Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability

Al‐Shabib, Nasser Abdulatif; Husain, Fohad Mabood; Nadeem, Mohammad; Khan, Mohd Shahnawaz; Al‐Qurainy, Fahad; Alyousef, Abdullah A.; Arshad, Muhammad; Khan, Altaf; Khan, Javed Masood; Alam, Pravej; Albalawi, Thamer; Shahzad, Syed Ali

doi:10.1039/d0ra04587j

Cited by 15 publications

(8 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Highest decrease of 58% and 64% in the swarming behavior of L. monocytogenes and S. marcescens respectively, was recorded as compared to their untreated controls. Our results find support from the observations made with sub-MICs of AgNPs, ZnO nanoparticles and ZnO-xanthan gum nanocomposite, wherein a significant drop in the swarming motility behavior was recorded in test pathogens [ 49 , 56 , 57 , 58 ].…”

Section: Resultssupporting

confidence: 89%

“…Exopolysaccharides (EPS) are an important constituent of the bacterial biofilm as they have a vital role in the maintenance of the 3D biofilm architecture and providing resistance against the action of antimicrobials and immune responses [ 48 ]. Several studies have highlighted that interference with EPS production leads to disturbed biofilm architecture and eventually reduces the development of resistance against antimicrobials [ 49 , 50 , 51 ]. In light of this, TA-AuNPs were evaluated for their ability to inhibit EPS production in S. marcescens as well as L. monocytogenes .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

et al. 2021

Self Cite

View full text Add to dashboard Cite

Green synthesis of metal nanoparticles using plant extracts as capping and reducing agents for the biomedical applications has received considerable attention. Moreover, emergence and spread of multidrug resistance among bacterial pathogens has become a major health concern and lookout for novel alternative effective drugs has gained momentum. In current study, we synthesized gold nanoparticles using the seed extract of Trachyspermum ammi (TA-AuNPs), assessed its efficacy against drug resistant biofilms of Listeria monocytogenes and Serratia marcescens, and evaluated its anticancer potential against HepG2 cancer cell lines. Microwave-assisted green synthesis of gold nanoparticles was carried out and characterization was done using UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Most nanoparticles were observed as spherical and spheroidal with few anisotropies with an average crystalline size of 16.63 nm. Synthesized TA-AuNPs demonstrated significant biofilm inhibitory activity against L. monocytogenes (73%) as well as S. marcescens (81%). Exopolysaccharide (EPS), motility, and CSH, key elements that facilitate the formation and maintenance of biofilm were also inhibited significantly at the tested sub-minimum inhibitory concentrations (sub-MICs). Further, TA-AuNPs effectively obliterated preformed mature biofilms of S. marcescens and L. monocytogenes by 64% and 58%, respectively. Induction of intracellular ROS production in TA-AuNPs treated bacterial cells could be the plausible mechanism for the reduced biofilm formation in test pathogens. Administration of TA-AuNPs resulted in the arrest of cellular proliferation in a concentration-dependent manner. TA-AuNPs decrease the intracellular GSH in HepG2 cancer cell lines, cells become more prone to ROS generation, hence induce apoptosis. Thus, this work proposes a new eco-friendly and rapid approach for fabricating NPs which can be exploited for multifarious biomedical applications.

show abstract

Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The formation of these AgCl particles could be due to the interaction of the NaCl content of nutrient broth with silver ions, which resulted in formation of AgCl-NPs. However, synthetic yield is involved in components of secondary metabolites secreted by microorganisms and synthesis mechanisms of AgCl-NPs [ 27 ]. In this study, among the nine bacterial strains, only Bacillus mojavensis IMA13 from rhizospheric soil of eggplants indicated the formation of Ag/AgClNPs after incubation for 48 h ( Figure 1 b).…”

Section: Discussionmentioning

confidence: 99%

“…The effect of synthesized AgCl-NPs solution on the swarming motility of cells of strain YY06 was examined in the LB broth supplemented with 0.7% ( w / v ) agar [ 53 ], and on swimming motility of the test bacteria in LB broth containing 0.3% agar [ 27 ]. Briefly, 5, 10, and 20 µg/mL −1 of synthesized AgCl-NP solution was mixed with both all LB media at 45 °C in separate petri plates, and 30 µL of the CFCS or ddH 2 O was used as controls.…”

