Interleukin-8 induces cervical ripening in rabbits

Maradny, Emad El; Kanayama, Naohiro; Halim, Abdul; Maehara, Kayoko; Sumimoto, Kazuhiro; Terao, Toshihiko

doi:10.1016/s0002-9378(94)70081-8

Cited by 78 publications

(31 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The decrease in iNOS expression or NO production may be required to allow an increase in MMP-8 and MMP-9 in the later stages of gestation. The increase in COX-2 expression or PG production may be required for parturition and to allow an increase in IL-8, a chemotactic agent that attracts neutrophils that are known to express MMP-8 and MMP-9 (Chwalisz et al 1994;El Maradny et al 1994;Winkler et al 1999;Elliott et al 2001;Watari et al 2003). Figure 9 The expression of iNOS and COX-2 mRNA in rat cervical tissues after prolongation of pregnancy with progesterone.…”

Section: Discussionmentioning

confidence: 99%

Effects of Progesterone on iNOS, COX-2, and Collagen Expression in the Cervix

Marx

Wentz

MacKay

et al. 2006

J Histochem Cytochem.

View full text Add to dashboard Cite

S U M M A R Y This study examines the relationship between inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in the control of cervical ripening and parturition under normal (normal term pregnancy) and abnormal (preterm labor and prolongation of pregnancy) conditions by (a) measuring changes in the collagen both visually and quantitatively, (b) localizing and characterizing iNOS and COX-2 under normal conditions, and (c) characterizing the changes in iNOS and COX-2 under abnormal conditions. Cervices are obtained from estrus and timed pregnant Sprague-Dawley rats (n54-10 per group). Preterm labor is induced with Onapristone (3 mg/rat; progesterone antagonist) and the prolongation of pregnancy with progesterone (2.5 mg, twice daily). Collagen changes are measured and visualized with the picrosirius polarization method. RT-PCR is used to characterize the mRNA expression (p,0.05), and immunohistochemistry is used to localize the protein expression for iNOS and COX-2. The organization and birefringence of the collagen during pregnancy decreased and is supported by changes in the luminosity (p,0.001). The iNOS and COX-2 enzymes were localized in cervical smooth muscle, vascular smooth muscle, and epithelium. Under normal conditions, iNOS mRNA levels decreased as COX-2 mRNA levels increased demonstrating an inverse correlation (Spearman r 5 20.497; p50.00295). Onapristone stimulated preterm labor, increasing the iNOS and COX-2 mRNA (p,0.05). The increase demonstrated a positive correlation (Spearman r 5 0.456; p50.03). Progesterone prolonged pregnancy, decreasing the iNOS and COX-2 mRNA (p50.036). In conclusion, there may be an interaction between the nitric oxide and prostaglandin pathways in cervical ripening and parturition. (J Histochem Cytochem 54:623 -639, 2006)

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Progesterone on iNOS, COX-2, and Collagen Expression in the Cervix

Marx

Wentz

MacKay

et al. 2006

J Histochem Cytochem.

View full text Add to dashboard Cite

show abstract

“…It is well known that IL-8 is a potent chemotactic 267 activator of neutrophils and induces the migration of neutrophils from the vessels to surrounding connective tissue (Peveri et al, 1988). Previous studies have shown that the application of inflammatory cytokines IL-8, IL-1β and TNF-α induce cervical ripening (Chwalisz et al, 1994;Maradny et al, 1994Maradny et al, , 1996, the effects of IL-8 having been shown to be more specific for the uterine cervix (Chwalisz et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…IL-8 has been detected in increasing amounts from the amniotic fluid in term pregnancies, while its production has also been demonstrated in vitro in the human cervix (Romero et al, 1991;Barclay et al, 1993;Axemo et al, 1996). IL-8 is one of the factors which can ripen the cervix in a similar manner to the physiological process at term (Maradny et al, 1994). There is also evidence that IL-8 plays an important role in promoting cervical modification in late pregnancy (Barclay et al, 1993).…”

mentioning

confidence: 99%

Binding of interleukin-8 to heparan sulphate enhances cervical maturation in rabbits

