Isolation of Lactobacillus HBUAS56719 from Black Raspberry Leaves and its in-vitro antidiabetic activity

Abstract

Probiotics are living bacteria that, when given in sufficient quantities, help the host's health. They are currently recognized as appropriate substitutes for antibiotics in medical practice of animal diseases and the improvement in animal productivity. It has several different pharmacological properties, including as anti-diabetic, anti-inflammatory, and antioxidant actions. The aim of this article was to characterize of L. plantarum HBUAS56719 isolated from fermented black raspberry and estimate its antidiabetic activity. In the current investigation, L. plantarum HBUAS56719 was isolated from black raspberry leaves aqueous extract (BRLAE) and characterized using bile salt tolerance assay, cell-surface adhesion to solvents affinity, hemolytic test, 16S rDNA sequencing, and PCR-amplified product electrophoresis on agarose gel. On the other hand, survival rates, adhesion assay, of L. plantarum HBUAS56719 in bile salt, hemolytic test L. plantarum HBUAS56719 were estimated. However, α-glucosidase, pancreatic lipase, lipoprotein lipase inhibitory activity of L. plantarum HBUAS56719 were evaluated to access its anti-obesity activity. The results of our study showed that the agarose gel electrophoresis of L. plantarum HBUAS56719 using PCR results, a fragment (1500 bp) of 16S rRNA. Also, survival rates of Lactiplantibacillus sp. in bile salt (0.1 -0.8%) showed that 0.8% oxgall produces the highest ratio of bile acid tolerance 95%. Also, adhesion assay of L. plantarum sp. to solvents (n-hexadecane chloroform and ethyl acetate) revealed that low acidic characteristics (less than 40% affinity to n-hexadecane and ethyl acetate) and highest basic characteristics (more than 60 % affinity to chloroform). In addition, L. plantarum HBUAS56719 was γ-hemolytic and did not exhibit any hemolysis on the agar blood plates. Our results showed that α-glucosidase, pancreatic lipase and lipoprotein lipase inhibitory activities of L plantarum HBUAS56719 was in the order of 91, 29 and 48%, respectively. The findings imply that L. plantarum HBUAS56719 could be explored to produce α-glucosidase, pancreatic lipase and lipoprotein lipase inhibitory peptides from fermented black raspberry. Fermented black raspberry produced by L. plantarum HBUAS56719 could be a novel source of antidiabetic peptides.