Section: Methodsmentioning

confidence: 99%

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Alamer

Tomah

et al. 2021

Molecules

View full text Add to dashboard Cite

Ralstonia solanacearum is the most destructive pathogen, causing bacterial wilt disease of eggplant. The present study aimed to develop green synthesis and characterization of silver chloride nanoparticles (AgCl-NPs) by using a native bacterial strain and subsequent evaluation of their antibacterial activity against R. solanacearum. Here, a total of 10 bacterial strains were selected for the biosynthesis of AgCl-NPs. Among them, the highest yield occurred in the synthesis of AgCl-NPs using a cell-free aqueous filtrate of strain IMA13. Ultrastructural observation revealed that the AgCl-NPs were spherical and oval with smooth surfaces and 5–35 nm sizes. XRD analysis studies revealed that these particles contained face-centered cubic crystallites of metallic Ag and AgCl. Moreover, FTIR analysis showed the presence of capping proteins, carbohydrates, lipids, and lipopeptide compounds and crystalline structure of AgCl-NPs. On the basis of phylogenetic analysis using a combination of six gene sequences (16S, gyrA, rpoB, purH, polC, and groEL), we identified strain IMA13 as Bacillus mojavensis. Three kinds of lipopeptide compounds, namely, bacillomycin D, iturin, and fengycin, forming cell-free supernatant produced by strain IAM13, were identified by MALDI-TOF mass spectrometry. Biogenic AgCl-NPs showed substantial antibacterial activity against R. solanacearum at a concentration of 20 µg/mL−1. Motility assays showed that the AgCl-NPs significantly inhibited the swarming and swimming motility (61.4 and 55.8%) against R. solanacearum. Moreover, SEM and TEM analysis showed that direct interaction of AgCl-NPs with bacterial cells caused rupture of cell wall and cytoplasmic membranes, as well as leakage of nucleic acid materials, which ultimately resulted in the death of R. solanacearum. Overall, these findings will help in developing a promising nanopesticide against phytopathogen plant disease management.

show abstract

“…Essa caracterização inicial da solução de produção como incolor se dá pelo princípio da qual as partículas de pratas são produzidas, pois nesse processo de redução para prata, em que ao ocorrer a transformação do nitrato de prata para as nanopartículas de prata ocorre uma mudança de coloração, em geral para um marrom escuro, que será detectada e quantificada de acordo com a quantidade produzida na síntese (Ravindra et al, 2010;Al-Shabib et al, 2020). Research, Society and Development, v. 10, n. 6, e11710615533.…”

Section: Espectroscopia Síntese E Caracterização Das Nanopartículasunclassified

Preparação, caracterização e aplicação antimicrobiana de nanopartículas de prata estabilizadas em extrato de girassol (Heliantus Annus)

Lemos

Viana

et al. 2021

RSD

View full text Add to dashboard Cite

Este estudo objetivou analisar o efeito do extrato de Helianthus Annuus (AgNP@Ha) na síntese de uma nanopartícula de prata e avaliar sua ação antimicrobiana contra bactérias gram-positivo, gram-negativo e fungos. A produção da nanopartícula foi realizada em agitação sobre uma chapa magnética aquecida até a configuração total da nano, ao final foi utilizado o extrato para a estabilização da mesma, o seu tamanho comprovado através do UV-VIS. A análise microbiológica foi feita em placa com medição do tamanho do halo inibitório formado. Obteve-se uma AgNP@Ha com diâmetros de partículas coloidais com variação de 422 - 414 nm (λmax 422 nm) com efeito de formação de halo sobre as linhagens de S. aureus (16 mm), E. coli (12 mm) e P. aeruginosa (10 mm), enquanto as AgNPs que não foram estabilizadas com o girassol não apresentaram o halo inibitório. Através desta pesquisa foi possível comprovar a melhoria da eficiência de ação e de produção ao se utilizar o extrato de girassol para a estabilização. A síntese de nanopartículas de prata utilizando o extrato das sementes de Helianhtus annuus promoveu uma abordagem mais amigável ao meio ambiente, simples, eficiente e com baixo custo para a obtenção de nanopartículas se tornando um excelente produto com potencial antimicrobiano para ser comercializado além de servir como subsídio para pesquisas posteriores.

show abstract

Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability

Abstract: Novel, safe, and effective antilisterial agents are required in order to prevent Listeria monocytogenes infections and maintain food safety.

Cited by 15 publications

References 54 publications

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity

Biosynthesis of Silver Chloride Nanoparticles by Rhizospheric Bacteria and Their Antibacterial Activity against Phytopathogenic Bacterium Ralstonia solanacearum

Preparação, caracterização e aplicação antimicrobiana de nanopartículas de prata estabilizadas em extrato de girassol (Heliantus Annus)

Contact Info

Product

Resources

About