Inhibitory Effect of Rumex Crispus L. Fraction on Adipocyte Differentiation in 3T3-L1 Cells

Abstract

The anti-obesity effect of ethanol extract and their fractions from Rumex Crispus L. on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil red O assay, western blot and real-time PCR analysis. Ethyl acetate fraction of Rumex crispus L. significantly inhibited adipocyte differentiation when treated during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil red O staining. In inducing differentiation of 3T3-L1 preadipocytes in the presence of an adipogenic cocktail, isobutylmethylxanthine (IBMX), dexamethasone-and insulin-along with ethyl acetate fraction residue processing treatment significantly decreased protein expression of obesity-related proteins, such as peroxisome-proliferatorsactivated-receptor-γ (PPARγ) and CCAAT enhancer-binding-proteins α (C/EBPα). These results indicate that ethyl acetate fraction of Rumex crispus L. is the most effective candidate for preventing obesity. However further studies will be needed to identify the active compounds that confer the anti-obesity activity of ethyl acetate fraction from Rumex crispus L.

Novel Peptides in Fermented Milk with Specific Lactobacillus Strains Potential Antiobesity Effect: In Vitro and in Silico Analysis

“…In general, most studies have reported that specific probiotic bacteria or even their cell components have shown adipogenesis inhibitory activity. ,, In fact, to the best of our knowledge, only few studies have evaluated adipogenesis inhibition of FM or its hydrolysates in in vitro or in vivo studies. , In this regard, Ho et al evaluated different kefir fractions and observed a 40% inhibition of lipid accumulation in 3T3-L1 cells. Similarly, Hyun et al reported that two different skimmed milks fermented by two different LAB strains inhibited the differentiation of 3T3-L1 cells by significantly inhibiting the accumulation of lipid droplets in a concentration-dependent manner.…”

Isolation of Lactobacillus HBUAS56719 from Black Raspberry Leaves and its in-vitro antidiabetic activity

“…These Lactobacillus spp. strains could reduce the absorption of intestinal carbohydrates (Park et al, 2018).…”

Rumex crispus and Cordyceps militaris Mixture Ameliorates Production of Pro-Inflammatory Cytokines Induced by Lipopolysaccharide in C57BL/6 Mice Splenocytes

“…Rc is commonly consumed as a dried or processed herb or used as a medicinal product (11), and is known to inhibit arachidonic acid induced inflammation in mice (12). Rc also protects against liver injury (12) and exhibits antioxidant (13), anti-cancer (14) and anti-obesity (15) effects. Similarly, Cordyceps militaris (Cm) has been used since ancient times as an herbal ingredient (16) and has been reported to have anti-inflammatory and anti-cancer effects (11,17).…”