used the same primer, which was polymorphic for the parents, whose progeny presented bands belonging to both parents. This indicates that the primer in question is highly polymorphic and can be used in studies with species on the genus Passiflora.…”

“…However, no relationship was observed among the species. Santos et al (2011) found enormous genetic variability while using ISSR markers to access the variability of P. edulis from different sources.…”

“…Thus, highly informative content can be generated (Schlötterer, 2004). Comparative studies on Passiflora have been very successful when using Randomly Amplified Polymorphic (RAPD) molecular markers (Viana et al, 2003;Pérez et al, 2007;Viana et al, 2010), inter-simple sequence repeat (ISSR) (Santos et al, 2011), and chloroplast genes (Yockteng and Nadot, 2004). However, there are only a few studies on the use of microsatellite markers for the characterization of Passiflora involving several species.…”

Avaliação de recursos genéticos de maracujazeiro‑amarelo

“…O avanço da biotecnologia permitiu o desenvolvimento e o uso de ferramentas moleculares para a caracterização do germoplasma de Passiflora (Oliveira et al, 2005;Paula et al, 2010;Santos et al, 2011), embora muitos desses trabalhos tenham focado apenas na determinação da variabilidade genética e não na formação de um banco de dados e na sua utilização em cruzamentos intra e interespecíficos. Os resultados de algumas dessas caracterizações indicam baixa variabilidade em variedades comerciais de maracujazeiro-amarelo, e ampla variação em outras espécies, com base em marcadores RAPD (Viana et al, 2003).…”