Belayet

Kanayama

Khatun

et al. 1999

Molecular Human Reproduction

View full text Add to dashboard Cite

Cervical ripening is a cytokine-trigged process with substantial remodelling of the cervical extracellular matrix. Interleukin-8 (IL-8) is an important cytokine in cervical maturation. Glycosaminoglycans are also included in this process, but their role in not clearly understood. The effects of heparan sulphate (HS), hyaluronic acid (HA), IL-8, HS ⍣ IL-8 and HA ⍣ IL-8 on biochemical properties of the cervix were examined in non-pregnant rabbits. The changes in vascular pattern with collagen structure of the cervices and immunohistochemical studies, together with the relative collagen concentrations, were determined. A reduction in relative collagen concentration was significant after HS ⍣ IL-8 , IL-8 and HA ⍣ IL-8 treatment (all P < 0.0001). Gel electrophoresis analysis showed that IL-8 bound preferentially to HS than to HA. Neutrophils were significantly increased in number (P < 0.0001) and located predominantly beneath the glandular epithelium and around the blood vessels after HS ⍣ IL-8 treatment. HS ⍣ IL-8 treatment caused cervices to increase their water content and become oedematous. The collagen fibres were considerably dissociated, the interfibrillar spaces markedly dilated, and the blood vessels notably increased and dilated. We conclude that binding to HS enhances the activity of IL-8 in inducing cervical maturation. Key words: cervical maturation/heparan sulphate/hyaluronic acid/interleukin-8/neutrophil activation IntroductionThe non-pregnant cervix is a fibrous structure which undergoes modifications during pregnancy to allow sufficient softening and growth for the passage of the fetus at birth (Harkness and Harkness, 1959;Friedman, 1980;Calder, 1981;Fitzpatrick, 1981). It is well established that the transformation of the uterine cervix to a soft and compliant structure during pregnancy is crucial for a normal delivery. This ripening process can be explained mainly in terms of connective tissue biology (Huszar and Walsh, 1991). Investigations of the structural alterations of cervical connective tissue during pregnancy and labour have focused predominantly on quantitative changes of the collagen content and the role of collagen-degraded enzymes (Ito et al., 1979;Danforth, 1980;Uldbjerg et al., 1983). However, there are strong indications that other macromolecular components of the cervix, such as proteoglycans (PG), may also be involved (von Maillot et al., 1979;Kitamura et al., 1980). PG are core proteins with covalently attached glycosaminoglycan (GAG) chains (Ruoslahti, 1988). In addition to heparin/ heparan sulphate (HS), chondroitin sulphate/dermatan sulphate, keratan sulphate and hyaluronic acid (HA) have also been isolated from the human cervix (von Maillot et al., 1979;Cabrol et al., 1980;Kitamura et al., 1980;Uldbjerg et al., 1983). A marked increase in the GAG content of the cervix during late pregnancy has been demonstrated in rats, rabbits, sheep and humans (Danforth et al., 1974;Golichowski et al., © European Society of Human Reproduction and Embryology 261 1980; Fosang et al., 1984;Mara...

show abstract

“…The mechanism of action of DHA-S in the production of cervical collagenase may involve the expression of IL-8 R induced by DHA-S. IL-8 is a potent chemotactic and activating cytokine for neutrophils. Recently, we found that the local application of exogenous IL-8 induced marked cervical maturation (20). Neutrophil infiltration is important in the process of cervical maturation.…”

Section: Il-8 Il-8 Rmentioning

confidence: 98%

“…Effects of IL-8 are mediated by binding to two types of specific high affinity receptors, I and II. Exogenous application of IL-8 to the cervix in rabbits is found to induce ripening (20). The uterine cervix in humans and rabbits produces large amounts of IL-8, especially late in pregnancy (21,22).…”

Section: Introductionmentioning

confidence: 99%

Effect of dehydroepiandrosterone sulfate on interleukin-8 receptor during cervical ripening

Kanayama

Maradny

Goto

et al. 1998

European Journal of Endocrinology

View full text Add to dashboard Cite

We investigated the effects of dehydroepiandrosterone sulfate (DHA-S) on the production of interleukin-8 (IL-8) and expression of the interleukin-8 receptor (IL-8 R) in human cervical tissue. DHA-S increased the levels of IL-8 in cultured human cervical fibroblasts and in the supernatant in a timeand dose-dependent manner. DHA-S induced IL-8 and IL-8 R expression in human cervical fibroblasts and human pregnant cervical tissue at term in a dose-dependent manner. In addition, it induced the expression of IL-8 R in an explant culture of human cervical tissue and cultured human cervical fibroblasts in a time-and dose-dependent manner. However, dehydroepiandrosterone (DHA) only slightly induced the production of IL-8 and the expression of IL-8 R in the same cells and tissue. These results suggest that DHA-S up-regulates the autocrine system of IL-8 through the expression of IL-8 R.

show abstract

Interleukin-8 induces cervical ripening in rabbits

Abstract: Interleukin-8 can induce cervical ripening in nonpregnant and pregnant rabbits.

Cited by 78 publications

References 17 publications

Effects of Progesterone on iNOS, COX-2, and Collagen Expression in the Cervix

Effects of Progesterone on iNOS, COX-2, and Collagen Expression in the Cervix

Binding of interleukin-8 to heparan sulphate enhances cervical maturation in rabbits

Effect of dehydroepiandrosterone sulfate on interleukin-8 receptor during cervical ripening

Contact Info

Product

Resources

About