Medicinal plants engrossed great attention for the synthesis of new chemical entities and its biomolecules has fabulous application in many fields such as corrosion, nanotechnology, pharmaceutical companies, and cosmetic industries. The main purpose of the present work is biosynthesis of zinc oxide nanoparticles via simple homogeneous precipitation method using the isolated flavonoid quercetin, which was derived from the medicinal plant Combretum ovalifolium. Antioxidant activity and photocatalytic degradation of methylene blue dye were studied on the synthesized ZnONPs. Zinc acetate dihydrate (Zn (CH 3 COO) 2 .2H 2 O) was utilized as a precursor and quercetin as a reducing agent. Formed Quercetin mediated zinc oxide nanoparticles (Q-ZnONPs) were characterized by UV-Visible, FT-IR, XRD, EDX, and SEM. Fiber shaped Q-ZnONPs were obtained with single crystalline nature, its average diameter was found to be 31.24nm. The results shows that, 168.045µg/ml of Q-ZnONPs scavenged 50% of DPPH on antioxidant activity. The degradation behaviour of the nanomaterial was analysed. The results showed that on the addition of biosynthesized Q-ZnONPs, the degradation of methylene blue increased up to 76.01% at 240 minutes.
Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubai Methods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound.
Conclusion:The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.
Present work aimed to isolate bioactive principle and its inhibitory potential against struvite stone by single diffusion method as well as docking studies to determine the mode of interaction between the complex structure of urease and rutin. Flavonoid compound is isolated from Melia dubia leaf extract with the help of TLC and column chromatography, and then characterized by 1H & 13C NMR, and LC-MS. Inhibitory potential of isolated flavonoid against kidney stone (struvite) is confirmed by molecular docking approach. Rutin acts as a urease inhibitor and the residues of urease namely 463Asp and 466 Ala were found to bind with rutin through hydrogen bonding and van der Waals interactions. In vitro study, the results revealed that with an increase in concentration of flavonoid rutin (1-5 mg/mL) the weight of the harvested crystals were gradually reduced from 2.8 cm to 0.5 cm and weight 0.662 g to 0.322 g at 5 mg/mL concentration. The XRD result of C+5 shows well inhibition on struvite crystal in all the planes and in SEM analysis, rutin inhibited crystal, surface of the crystal was damaged, whereas control shows pure and no defects on it.
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