Inhibitory Effect of Ethanolic Extract of Curanga Fel-Terrae (Pugun Tano) Leaves on Acetylcholine Muscarinic-3 Receptors Induced on Isolated Guinea Pig Tracheal

Abstract

Objective: The study was aimed to investigate the inhibitory effect of ethanolic extract of Curanga fel-terrae leaves (EECFL) against acetylcholine (ACh)-induced contraction of the ACh-muscarinic-3 receptor. Materials and Methods:The study of the inhibitory effect of the ethanolic extract on the contraction by ACh concentration series (10 −8 -10 −3 M) was conducted in vitro using isolated guinea pig tracheal organ in the Krebs solution.Results: Early incubation of tracheal organ with EECFL (0.5, 1, 2 and 4 mg/ml) before contracted by the series of ACh concentration produces the decrease of ACh contraction. The concentration series of ACh sigmoid curve rightward shift without decreasing the maximal contraction. The results of double-reciprocal plot of ACh shows the mean value of the 1/y-intercept of each extract was not different with the control. Conclusion:The EECFL showed competitive antagonist effect on ACh-muscarinic-3 receptors.

Ethanolic Extract of Herb Pugun Tanoh (Picria fel-terrae Lour.) Modulates TCD4+ and TCD8+ Cell Profile of Doxorubicin-Induced Immuno-Suppressed Rats

“…+ due to Dox administration. Preliminary phytochemical screening of ethanolic extract of Picria fel-terrae leaves indicated that the presences of flavonoids, saponins, triterpenes, and steroids [25]. Some flavonoids showed anticancer and immunomodulatory activity.…”

“…Reportedly showed that this plant contained glycoside, flavonoid, saponin, terpenoid, curangin, and bitter compound [2], [3], [4], [5], [6], [7], [8]. The pharmacological activity of this plant also has been studied such as anthelmintic, antidiabetic, anti-breast cancer, diuretic effect, cardioprotective effect, and antimuscarinic receptor [2], [9], [14].…”

Effectiveness of Pugun Tanoh Leaves Ethanol Extract (Curanga fel-terrae (Lour.) Merr) as an Anti-inflammation in Patients after Tooth Extraction at Dental and Mouth Hospital of Dentistry Faculty, Universitas Sumatera Utara

“…In Indonesia, these plants are spread in Sumatra, Java, Kalimantan, and Maluku. C. fel-terrae often used by the Karo tribe of Tiga Lingga Village, Kabupaten Dairi, Provinsi Sumatera Utara to treat diarrhea, asthma, cough, scabies, inflammation, bruising, and abdominal pain [5]. C. fel-terrae has many benefits for treating various diseases not only empirically proven but also scientifically.…”

PHYTOCHEMICAL CONSTITUENT OF Picria fel-terrae Lour. ETHANOL EXTRACT AND ITS TERATOGENIC EFFECT

“…Some researches have been carried out to this plant and reported has many activities such as a diuretic, acetylcholinesterase inhibitor, hepatoprotective, anticancer, antidiabetic, antiasthma as well as anthelmintic. [1][2][3][4][5][6][7][8][9] The various benefits derived from P. fel-terrae as a medicinal plant are potential to be developed into a safe and easy-to-use herbal medicine preparation including for pregnant women. To obtain herbal medicine which safe for human use, a series of toxicity tests are required.…”