Inhibition of Struvite Crystal Growth in the Presence of Herbal Extract<i>Orthosiphon Aristatus</i>BL.MIQ

Muryanto, S.; Sutanti, Sri; Kasmiyatun, Mega

doi:10.1051/matecconf/20165801013

Cited by 4 publications

(2 citation statements)

References 13 publications

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“…The phosphate crystals, known as struvite (MgNH4PO4.6H2O -Ammonium Magnesium Phosphate Hexahydrate) is a bio-mineral (7,8) formed due to urinary tract infections (9). Struvites account for 10-20% of all renal calculi (10) and are found more frequently in women and persons older than 50 years (11).…”

Section: Introductionmentioning

confidence: 99%

In Vitro evaluation of anti-urolithiatic activity of Piper betle L.

Alwe,

Gavas

2024

IJAM

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Urolithiasis or kidney stone is the most frequently occurring urinary disorders seen all over the world. They are saturated crystals formed due to the minerals present in the urine. It majorly comprises calcium oxalate, uric acid and phosphate crystals. The phosphate crystals, known as struvite, are formed due to the urinary tract infections. Although there are various synthetic medications, the use of traditional medicines / plants has been a promising method to treat kidney stones. To investigate the antiurolithiatic potential of traditional plant - Piper betle, the aqueous extract of Piper betle leaves was used. This extract was tested on two different crystals, first onto the raphides present in Colocasia esculenta leaf which is analogous to the calcium oxalate crystals. Second on the struvite crystals prepared from the crystallization method. The study showed there was a considerable decrease in the amount of raphides present in Colocasia esculenta and the same effect was seen on struvite crystals, which reveals that the leaves of Piper betle have an effect in dissolving and/or minimising the size of both crystals, thus substantiating the traditional claim. It is concluded that the leaves of Piper betle have beneficial inhibitory effect on in vitro crystallization of calcium oxalate and struvite crystals.

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Section: Introductionmentioning

confidence: 99%

In Vitro evaluation of anti-urolithiatic activity of Piper betle L.

Alwe,

Gavas

2024

IJAM

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“…In an alkaline state, supersaturated urine leads to nucleation, crystal growth and aggregation [8] thus, reduces the solubility of ammonium magnesium phosphate and leads to formation and deposition of struvite crystals [9]. Urea-splitting urolithic microorganisms, Proteus, Pseudomonas, Mycoplasma, Klebsiella, Staphylococcus, and yeast can hydrolyze urea to ammonia and raise the alkaline nature (pH) of urine [10,11]. Infection stones, urease, triple-phosphate stones and magnesium ammonium phosphate stone are the common names of struvite stone.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract

Jeyaleela¹,

Vimala²,

Senthil

et al. 2019

Asian J. Chem.

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Present work aimed to isolate bioactive principle and its inhibitory potential against struvite stone by single diffusion method as well as docking studies to determine the mode of interaction between the complex structure of urease and rutin. Flavonoid compound is isolated from Melia dubia leaf extract with the help of TLC and column chromatography, and then characterized by 1H & 13C NMR, and LC-MS. Inhibitory potential of isolated flavonoid against kidney stone (struvite) is confirmed by molecular docking approach. Rutin acts as a urease inhibitor and the residues of urease namely 463Asp and 466 Ala were found to bind with rutin through hydrogen bonding and van der Waals interactions. In vitro study, the results revealed that with an increase in concentration of flavonoid rutin (1-5 mg/mL) the weight of the harvested crystals were gradually reduced from 2.8 cm to 0.5 cm and weight 0.662 g to 0.322 g at 5 mg/mL concentration. The XRD result of C+5 shows well inhibition on struvite crystal in all the planes and in SEM analysis, rutin inhibited crystal, surface of the crystal was damaged, whereas control shows pure and no defects on it.

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Current insights into the mechanisms and management of infection stones

et al. 2018

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Infection stones are complex aggregates of crystals amalgamated in an organic matrix that are strictly associated with urinary tract infections. The management of patients who form infection stones is challenging owing to the complexity of the calculi and high recurrence rates. The formation of infection stones is a multifactorial process that can be driven by urine chemistry , the urine microenvironment, the presence of modulator substances in urine, associations with bacteria, and the development of biofilms. Despite decades of investigation, the mechanisms of infection stone formation are still poorly understood. A mechanistic understanding of the formation and growth of infection stones-including the role of organics in the stone matrix, microorganisms, and biofilms in stone formation and their effect on stone characteristics-and the medical implications of these insights might be crucial for the development of improved treatments. Tools and approaches used in various disciplines (for example, engineering, chemistry , mineralogy , and microbiology) can be applied to further understand the microorganism-mineral interactions that lead to infection stone formation. Thus, the use of integrated multidisciplinary approaches is imperative to improve the diagnosis, prevention, and treatment of infection stones.

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Inhibition of Struvite Crystal Growth in the Presence of Herbal ExtractOrthosiphon AristatusBL.MIQ

Cited by 4 publications

References 13 publications

In Vitro evaluation of anti-urolithiatic activity of Piper betle L.

In Vitro evaluation of anti-urolithiatic activity of Piper betle L.

Isolation, Characterization, Molecular Docking and in vitro Studies of Inhibitory Effect on the Growth of Struvite Crystal Derived from Melia dubia Leaf Extract

Current insights into the mechanisms and management of infection stones

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