Investigation of antioxidant, antibacterial, antidiabetic, and cytotoxicity potential of silver nanoparticles synthesized using the outer peel extract of Ananas comosus (L.)

Abstract

Currently, green nanotechnology-based approaches using waste materials from food have been accepted as an environmentally friendly and cost-effective approach with various biomedical applications. In the current study, AgNPs were synthesized using the outer peel extract of the fruit Ananas comosus (AC), which is a food waste material. Characterization was done using UV–visible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analyses. The formation of AgNPs has confirmed through UV–visible spectroscopy (at 485 nm) by the change of color owing to surface Plasmon resonance. Based on the XRD pattern, the crystalline property of AgNPs was established. The functional group existing in AC outer peel extract accountable for the reduction of Ag + ion and the stabilization of AC-AgNPs was investigated through FT-IR. The morphological structures and elemental composition was determined by SEM and EDX analysis. With the growing application of AgNPs in biomedical perspectives, the biosynthesized AC-AgNPs were evaluated for their antioxidative, antidiabetic, and cytotoxic potential against HepG 2 cells along with their antibacterial potential. The results showed that AC-AgNPs are extremely effective with high antidiabetic potential at a very low concentration as well as it exhibited higher cytotoxic activity against the HepG 2 cancer cells in a dose-dependent manner. It also exhibited potential antioxidant activity and moderate antibacterial activity against the four tested foodborne pathogenic bacteria. Overall, the results highlight the effectiveness and potential applications of AC-AgNPs in biomedical fields such as in the treatment of acute illnesses as well as in drug formulation for treating various diseases such as cancer and diabetes. Further, it has applications in wound dressing or in treating bacterial related diseases.

Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability

“…AgNPs demonstrated a slightly higher MIC and MBC values of 50 and 100 mg ml À1 respectively, against L. monocytogenes. 36 Moreover, a similar observation was recorded earlier where AgNPs synthesized from the aqueous leaf extract of Murraya koenigii exhibited MICs as 32, 16, and 64 mg ml À1 against methicillin-resistant S. aureus (MRSA), E. coli, and ESbLproducing E. coli. 12 In another study, AgNPs synthesized from the extract of Phyla dulcis were found effective against E. coli O157:H7, drug-resistant S. typhimurium, L. monocytogenes and S. aureus.…”

Synthesis of Biogenic Silver Nanoparticles (AgCl-NPs) Using a Pulicaria vulgaris Gaertn. Aerial Part Extract and Their Application as Antibacterial, Antifungal and Antioxidant Agents

“…A mixture of dried AgCl-NPs and KBr was ground into a fine powder and pressed into a pellet. Using a Nicolet 800 FTIR spectrometer (Nicolet, Madison, USA), absorption spectra of AgCl-NPs included in pellets were acquired at wavelengths ranging from 4000 to 400 cm −1 at resolution of 4 cm −1 , as described by Das et al [24].…”

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

“…It is noteworthy to mention that traditional chemical reductants implemented in AgNPs synthesis, such as hydrazine, dimethyl hydrazine, NaBH 4 , etc., are environmentally hazardous and will bring potential environmental risks. However, numerous studies have reported the utilization of medicinal plants to synthesize and stabilize metallic nanoparticles, very particularly AgNPs, via the green synthesis method; a method considered to be cost-effective, environment-friendly and easily scaled up for large-scale synthesis of nanoparticles [22][23][24] . Furthermore, this method does not require high pressure, temperature, and energy.…”