Novel Peptides in Fermented Milk with Specific Lactobacillus Strains Potential Antiobesity Effect: In Vitro and in Silico Analysis

Abstract

The objective was to investigate the potential antiobesity effect of fermented milks (FMs) with 11 strains of specific Lactobacillus strains isolated from artisanal Mexican cheeses. First, pancreatic lipase inhibitory activity (PLIA) was determined in all FMs. Then, the FM with the most PLIA was selected for peptide identification by high-performance liquid chromatography (HPLC)/tandem mass spectrometry (MS–MS) and for adipogenesis studies. FMs with Limosilactobacillus fermentum J20 (J20) displayed the highest (p < 0.05) PLIA (81.70 ± 2.24%) and the lowest IC50 (0.05 0.05 mg/mL) of all. Also, FM J20 inhibited lipid accumulation in a 3T3-L1 cell line. Eight novel identified peptides released by in silico digestion from the fraction with the most PLIA (p < 0.05) presented sequences that strongly bind enzyme active sites and may be responsible for lipid accumulation inhibition during adipogenesis. Thus, FM J20 may be used for the development of a dairy product with potential antiobesity effects.

Fermented traditional wine from palm trees: microbial, nutritional attributes and health impacts

“…Lactobacillus strains such as Limosilactobacillus fermentum (Naghmouchi et al, 2020), Lactiplantibacillus plantarum L3 (Wang et al, 2023), Lactiplantiblacillus plantarum A3 and Lacticaseibacillus rhamnosus ATCC7469 (Li et al, 2023), Lacticaseibacillus paracasei (Bengoa et al, 2023), Lactiplantibacillus paraplantarum (Coimbra- Gomes et al, 2023), Lactobacillus rhamnosus B2-1 (Guo et al, 2023), and Limosilactobacillus fermentum (Paulino do Nascimento et al, 2022) are involved in the manufacture of several fermented foods for health-promoting effects (Nath et al, 2020;Widyastuti et al, 2021;Manzanarez-Quin et al, 2023) and acting as probiotics (Lynch et al, 2018;Lee et al, 2022;Coimbra-Gomes et al, 2023). Some strains of lactobacilli were reported to have probiotic activities that are useful in the prevention and treatment of gastro-intestinal disorders, such as rotavirus diarrhoea, antibiotic-associated diarrhoea, and travellers' diarrhoea, they can act as potential therapeutic agents to treat inflammatory bowel disease and irritable bowel syndrome (Kim et al, 2017;Levit et al, 2019;Krammer et al, 2021).…”

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“…The results presented by the authors demonstrated that multistrain culture improved fermentation capacity; however, when milk was fermented with only Lactiplantibacillus plantarum (Lp39), the product showed higher antioxidant and anti-inflammatory activities, suggesting that the product could exert positive health effects. Moreover, Manzanarez-Quin et al 5 reported the anti-obesogenic effect of milk fermented with Lactobacillus strains isolated from artisanal Mexican cheeses, and the authors associated the bioactivity with eight novel identified peptides.…”