Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Abstract

To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.activities of porous Ag nanofilm were investigated. We anticipate that the Ag nanofilm wound dressing with free radical scavenging capacity would enhance the wound healing process. Moreover, this study proposed a low-cost and facile method for preparation of Ag nanofilm as a potential wound dressing. Materials and MethodsMaterials. Methanol (CH 3 OH, 99.9%), silver nitrate (AgNO 3 ), nutrient agar, ferric chloride hexahydrate (FeCl 3 , 6H 2 O), iron (II) sulfate tetrahydrate (FeSO 4 .4H 2 O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco's Modified Eagle's Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa 38 and Staphylococcus aureus 39 bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.Extract preparation. T.polium is a wild-growing...

Development of electrospun keratin/coenzyme Q10/poly vinyl alcohol nanofibrous scaffold containing mupirocin as potential dressing for infected wounds

“…Increase in the antioxidant content of the scaffolds was also attributed to the presence of CoQ10 in the fibers. This is in consonance with Bardania et al [29], who studied the antioxidant and antimicrobial activity of silver ion nanoparticles for wound healing applications [29][30][31]. The DPPH scavenging activity of CoQ10 within the scaffolds was not affected by electrospinning process and did not affect the antioxidant activity of the scaffolds; hence, the presence of CoQ10 offered protection to the cells due to the antioxidant properties [30], leading to growth of the keratinocytes used in the study.…”

Facile green synthesis of silver nanoparticles using Mangifera indica seed aqueous extract and its antimicrobial, antioxidant and cytotoxic potential (3-in-1 system)

“…Concentration dependent increase in ABTS radical scavenging activity is reported by Muniyappan and Nagarajan [46] and Moteriya and Chanda [47]. AgNPs synthesised using Teucrium polium extract showed DPPH and FRAP antioxidant activities [48]. Thus, it can be concluded, that green synthesised AgNPs show antioxidant activity in various antioxidant assays.…”

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