Synthesis of Biogenic Silver Nanoparticles (AgCl-NPs) Using a Pulicaria vulgaris Gaertn. Aerial Part Extract and Their Application as Antibacterial, Antifungal and Antioxidant Agents

Abstract

In this study, very simple and fast one-step synthesis of biogenic silver chloride nanoparticles (AgCl-NPs) using a Pulicaria vulgaris Gaertn. aerial part extract from an aqueous solution of silver nitrate at room temperature is proposed. The proceedings of the reaction were investigated by UV–Vis spectroscopy. AgCl-NPs were characterized using X-ray diffraction spectroscopy (XRD), Fourier-transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). Antibacterial and antifungal activities of these nanoparticles were evaluated by disk diffusion and microdilution methods against Staphylococcus aureus, Escherichia coli, Candida albicans, and C. glabrata. In addition, the antioxidant activity of the synthesized AgCl-NPs was determined by the DPPH radical scavenging assay. The antimicrobial test confirmed the bactericidal activity of biosynthesized AgCl-NPs against Gram-positive and Gram-negative bacteria. They also exhibited good antifungal activities with minimum inhibitory concentration (MIC) values ranging from 40 to 60 µg/mL against Candida glabrata and Candida albicans, respectively. In addition, biosynthesized AgCl-NPs were established to have remarkable antioxidant activity. All this pointed out that the proposed new biosynthesis approach resulted in production of AgCl-NPs with convenient biomedical applications.

Phytofabrication of Silver Nanoparticles (AgNPs) with Pharmaceutical Capabilities Using Otostegia persica (Burm.) Boiss. Leaf Extract

“…The AgNPs were synthesized as described previously [ 2 , 3 ]. In brief, the ethanolic O. persica leaf extract was mixed with a 1 mmol L −1 aqueous solution of silver nitrate (AgNO 3 ) (Merck, Darmstadt, Germany) at a 1:9 ratio.…”

“…For FTIR spectroscopy, a Nicolet 800 FTIR spectrometer (Nicolet, Madison, WI, USA) was used. In this case, the biomolecules present in the ethanolic O. persica leaf extract, involved in the reduction of Ag(I) ions, were identified in the frequency range of 500 to 4000 cm −1 according to the KBr pellet method as described earlier [ 2 , 3 ]. The dried O. persica leaf extract and the dried (and early separated and cleaned) AgNPs were applied as the samples.…”

“…Nanobiotechnology as an interdisciplinary field is rapidly growing and finds useful applications in physics, chemistry, biology, and biomedicine [ 1 , 2 ]. In particular, various plants and microorganisms have received great attention as helpful resources for the synthesis of nanomaterials [ 3 ]. Nanoparticles produced using bacteria-, fungi-, and plants-derived media are more attractive than those obtained with different chemical agents because they are nontoxic, ecofriendly, and safe, especially when considering their potential use in food products and medicines [ 4 ].…”

“…Nanoparticles produced using bacteria-, fungi-, and plants-derived media are more attractive than those obtained with different chemical agents because they are nontoxic, ecofriendly, and safe, especially when considering their potential use in food products and medicines [ 4 ]. Although several pathways were developed for the biosynthesis of metal nanoparticles from corresponding metal salts precursors, the most frugal option is to use plant extracts, which is inexpensive, simple, and fast because it has a one-step character and gives products practically ready to use [ 3 , 5 ]. In the latter case, plant extracts act both as reducing agents and as stabilizers of resultant nanoparticles.…”

Magnetic Nanoparticles of Fe₃O₄ Biosynthesized by <em>Cnicus Benedictus</em> Extract: Photocatalytic Study of Organic Dye Degradation and Antibacterial Behavior

“…In this sense, the new directrix for the synthesis of Fe3O4 nanoparticles is focused on the green chemical. Organic and biocompatibles molecules such as vitamin B12, glucose cellulose, amino acid and even plant extracts has been employed as stabilizing and/or reducing agents [16][17][18][19][20] . It is important to emphasize that the search for new alternatives that promote sustainable chemistry has become a priority issue.…”